Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6037 and Pf1N1B4_6038 are separated by 213 nucleotides Pf1N1B4_6038 and Pf1N1B4_6039 are separated by 4 nucleotides Pf1N1B4_6039 and Pf1N1B4_6040 are separated by 74 nucleotides Pf1N1B4_6040 and Pf1N1B4_6041 are separated by 249 nucleotides
Pf1N1B4_6037 - L-asparaginase (EC 3.5.1.1), at 501,468 to 502,556
_6037
Pf1N1B4_6038 - FIG00953874: hypothetical protein, at 502,770 to 503,009
_6038
Pf1N1B4_6039 - Endonuclease I precursor (EC 3.1.21.1), at 503,014 to 503,703
_6039
Pf1N1B4_6040 - FIG00956858: hypothetical protein, at 503,778 to 504,143
_6040
Pf1N1B4_6041 - Carbon storage regulator, at 504,393 to 504,587
_6041
Position (kb)
503
504 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 502.230 kb on + strand, within Pf1N1B4_6037 at 502.255 kb on - strand at 502.366 kb on + strand, within Pf1N1B4_6037 at 502.366 kb on + strand, within Pf1N1B4_6037 at 502.408 kb on + strand at 502.440 kb on + strand, within Pf1N1B4_6037 at 502.440 kb on + strand, within Pf1N1B4_6037 at 502.441 kb on - strand, within Pf1N1B4_6037 at 502.551 kb on + strand at 502.551 kb on + strand at 502.551 kb on + strand at 502.552 kb on - strand at 502.660 kb on - strand at 502.705 kb on - strand at 502.734 kb on + strand at 502.764 kb on - strand at 502.796 kb on + strand at 502.796 kb on + strand, within Pf1N1B4_6038 at 502.800 kb on + strand, within Pf1N1B4_6038 at 502.800 kb on + strand, within Pf1N1B4_6038 at 502.800 kb on + strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 503.036 kb on + strand at 503.119 kb on + strand, within Pf1N1B4_6039 at 503.144 kb on - strand at 503.158 kb on + strand, within Pf1N1B4_6039 at 503.158 kb on + strand, within Pf1N1B4_6039 at 503.159 kb on - strand at 503.195 kb on - strand at 503.195 kb on - strand, within Pf1N1B4_6039 at 503.329 kb on + strand, within Pf1N1B4_6039 at 503.330 kb on - strand, within Pf1N1B4_6039 at 503.330 kb on - strand at 503.437 kb on + strand, within Pf1N1B4_6039 at 503.521 kb on + strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.527 kb on + strand, within Pf1N1B4_6039 at 503.528 kb on - strand, within Pf1N1B4_6039 at 503.528 kb on - strand, within Pf1N1B4_6039 at 503.528 kb on - strand, within Pf1N1B4_6039 at 503.535 kb on + strand, within Pf1N1B4_6039 at 503.535 kb on + strand, within Pf1N1B4_6039 at 503.578 kb on + strand, within Pf1N1B4_6039 at 503.578 kb on + strand, within Pf1N1B4_6039 at 503.579 kb on - strand at 503.579 kb on - strand, within Pf1N1B4_6039 at 503.592 kb on + strand, within Pf1N1B4_6039 at 503.599 kb on + strand, within Pf1N1B4_6039 at 503.600 kb on - strand, within Pf1N1B4_6039 at 503.889 kb on - strand, within Pf1N1B4_6040 at 503.889 kb on - strand, within Pf1N1B4_6040 at 503.948 kb on + strand at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 504.115 kb on - strand at 504.115 kb on - strand at 504.251 kb on - strand at 504.251 kb on - strand at 504.252 kb on + strand at 504.252 kb on + strand at 504.252 kb on + strand at 504.252 kb on + strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.257 kb on - strand at 504.271 kb on - strand at 504.271 kb on - strand at 504.324 kb on - strand at 504.324 kb on - strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.329 kb on - strand at 504.329 kb on - strand at 504.329 kb on - strand at 504.329 kb on - strand at 504.329 kb on - strand at 504.329 kb on - strand at 504.331 kb on - strand at 504.331 kb on - strand at 504.334 kb on + strand at 504.334 kb on + strand at 504.334 kb on + strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.369 kb on + strand at 504.370 kb on - strand at 504.400 kb on + strand at 504.427 kb on + strand, within Pf1N1B4_6041 at 504.428 kb on - strand, within Pf1N1B4_6041 at 504.446 kb on + strand, within Pf1N1B4_6041 at 504.447 kb on - strand, within Pf1N1B4_6041
