Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_91 and Pf1N1B4_92 are separated by 132 nucleotides Pf1N1B4_92 and Pf1N1B4_93 are separated by 232 nucleotides
Pf1N1B4_91 - monooxygenase, FAD-binding, at 95,202 to 96,731
_91
Pf1N1B4_92 - Porphobilinogen synthase (EC 4.2.1.24), at 96,864 to 97,838
_92
Pf1N1B4_93 - Phenazine biosynthesis protein PhzF like, at 98,071 to 98,913
_93
Position (kb)
96
97
98 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 95.998 kb on - strand, within Pf1N1B4_91 at 95.998 kb on - strand, within Pf1N1B4_91 at 96.151 kb on + strand, within Pf1N1B4_91 at 96.151 kb on + strand, within Pf1N1B4_91 at 96.186 kb on + strand, within Pf1N1B4_91 at 96.187 kb on - strand, within Pf1N1B4_91 at 96.187 kb on - strand, within Pf1N1B4_91 at 96.382 kb on - strand, within Pf1N1B4_91 at 96.510 kb on + strand, within Pf1N1B4_91 at 96.510 kb on + strand, within Pf1N1B4_91 at 96.511 kb on - strand, within Pf1N1B4_91 at 96.624 kb on + strand at 96.624 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.664 kb on - strand at 96.865 kb on + strand at 96.866 kb on - strand at 96.866 kb on - strand at 96.866 kb on - strand at 96.866 kb on - strand at 96.898 kb on + strand at 96.898 kb on + strand at 96.899 kb on - strand at 97.006 kb on + strand, within Pf1N1B4_92 at 97.025 kb on - strand, within Pf1N1B4_92 at 97.178 kb on - strand, within Pf1N1B4_92 at 97.178 kb on - strand, within Pf1N1B4_92 at 97.462 kb on + strand, within Pf1N1B4_92 at 97.559 kb on + strand, within Pf1N1B4_92 at 97.631 kb on - strand, within Pf1N1B4_92 at 97.890 kb on + strand at 97.891 kb on - strand at 98.084 kb on - strand at 98.094 kb on + strand at 98.095 kb on - strand at 98.095 kb on - strand at 98.095 kb on - strand at 98.095 kb on - strand at 98.095 kb on - strand at 98.095 kb on - strand at 98.192 kb on + strand, within Pf1N1B4_93 at 98.192 kb on + strand, within Pf1N1B4_93 at 98.192 kb on + strand, within Pf1N1B4_93 at 98.193 kb on - strand, within Pf1N1B4_93 at 98.193 kb on - strand, within Pf1N1B4_93 at 98.193 kb on - strand, within Pf1N1B4_93
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 95,998 - Pf1N1B4_91 0.52 -1.7 95,998 - Pf1N1B4_91 0.52 +2.3 96,151 + Pf1N1B4_91 0.62 -0.3 96,151 + Pf1N1B4_91 0.62 -1.2 96,186 + Pf1N1B4_91 0.64 -0.2 96,187 - Pf1N1B4_91 0.64 -0.2 96,187 - Pf1N1B4_91 0.64 -0.2 96,382 - Pf1N1B4_91 0.77 +1.1 96,510 + Pf1N1B4_91 0.85 -0.7 96,510 + Pf1N1B4_91 0.85 -1.7 96,511 - Pf1N1B4_91 0.86 -0.8 96,624 + +2.7 96,624 + -0.3 96,645 + +0.1 96,645 + +1.7 96,645 + +1.3 96,645 + -0.4 96,645 + -2.0 96,645 + -0.1 96,645 + +0.3 96,645 + +2.1 96,645 + -0.3 96,645 + +0.6 96,646 - -0.5 96,646 - -0.9 96,646 - -0.6 96,646 - -0.7 96,646 - -0.2 96,646 - +1.1 96,646 - -0.5 96,664 - -1.3 96,865 + -1.4 96,866 - -0.4 96,866 - +1.8 96,866 - +0.5 96,866 - -0.5 96,898 + -0.9 96,898 + +3.4 96,899 - +0.0 97,006 + Pf1N1B4_92 0.15 -1.9 97,025 - Pf1N1B4_92 0.17 +0.8 97,178 - Pf1N1B4_92 0.32 -0.8 97,178 - Pf1N1B4_92 0.32 -1.2 97,462 + Pf1N1B4_92 0.61 +0.1 97,559 + Pf1N1B4_92 0.71 -0.7 97,631 - Pf1N1B4_92 0.79 -1.7 97,890 + -0.9 97,891 - -0.9 98,084 - -1.5 98,094 + -0.2 98,095 - -0.2 98,095 - +1.4 98,095 - -0.5 98,095 - -0.8 98,095 - -1.6 98,095 - -0.2 98,192 + Pf1N1B4_93 0.14 +0.1 98,192 + Pf1N1B4_93 0.14 +0.5 98,192 + Pf1N1B4_93 0.14 -1.5 98,193 - Pf1N1B4_93 0.14 +0.7 98,193 - Pf1N1B4_93 0.14 +0.3 98,193 - Pf1N1B4_93 0.14 +0.4
Or see this region's nucleotide sequence