Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2189 and Pf1N1B4_2190 are separated by 179 nucleotides Pf1N1B4_2190 and Pf1N1B4_2191 are separated by 62 nucleotides Pf1N1B4_2191 and Pf1N1B4_2192 are separated by 192 nucleotides
Pf1N1B4_2189 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 2,501,816 to 2,502,367
_2189
Pf1N1B4_2190 - Regulator of sigma D, at 2,502,547 to 2,503,005
_2190
Pf1N1B4_2191 - Macrophage infectivity potentiator, at 2,503,068 to 2,503,733
_2191
Pf1N1B4_2192 - Twin-arginine translocation protein TatB, at 2,503,926 to 2,505,059
_2192
Position (kb)
2503
2504 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2502.148 kb on + strand, within Pf1N1B4_2189 at 2502.148 kb on + strand, within Pf1N1B4_2189 at 2502.149 kb on - strand, within Pf1N1B4_2189 at 2502.149 kb on - strand, within Pf1N1B4_2189 at 2502.149 kb on - strand, within Pf1N1B4_2189 at 2502.365 kb on + strand at 2502.375 kb on + strand at 2502.375 kb on + strand at 2502.375 kb on + strand at 2502.384 kb on + strand at 2502.385 kb on - strand at 2502.385 kb on - strand at 2502.385 kb on - strand at 2502.395 kb on + strand at 2502.395 kb on + strand at 2502.422 kb on + strand at 2502.450 kb on + strand at 2502.450 kb on + strand at 2502.450 kb on + strand at 2502.450 kb on + strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.510 kb on + strand at 2502.510 kb on + strand at 2502.511 kb on - strand at 2502.511 kb on - strand at 2502.511 kb on - strand at 2502.511 kb on - strand at 2502.643 kb on + strand, within Pf1N1B4_2190 at 2502.643 kb on + strand at 2502.643 kb on + strand, within Pf1N1B4_2190 at 2502.644 kb on - strand, within Pf1N1B4_2190 at 2502.644 kb on - strand, within Pf1N1B4_2190 at 2502.644 kb on - strand, within Pf1N1B4_2190 at 2502.724 kb on + strand, within Pf1N1B4_2190 at 2502.724 kb on + strand, within Pf1N1B4_2190 at 2502.724 kb on + strand, within Pf1N1B4_2190 at 2502.725 kb on - strand, within Pf1N1B4_2190 at 2502.725 kb on - strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.752 kb on - strand, within Pf1N1B4_2190 at 2502.752 kb on - strand, within Pf1N1B4_2190 at 2502.752 kb on - strand, within Pf1N1B4_2190 at 2502.789 kb on + strand, within Pf1N1B4_2190 at 2502.820 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2503.076 kb on + strand at 2503.076 kb on + strand at 2503.077 kb on - strand at 2503.151 kb on - strand, within Pf1N1B4_2191 at 2503.151 kb on - strand, within Pf1N1B4_2191 at 2503.235 kb on + strand, within Pf1N1B4_2191 at 2503.235 kb on + strand, within Pf1N1B4_2191 at 2503.235 kb on + strand, within Pf1N1B4_2191 at 2503.236 kb on - strand, within Pf1N1B4_2191 at 2503.236 kb on - strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.372 kb on - strand, within Pf1N1B4_2191 at 2503.372 kb on - strand, within Pf1N1B4_2191 at 2503.372 kb on - strand, within Pf1N1B4_2191 at 2503.549 kb on + strand, within Pf1N1B4_2191 at 2503.549 kb on + strand, within Pf1N1B4_2191 at 2503.550 kb on - strand at 2503.550 kb on - strand, within Pf1N1B4_2191 at 2503.550 kb on - strand, within Pf1N1B4_2191 at 2503.550 kb on - strand, within Pf1N1B4_2191 at 2503.639 kb on + strand, within Pf1N1B4_2191 at 2503.639 kb on + strand, within Pf1N1B4_2191 at 2503.639 kb on + strand, within Pf1N1B4_2191 at 2503.714 kb on - strand at 2503.764 kb on + strand at 2503.765 kb on - strand at 2503.765 kb on - strand at 