Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0004 and Psyr_0005 are separated by 55 nucleotides Psyr_0005 and Psyr_0006 are separated by 27 nucleotides
Psyr_0004: Psyr_0004 - DNA gyrase subunit B, at 3,809 to 6,226
_0004
Psyr_0005: Psyr_0005 - conserved hypothetical protein, at 6,282 to 7,169
_0005
Psyr_0006: Psyr_0006 - putative phenylacetaldoxime dehydratase, at 7,197 to 8,231
_0006
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6.233 kb on + strand at 6.233 kb on + strand at 6.233 kb on + strand at 6.234 kb on - strand at 6.234 kb on - strand at 6.284 kb on - strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.345 kb on + strand at 6.346 kb on - strand at 6.346 kb on - strand at 6.346 kb on - strand at 6.346 kb on - strand at 6.346 kb on - strand at 6.346 kb on - strand at 6.346 kb on - strand at 6.346 kb on - strand at 6.346 kb on - strand at 6.346 kb on - strand at 6.346 kb on - strand at 6.415 kb on + strand, within Psyr_0005 at 6.415 kb on + strand, within Psyr_0005 at 6.415 kb on + strand, within Psyr_0005 at 6.415 kb on + strand, within Psyr_0005 at 6.416 kb on - strand, within Psyr_0005 at 6.416 kb on - strand, within Psyr_0005 at 6.416 kb on - strand, within Psyr_0005 at 6.416 kb on - strand, within Psyr_0005 at 6.419 kb on + strand, within Psyr_0005 at 6.419 kb on + strand, within Psyr_0005 at 6.420 kb on - strand, within Psyr_0005 at 6.420 kb on - strand, within Psyr_0005 at 6.420 kb on - strand, within Psyr_0005 at 6.420 kb on - strand, within Psyr_0005 at 6.420 kb on - strand, within Psyr_0005 at 6.420 kb on - strand, within Psyr_0005 at 6.476 kb on + strand, within Psyr_0005 at 6.477 kb on - strand, within Psyr_0005 at 6.520 kb on + strand, within Psyr_0005 at 6.520 kb on + strand, within Psyr_0005 at 6.521 kb on - strand, within Psyr_0005 at 6.521 kb on - strand, within Psyr_0005 at 6.521 kb on - strand, within Psyr_0005 at 6.521 kb on - strand, within Psyr_0005 at 6.529 kb on + strand, within Psyr_0005 at 6.530 kb on - strand, within Psyr_0005 at 6.530 kb on - strand, within Psyr_0005 at 6.583 kb on + strand, within Psyr_0005 at 6.584 kb on - strand, within Psyr_0005 at 6.658 kb on + strand, within Psyr_0005 at 6.658 kb on + strand, within Psyr_0005 at 6.658 kb on + strand, within Psyr_0005 at 6.658 kb on + strand, within Psyr_0005 at 6.658 kb on + strand, within Psyr_0005 at 6.659 kb on - strand, within Psyr_0005 at 6.659 kb on - strand, within Psyr_0005 at 6.659 kb on - strand, within Psyr_0005 at 6.659 kb on - strand, within Psyr_0005 at 6.659 kb on - strand, within Psyr_0005 at 6.659 kb on - strand, within Psyr_0005 at 6.659 kb on - strand, within Psyr_0005 at 6.659 kb on - strand, within Psyr_0005 at 6.659 kb on - strand, within Psyr_0005 at 6.721 kb on + strand, within Psyr_0005 at 6.721 kb on + strand, within Psyr_0005 at 6.740 kb on - strand, within Psyr_0005 at 6.740 kb on - strand, within Psyr_0005 at 6.740 kb on - strand, within Psyr_0005 at 6.770 kb on + strand, within Psyr_0005 at 6.770 kb on + strand, within Psyr_0005 at 