Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3011 and Pf1N1B4_3012 are separated by 165 nucleotides Pf1N1B4_3012 and Pf1N1B4_3013 are separated by 79 nucleotides
Pf1N1B4_3011 - Tyrosine recombinase XerD, at 3,385,335 to 3,386,231
_3011
Pf1N1B4_3012 - Thiol:disulfide interchange protein DsbC, at 3,386,397 to 3,387,128
_3012
Pf1N1B4_3013 - Homoserine dehydrogenase (EC 1.1.1.3), at 3,387,208 to 3,388,566
_3013
Position (kb)
3386
3387
3388 Strain fitness (log2 ratio)
-1
0
1
2 at 3385.536 kb on + strand, within Pf1N1B4_3011 at 3385.536 kb on + strand, within Pf1N1B4_3011 at 3385.585 kb on - strand, within Pf1N1B4_3011 at 3385.591 kb on - strand, within Pf1N1B4_3011 at 3385.667 kb on + strand, within Pf1N1B4_3011 at 3385.668 kb on - strand, within Pf1N1B4_3011 at 3385.702 kb on + strand, within Pf1N1B4_3011 at 3385.912 kb on - strand, within Pf1N1B4_3011 at 3385.912 kb on - strand, within Pf1N1B4_3011 at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.170 kb on - strand at 3386.170 kb on - strand at 3386.170 kb on - strand at 3386.170 kb on - strand at 3386.263 kb on - strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.297 kb on + strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.298 kb on - strand at 3386.304 kb on + strand at 3386.556 kb on + strand, within Pf1N1B4_3012 at 3386.610 kb on + strand, within Pf1N1B4_3012 at 3386.610 kb on + strand, within Pf1N1B4_3012 at 3386.610 kb on + strand, within Pf1N1B4_3012 at 3386.853 kb on + strand, within Pf1N1B4_3012 at 3386.853 kb on + strand, within Pf1N1B4_3012 at 3386.853 kb on + strand, within Pf1N1B4_3012 at 3386.853 kb on + strand, within Pf1N1B4_3012 at 3387.038 kb on + strand, within Pf1N1B4_3012 at 3387.038 kb on + strand, within Pf1N1B4_3012 at 3387.207 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 3,385,536 + Pf1N1B4_3011 0.22 -0.3 3,385,536 + Pf1N1B4_3011 0.22 -1.3 3,385,585 - Pf1N1B4_3011 0.28 -0.6 3,385,591 - Pf1N1B4_3011 0.29 +1.9 3,385,667 + Pf1N1B4_3011 0.37 -0.0 3,385,668 - Pf1N1B4_3011 0.37 -0.2 3,385,702 + Pf1N1B4_3011 0.41 +0.6 3,385,912 - Pf1N1B4_3011 0.64 -0.2 3,385,912 - Pf1N1B4_3011 0.64 +2.0 3,386,169 + +0.1 3,386,169 + +0.5 3,386,169 + +0.6 3,386,169 + -0.4 3,386,169 + -0.4 3,386,170 - -0.9 3,386,170 - -0.9 3,386,170 - +0.8 3,386,170 - +1.1 3,386,263 - +0.4 3,386,297 + -0.8 3,386,297 + +1.0 3,386,297 + +0.2 3,386,297 + -0.5 3,386,297 + -0.2 3,386,297 + +0.1 3,386,297 + -0.0 3,386,297 + -1.4 3,386,297 + +0.8 3,386,297 + +0.4 3,386,297 + +0.7 3,386,297 + -1.1 3,386,297 + +0.7 3,386,297 + +0.2 3,386,297 + -1.5 3,386,297 + +0.9 3,386,297 + +0.1 3,386,298 - -0.1 3,386,298 - -0.5 3,386,298 - -0.8 3,386,298 - +0.2 3,386,298 - -0.7 3,386,298 - +0.2 3,386,298 - +0.0 3,386,298 - +0.8 3,386,298 - -1.2 3,386,298 - +0.2 3,386,304 + +1.4 3,386,556 + Pf1N1B4_3012 0.22 -1.3 3,386,610 + Pf1N1B4_3012 0.29 -0.9 3,386,610 + Pf1N1B4_3012 0.29 -0.6 3,386,610 + Pf1N1B4_3012 0.29 -0.6 3,386,853 + Pf1N1B4_3012 0.62 -1.6 3,386,853 + Pf1N1B4_3012 0.62 +0.4 3,386,853 + Pf1N1B4_3012 0.62 +1.4 3,386,853 + Pf1N1B4_3012 0.62 -0.1 3,387,038 + Pf1N1B4_3012 0.88 -1.4 3,387,038 + Pf1N1B4_3012 0.88 -0.3 3,387,207 + -0.4
Or see this region's nucleotide sequence