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 502,230 + Pf1N1B4_6037 0.70 -0.1 502,255 - -1.1 502,366 + Pf1N1B4_6037 0.82 -0.4 502,366 + Pf1N1B4_6037 0.82 -1.3 502,408 + -0.1 502,440 + Pf1N1B4_6037 0.89 -0.1 502,440 + Pf1N1B4_6037 0.89 -1.1 502,441 - Pf1N1B4_6037 0.89 -1.1 502,551 + -2.1 502,551 + -0.4 502,551 + +0.9 502,552 - +0.4 502,660 - +0.5 502,705 - -0.1 502,734 + +0.4 502,764 - -2.4 502,796 + -0.1 502,796 + Pf1N1B4_6038 0.11 -0.1 502,800 + Pf1N1B4_6038 0.12 +0.6 502,800 + Pf1N1B4_6038 0.12 -0.9 502,800 + Pf1N1B4_6038 0.12 -0.3 502,801 - Pf1N1B4_6038 0.13 +0.5 502,801 - Pf1N1B4_6038 0.13 -2.6 502,801 - Pf1N1B4_6038 0.13 -1.3 502,801 - Pf1N1B4_6038 0.13 +0.3 503,036 + -2.3 503,119 + Pf1N1B4_6039 0.15 +2.5 503,144 - -0.1 503,158 + Pf1N1B4_6039 0.21 -2.4 503,158 + Pf1N1B4_6039 0.21 -0.6 503,159 - +2.9 503,195 - +0.3 503,195 - Pf1N1B4_6039 0.26 -1.8 503,329 + Pf1N1B4_6039 0.46 -0.5 503,330 - Pf1N1B4_6039 0.46 -2.1 503,330 - +2.5 503,437 + Pf1N1B4_6039 0.61 -0.8 503,521 + Pf1N1B4_6039 0.73 -0.8 503,522 - Pf1N1B4_6039 0.74 -1.6 503,522 - Pf1N1B4_6039 0.74 -1.7 503,522 - Pf1N1B4_6039 0.74 -2.7 503,522 - Pf1N1B4_6039 0.74 -2.1 503,527 + Pf1N1B4_6039 0.74 -0.1 503,528 - Pf1N1B4_6039 0.74 -0.1 503,528 - Pf1N1B4_6039 0.74 -0.9 503,528 - Pf1N1B4_6039 0.74 -0.1 503,535 + Pf1N1B4_6039 0.76 -0.2 503,535 + Pf1N1B4_6039 0.76 -1.9 503,578 + Pf1N1B4_6039 0.82 -0.6 503,578 + Pf1N1B4_6039 0.82 -0.8 503,579 - +0.5 503,579 - Pf1N1B4_6039 0.82 -1.2 503,592 + Pf1N1B4_6039 0.84 -0.1 503,599 + Pf1N1B4_6039 0.85 -0.3 503,600 - Pf1N1B4_6039 0.85 -0.0 503,889 - Pf1N1B4_6040 0.30 -0.1 503,889 - Pf1N1B4_6040 0.30 -0.7 503,948 + -0.1 503,949 - Pf1N1B4_6040 0.47 -1.7 503,949 - -0.7 503,949 - Pf1N1B4_6040 0.47 -1.7 503,949 - Pf1N1B4_6040 0.47 -0.6 503,949 - Pf1N1B4_6040 0.47 -1.3 503,949 - Pf1N1B4_6040 0.47 -0.1 503,949 - Pf1N1B4_6040 0.47 +0.1 503,949 - Pf1N1B4_6040 0.47 -1.1 503,949 - Pf1N1B4_6040 0.47 +0.5 503,949 - Pf1N1B4_6040 0.47 -1.4 504,115 - -0.7 504,115 - -0.4 504,251 - -0.1 504,251 - -1.1 504,252 + -0.1 504,252 + -0.7 504,252 + -0.9 504,252 + -0.8 504,253 - +0.9 504,253 - -0.1 504,253 - +0.0 504,253 - -1.8 504,253 - +0.9 504,253 - +0.9 504,253 - -1.1 504,253 - -0.7 504,257 - -1.1 504,271 - -1.9 504,271 - +0.3 504,324 - +0.3 504,324 - -0.3 504,328 + -0.4 504,328 + +0.5 504,328 + +0.9 504,328 + -0.5 504,328 + -0.1 504,328 + +0.1 504,328 + -0.2 504,328 + -1.7 504,329 - -1.1 504,329 - +0.9 504,329 - -0.3 504,329 - +1.2 504,329 - -0.4 504,329 - -0.4 504,331 - -3.8 504,331 - -0.4 504,334 + -0.7 504,334 + -0.4 504,334 + -2.0 504,335 - -0.8 504,335 - -0.1 504,335 - +0.2 504,335 - -0.1 504,335 - -1.7 504,335 - +0.9 504,335 - -0.3 504,335 - -0.5 504,369 + -0.5 504,370 - -0.6 504,400 + -0.7 504,427 + Pf1N1B4_6041 0.17 +0.3 504,428 - Pf1N1B4_6041 0.18 -0.2 504,446 + Pf1N1B4_6041 0.27 -1.9 504,447 - Pf1N1B4_6041 0.28 -0.7
Or see this region's nucleotide sequence