Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0031 and Psyr_0032 are separated by 266 nucleotides Psyr_0032 and Psyr_0033 are separated by 125 nucleotides Psyr_0033 and Psyr_0034 overlap by 4 nucleotides
Psyr_0031: Psyr_0031 - Alpha/beta hydrolase fold protein, at 35,445 to 36,269
_0031
Psyr_0032: Psyr_0032 - conserved hypothetical protein, at 36,536 to 37,066
_0032
Psyr_0033: Psyr_0033 - tryptophan synthase, alpha chain, at 37,192 to 38,001
_0033
Psyr_0034: Psyr_0034 - tryptophan synthase, beta chain, at 37,998 to 39,227
_0034
Position (kb) 37
38
39 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 36.327 kb on + strand at 36.327 kb on + strand at 36.328 kb on - strand at 36.328 kb on - strand at 36.328 kb on - strand at 36.340 kb on + strand at 36.340 kb on + strand at 36.341 kb on - strand at 36.341 kb on - strand at 36.342 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.456 kb on + strand at 36.564 kb on + strand at 36.564 kb on + strand at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.645 kb on + strand, within Psyr_0032 at 36.646 kb on - strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.714 kb on - strand, within Psyr_0032 at 36.714 kb on - strand, within Psyr_0032 at 36.902 kb on - strand, within Psyr_0032 at 37.045 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.176 kb on - strand at 37.207 kb on - strand at 37.207 kb on - strand at 37.210 kb on + strand at 37.210 kb on + strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.478 kb on + strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.697 kb on + strand, within Psyr_0033 at 37.991 kb on - strand at 37.991 kb on - strand at 38.002 kb on - strand at 38.234 kb on - strand, within Psyr_0034 at 38.234 kb on - strand, within Psyr_0034 at 38.234 kb on - strand, within Psyr_0034 at 38.350 kb on + strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.648 kb on - strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.920 kb on - strand, within Psyr_0034
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 36,327 + +0.8 36,327 + -0.7 36,328 - -0.6 36,328 - -0.4 36,328 - +0.1 36,340 + +0.7 36,340 + -0.3 36,341 - +1.2 36,341 - -2.1 36,342 + -0.2 36,454 + -0.4 36,454 + +0.5 36,454 + -2.4 36,454 + -0.1 36,454 + -0.9 36,454 + -0.4 36,454 + -0.7 36,454 + +0.2 36,454 + -0.2 36,454 + -0.5 36,454 + +1.3 36,455 - +0.1 36,455 - +0.7 36,455 - +0.8 36,455 - -0.1 36,456 + +1.3 36,564 + -0.8 36,564 + +0.2 36,633 + Psyr_0032 0.18 -1.1 36,633 + Psyr_0032 0.18 +0.5 36,633 + Psyr_0032 0.18 -0.8 36,633 + Psyr_0032 0.18 -0.7 36,634 - Psyr_0032 0.18 -0.3 36,634 - Psyr_0032 0.18 -1.3 36,634 - Psyr_0032 0.18 -0.2 36,634 - Psyr_0032 0.18 -0.3 36,634 - Psyr_0032 0.18 -1.2 36,645 + Psyr_0032 0.21 +0.2 36,646 - Psyr_0032 0.21 +3.5 36,713 + Psyr_0032 0.33 -1.0 36,713 + Psyr_0032 0.33 +1.4 36,713 + Psyr_0032 0.33 -0.2 36,714 - Psyr_0032 0.34 -0.3 36,714 - Psyr_0032 0.34 +0.7 36,902 - Psyr_0032 0.69 +1.7 37,045 + +0.4 37,140 + -0.2 37,140 + -0.5 37,140 + -0.8 37,140 + -1.0 37,140 + +0.2 37,140 + -0.7 37,140 + +0.9 37,140 + +0.4 37,140 + -2.5 37,140 + -0.8 37,140 + -1.1 37,141 - +0.7 37,141 - +0.2 37,141 - -0.8 37,141 - +0.6 37,141 - -0.2 37,141 - +0.2 37,141 - -0.3 37,141 - +0.2 37,141 - -0.3 37,141 - -0.1 37,144 + -0.9 37,144 + -0.5 37,144 + -1.5 37,144 + +0.4 37,144 + +0.2 37,144 + -0.7 37,144 + -0.4 37,145 - +1.8 37,145 - -1.6 37,145 - -1.4 37,145 - -0.1 37,145 - -0.1 37,145 - -0.1 37,145 - +3.3 37,145 - -0.7 37,145 - -1.3 37,176 - +1.1 37,207 - +0.6 37,207 - -0.9 37,210 + -0.5 37,210 + -1.1 37,211 - +0.5 37,211 - -0.6 37,211 - -0.2 37,211 - -0.0 37,211 - -1.2 37,475 + Psyr_0033 0.35 -2.2 37,475 + Psyr_0033 0.35 -0.9 37,475 + Psyr_0033 0.35 +0.9 37,475 + Psyr_0033 0.35 -0.7 37,476 - Psyr_0033 0.35 +2.1 37,476 - Psyr_0033 0.35 -2.8 37,476 - Psyr_0033 0.35 +0.1 37,478 + Psyr_0033 0.35 +0.2 37,695 - Psyr_0033 0.62 -0.2 37,695 - Psyr_0033 0.62 +0.0 37,695 - Psyr_0033 0.62 +0.2 37,695 - Psyr_0033 0.62 -1.0 37,697 + Psyr_0033 0.62 -0.7 37,991 - +2.4 37,991 - -0.2 38,002 - +0.4 38,234 - Psyr_0034 0.19 -1.2 38,234 - Psyr_0034 0.19 -2.3 38,234 - Psyr_0034 0.19 +0.7 38,350 + Psyr_0034 0.29 -0.5 38,351 - Psyr_0034 0.29 +0.2 38,351 - Psyr_0034 0.29 -0.7 38,351 - Psyr_0034 0.29 -0.6 38,648 - Psyr_0034 0.53 -1.8 38,806 + Psyr_0034 0.66 -0.1 38,806 + Psyr_0034 0.66 -1.4 38,806 + Psyr_0034 0.66 -1.2 38,807 - Psyr_0034 0.66 -0.5 38,807 - Psyr_0034 0.66 +2.9 38,807 - Psyr_0034 0.66 -0.4 38,807 - Psyr_0034 0.66 -0.9 38,807 - Psyr_0034 0.66 +0.9 38,807 - Psyr_0034 0.66 +0.4 38,920 - Psyr_0034 0.75 +0.9
Or see this region's nucleotide sequence