6.770 kb on + strand, within Psyr_0005 at 6.770 kb on + strand, within Psyr_0005 at 6.770 kb on + strand, within Psyr_0005 at 6.770 kb on + strand, within Psyr_0005 at 6.770 kb on + strand, within Psyr_0005 at 6.771 kb on - strand, within Psyr_0005 at 6.771 kb on - strand, within Psyr_0005 at 6.771 kb on - strand, within Psyr_0005 at 6.771 kb on - strand, within Psyr_0005 at 6.783 kb on + strand, within Psyr_0005 at 6.900 kb on + strand, within Psyr_0005 at 6.901 kb on - strand, within Psyr_0005 at 6.901 kb on - strand, within Psyr_0005 at 6.937 kb on + strand, within Psyr_0005 at 6.937 kb on + strand, within Psyr_0005 at 6.937 kb on + strand, within Psyr_0005 at 6.937 kb on + strand, within Psyr_0005 at 6.938 kb on - strand, within Psyr_0005 at 6.938 kb on - strand, within Psyr_0005 at 6.938 kb on - strand, within Psyr_0005 at 6.938 kb on - strand, within Psyr_0005 at 7.001 kb on - strand, within Psyr_0005 at 7.030 kb on + strand, within Psyr_0005 at 7.031 kb on - strand, within Psyr_0005 at 7.070 kb on - strand, within Psyr_0005 at 7.125 kb on - strand at 7.169 kb on + strand at 7.169 kb on + strand at 7.170 kb on - strand at 7.170 kb on - strand at 7.170 kb on - strand at 7.170 kb on - strand at 7.188 kb on + strand at 7.189 kb on - strand at 7.246 kb on + strand at 7.247 kb on - strand at 7.247 kb on - strand at 7.283 kb on - strand at 7.317 kb on + strand, within Psyr_0006 at 7.317 kb on + strand, within Psyr_0006 at 7.317 kb on + strand, within Psyr_0006 at 7.317 kb on + strand at 7.317 kb on + strand, within Psyr_0006 at 7.317 kb on + strand, within Psyr_0006 at 7.317 kb on + strand, within Psyr_0006 at 7.318 kb on - strand, within Psyr_0006 at 7.318 kb on - strand, within Psyr_0006 at 7.318 kb on - strand, within Psyr_0006 at 7.318 kb on - strand, within Psyr_0006 at 7.318 kb on - strand, within Psyr_0006 at 7.321 kb on + strand, within Psyr_0006 at 7.322 kb on - strand, within Psyr_0006 at 7.322 kb on - strand, within Psyr_0006 at 7.322 kb on - strand, within Psyr_0006 at 7.535 kb on + strand, within Psyr_0006 at 7.536 kb on + strand, within Psyr_0006 at 7.536 kb on + strand, within Psyr_0006 at 7.536 kb on + strand, within Psyr_0006 at 7.536 kb on + strand, within Psyr_0006 at 7.536 kb on + strand, within Psyr_0006 at 7.536 kb on + strand, within Psyr_0006 at 7.536 kb on + strand, within Psyr_0006 at 7.536 kb on + strand, within Psyr_0006 at 7.536 kb on + strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.537 kb on - strand, within Psyr_0006 at 7.550 kb on - strand, within Psyr_0006 at 7.550 kb on - strand, within Psyr_0006 at 7.628 kb on + strand, within Psyr_0006 at 7.628 kb on + strand, within Psyr_0006 at 7.628 kb on + strand, within Psyr_0006 at 7.628 kb on + strand, within Psyr_0006 at 7.628 kb on + strand, within Psyr_0006 at 7.628 kb on + strand, within Psyr_0006 at 7.628 kb on + strand, within Psyr_0006 at 7.628 kb on + strand, within Psyr_0006 at 7.629 kb on - strand, within Psyr_0006 at 7.629 kb on - strand, within Psyr_0006 at 7.629 kb on - strand, within Psyr_0006 at 7.629 kb on - strand, within Psyr_0006 at 7.629 kb on - strand, within Psyr_0006 at 7.629 kb on - strand, within Psyr_0006 at 7.629 kb on - strand, within Psyr_0006 at 7.629 kb on - strand, within Psyr_0006 at 7.629 kb on - strand, within Psyr_0006 at 7.676 kb on + strand, within Psyr_0006 at 7.676 kb on + strand, within Psyr_0006 at 7.676 kb on + strand, within Psyr_0006 at 7.677 kb on - strand, within Psyr_0006 at 7.710 kb on - strand, within Psyr_0006 at 7.749 kb on - strand, within Psyr_0006 at 7.849 kb on - strand, within Psyr_0006 at 7.849 kb on - strand, within Psyr_0006 at 7.963 kb on - strand, within Psyr_0006 at 7.970 kb on + strand, within Psyr_0006 at 7.970 kb on + strand, within Psyr_0006 at 8.098 kb on + strand, within Psyr_0006 at 8.098 kb on + strand, within Psyr_0006 at 8.098 kb on + strand, within Psyr_0006 at 8.099 kb on - strand, within Psyr_0006 at 8.099 kb on - strand, within Psyr_0006 at 8.099 kb on - strand, within Psyr_0006 at 8.099 kb on - strand, within Psyr_0006 at 8.099 kb on - strand, within Psyr_0006 at 8.099 kb on - strand, within Psyr_0006 at 8.165 kb on - strand at 8.165 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 6,233 + +0.8 6,233 + +0.7 6,233 + -0.2 6,234 - -0.2 6,234 - +0.9 6,284 - -1.4 6,345 + -0.0 6,345 + +0.5 6,345 + -0.1 6,345 + +0.5 6,345 + -1.5 6,345 + -1.2 6,345 + -0.5 6,345 + +0.0 6,345 + -0.0 6,345 + -0.7 6,345 + -1.2 6,345 + -1.1 6,346 - -0.6 6,346 - -0.8 6,346 - -0.8 6,346 - -0.8 6,346 - -0.2 6,346 - +0.5 6,346 - -0.3 6,346 - -0.5 6,346 - +0.3 6,346 - +0.1 6,346 - -1.2 6,415 + Psyr_0005 0.15 +0.7 6,415 + Psyr_0005 0.15 -0.6 6,415 + Psyr_0005 0.15 -0.6 6,415 + Psyr_0005 0.15 -0.5 6,416 - Psyr_0005 0.15 +0.5 6,416 - Psyr_0005 0.15 -0.5 6,416 - Psyr_0005 0.15 -0.8 6,416 - Psyr_0005 0.15 -0.5 6,419 + Psyr_0005 0.15 -0.9 6,419 + Psyr_0005 0.15 +1.6 6,420 - Psyr_0005 0.16 -1.2 6,420 - Psyr_0005 0.16 -1.4 6,420 - Psyr_0005 0.16 -0.1 6,420 - Psyr_0005 0.16 +1.0 6,420 - Psyr_0005 0.16 +0.5 6,420 - Psyr_0005 0.16 -2.7 6,476 + Psyr_0005 0.22 -0.7 6,477 - Psyr_0005 0.22 -0.1 6,520 + Psyr_0005 0.27 +0.7 6,520 + Psyr_0005 0.27 +0.7 6,521 - Psyr_0005 0.27 -0.5 6,521 - Psyr_0005 0.27 -0.4 6,521 - Psyr_0005 0.27 +0.6 6,521 - Psyr_0005 0.27 -2.5 6,529 + Psyr_0005 0.28 +0.1 6,530 - Psyr_0005 0.28 -0.5 6,530 - Psyr_0005 0.28 -0.6 6,583 + Psyr_0005 0.34 +1.9 6,584 - Psyr_0005 0.34 -1.7 6,658 + Psyr_0005 0.42 +0.0 6,658 + Psyr_0005 0.42 -1.2 6,658 + Psyr_0005 0.42 +0.4 6,658 + Psyr_0005 0.42 +0.0 6,658 + Psyr_0005 0.42 -0.6 6,659 - Psyr_0005 0.42 -1.0 6,659 - Psyr_0005 0.42 +0.9 6,659 - Psyr_0005 0.42 -0.7 6,659 - Psyr_0005 0.42 -0.7 6,659 - Psyr_0005 0.42 +0.0 6,659 - Psyr_0005 0.42 -0.4 6,659 - Psyr_0005 0.42 +0.9 6,659 - Psyr_0005 0.42 +0.7 6,659 - Psyr_0005 0.42 -0.2 6,721 + Psyr_0005 0.49 -0.7 6,721 + Psyr_0005 0.49 -0.2 6,740 - Psyr_0005 0.52 -0.3 6,740 - Psyr_0005 0.52 +0.2 6,740 - Psyr_0005 0.52 -0.7 6,770 + Psyr_0005 0.55 -0.5 6,770 + Psyr_0005 0.55 +0.2 6,770 + Psyr_0005 0.55 -0.0 6,770 + Psyr_0005 0.55 -0.4 6,770 + Psyr_0005 0.55 -0.1 6,770 + Psyr_0005 0.55 -0.2 6,770 + Psyr_0005 0.55 +0.4 6,771 - Psyr_0005 0.55 +0.5 6,771 - Psyr_0005 0.55 +1.3 6,771 - Psyr_0005 0.55 +0.0 6,771 - Psyr_0005 0.55 -0.9 6,783 + Psyr_0005 0.56 -0.2 6,900 + Psyr_0005 0.70 -0.1 6,901 - Psyr_0005 0.70 -1.3 6,901 - Psyr_0005 0.70 -1.2 6,937 + Psyr_0005 0.74 +0.1 6,937 + Psyr_0005 0.74 +0.1 6,937 + Psyr_0005 0.74 +0.6 6,937 + Psyr_0005 0.74 -1.4 6,938 - Psyr_0005 0.74 +0.9 6,938 - Psyr_0005 0.74 +1.1 6,938 - Psyr_0005 0.74 +0.1 6,938 - Psyr_0005 0.74 -0.4 7,001 - Psyr_0005 0.81 +0.3 7,030 + Psyr_0005 0.84 -0.2 7,031 - Psyr_0005 0.84 -0.2 7,070 - Psyr_0005 0.89 +1.2 7,125 - -0.3 7,169 + -0.7 7,169 + +0.1 7,170 - -2.7 7,170 - +0.3 7,170 - -1.4 7,170 - -0.3 7,188 + +0.2 7,189 - -0.4 7,246 + +0.4 7,247 - -0.1 7,247 - +1.2 7,283 - -0.1 7,317 + Psyr_0006 0.12 -0.5 7,317 + Psyr_0006 0.12 -0.5 7,317 + Psyr_0006 0.12 -1.0 7,317 + +0.9 7,317 + Psyr_0006 0.12 +0.1 7,317 + Psyr_0006 0.12 -1.1 7,317 + Psyr_0006 0.12 -0.0 7,318 - Psyr_0006 0.12 +0.7 7,318 - Psyr_0006 0.12 -1.5 7,318 - Psyr_0006 0.12 -0.2 7,318 - Psyr_0006 0.12 -1.2 7,318 - Psyr_0006 0.12 +0.2 7,321 + Psyr_0006 0.12 -0.7 7,322 - Psyr_0006 0.12 -0.1 7,322 - Psyr_0006 0.12 +0.4 7,322 - Psyr_0006 0.12 -0.2 7,535 + Psyr_0006 0.33 -1.1 7,536 + Psyr_0006 0.33 +0.0 7,536 + Psyr_0006 0.33 +2.9 7,536 + Psyr_0006 0.33 -1.7 7,536 + Psyr_0006 0.33 -0.2 7,536 + Psyr_0006 0.33 +0.4 7,536 + Psyr_0006 0.33 -0.2 7,536 + Psyr_0006 0.33 -1.2 7,536 + Psyr_0006 0.33 -0.6 7,536 + Psyr_0006 0.33 +0.6 7,537 - Psyr_0006 0.33 +1.0 7,537 - Psyr_0006 0.33 -1.1 7,537 - Psyr_0006 0.33 -2.8 7,537 - Psyr_0006 0.33 -0.5 7,537 - Psyr_0006 0.33 -0.2 7,537 - Psyr_0006 0.33 -0.9 7,537 - Psyr_0006 0.33 +0.7 7,537 - Psyr_0006 0.33 -1.1 7,537 - Psyr_0006 0.33 +1.9 7,537 - Psyr_0006 0.33 +0.6 7,537 - Psyr_0006 0.33 -0.1 7,537 - Psyr_0006 0.33 -1.3 7,550 - Psyr_0006 0.34 +0.4 7,550 - Psyr_0006 0.34 -0.4 7,628 + Psyr_0006 0.42 -0.5 7,628 + Psyr_0006 0.42 +1.2 7,628 + Psyr_0006 0.42 -0.4 7,628 + Psyr_0006 0.42 -0.8 7,628 + Psyr_0006 0.42 -0.6 7,628 + Psyr_0006 0.42 -0.4 7,628 + Psyr_0006 0.42 -1.4 7,628 + Psyr_0006 0.42 -1.9 7,629 - Psyr_0006 0.42 -0.7 7,629 - Psyr_0006 0.42 -2.2 7,629 - Psyr_0006 0.42 -0.4 7,629 - Psyr_0006 0.42 +0.6 7,629 - Psyr_0006 0.42 -3.7 7,629 - Psyr_0006 0.42 -2.1 7,629 - Psyr_0006 0.42 -1.5 7,629 - Psyr_0006 0.42 -1.1 7,629 - Psyr_0006 0.42 +0.3 7,676 + Psyr_0006 0.46 -0.9 7,676 + Psyr_0006 0.46 +0.6 7,676 + Psyr_0006 0.46 -0.8 7,677 - Psyr_0006 0.46 -0.7 7,710 - Psyr_0006 0.50 -0.3 7,749 - Psyr_0006 0.53 -0.1 7,849 - Psyr_0006 0.63 -0.5 7,849 - Psyr_0006 0.63 -1.6 7,963 - Psyr_0006 0.74 -1.3 7,970 + Psyr_0006 0.75 -1.8 7,970 + Psyr_0006 0.75 -1.1 8,098 + Psyr_0006 0.87 -0.3 8,098 + Psyr_0006 0.87 -0.4 8,098 + Psyr_0006 0.87 -0.1 8,099 - Psyr_0006 0.87 -0.7 8,099 - Psyr_0006 0.87 -0.9 8,099 - Psyr_0006 0.87 -0.9 8,099 - Psyr_0006 0.87 +2.5 8,099 - Psyr_0006 0.87 +0.9 8,099 - Psyr_0006 0.87 -0.7 8,165 - +0.3 8,165 - -0.9
Or see this region's nucleotide sequence