2503.765 kb on - strand at 2503.765 kb on - strand at 2503.765 kb on - strand at 2503.823 kb on - strand at 2503.837 kb on + strand at 2503.837 kb on + strand at 2503.837 kb on + strand at 2503.837 kb on + strand at 2503.838 kb on - strand at 2503.888 kb on + strand at 2503.888 kb on + strand at 2503.888 kb on + strand at 2503.889 kb on - strand at 2503.891 kb on + strand at 2503.891 kb on + strand at 2503.891 kb on + strand at 2503.892 kb on - strand at 2503.892 kb on - strand at 2503.892 kb on - strand at 2503.892 kb on - strand at 2503.920 kb on + strand at 2503.920 kb on + strand at 2503.921 kb on - strand at 2503.921 kb on - strand at 2503.921 kb on - strand at 2503.949 kb on - strand at 2503.949 kb on - strand at 2503.949 kb on - strand at 2503.949 kb on - strand at 2503.949 kb on - strand at 2503.949 kb on - strand at 2503.952 kb on + strand at 2503.952 kb on + strand at 2503.952 kb on + strand at 2503.952 kb on + strand at 2503.953 kb on - strand at 2503.953 kb on - strand at 2503.953 kb on - strand at 2503.953 kb on - strand at 2503.953 kb on - strand at 2503.966 kb on + strand at 2503.966 kb on + strand at 2503.967 kb on - strand at 2503.967 kb on - strand at 2503.967 kb on - strand at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.227 kb on - strand, within Pf1N1B4_2192 at 2504.227 kb on - strand, within Pf1N1B4_2192 at 2504.227 kb on - strand, within Pf1N1B4_2192 at 2504.290 kb on + strand, within Pf1N1B4_2192 at 2504.290 kb on + strand, within Pf1N1B4_2192 at 2504.290 kb on + strand, within Pf1N1B4_2192 at 2504.290 kb on + strand, within Pf1N1B4_2192 at 2504.290 kb on + strand, within Pf1N1B4_2192 at 2504.291 kb on - strand, within Pf1N1B4_2192 at 2504.291 kb on - strand, within Pf1N1B4_2192 at 2504.291 kb on - strand, within Pf1N1B4_2192 at 2504.291 kb on - strand, within Pf1N1B4_2192 at 2504.562 kb on - strand, within Pf1N1B4_2192 at 2504.720 kb on + strand, within Pf1N1B4_2192 at 2504.721 kb on - strand, within Pf1N1B4_2192 at 2504.721 kb on - strand, within Pf1N1B4_2192
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 2,502,148 + Pf1N1B4_2189 0.60 +1.2 2,502,148 + Pf1N1B4_2189 0.60 -1.3 2,502,149 - Pf1N1B4_2189 0.60 -0.2 2,502,149 - Pf1N1B4_2189 0.60 +0.1 2,502,149 - Pf1N1B4_2189 0.60 -0.5 2,502,365 + +0.1 2,502,375 + +1.0 2,502,375 + +0.7 2,502,375 + -0.7 2,502,384 + -0.0 2,502,385 - -0.7 2,502,385 - -0.1 2,502,385 - -0.3 2,502,395 + -0.9 2,502,395 + -0.7 2,502,422 + -1.1 2,502,450 + -0.3 2,502,450 + -0.1 2,502,450 + -1.4 2,502,450 + +2.3 2,502,451 - +0.1 2,502,451 - +0.3 2,502,451 - -1.2 2,502,451 - +0.9 2,502,451 - -0.0 2,502,451 - +1.1 2,502,451 - +2.2 2,502,451 - -0.4 2,502,451 - +0.5 2,502,510 + +0.1 2,502,510 + +0.4 2,502,511 - +0.1 2,502,511 - -0.9 2,502,511 - +0.7 2,502,511 - +0.5 2,502,643 + Pf1N1B4_2190 0.21 +0.9 2,502,643 + +0.1 2,502,643 + Pf1N1B4_2190 0.21 -1.5 2,502,644 - Pf1N1B4_2190 0.21 -0.1 2,502,644 - Pf1N1B4_2190 0.21 -1.5 2,502,644 - Pf1N1B4_2190 0.21 -0.7 2,502,724 + Pf1N1B4_2190 0.39 -1.5 2,502,724 + Pf1N1B4_2190 0.39 +1.7 2,502,724 + Pf1N1B4_2190 0.39 +0.4 2,502,725 - Pf1N1B4_2190 0.39 -0.1 2,502,725 - Pf1N1B4_2190 0.39 -0.9 2,502,751 + Pf1N1B4_2190 0.44 +1.3 2,502,751 + Pf1N1B4_2190 0.44 -0.2 2,502,751 + Pf1N1B4_2190 0.44 +0.5 2,502,751 + Pf1N1B4_2190 0.44 -1.1 2,502,752 - Pf1N1B4_2190 0.45 -1.2 2,502,752 - Pf1N1B4_2190 0.45 -1.7 2,502,752 - Pf1N1B4_2190 0.45 +0.3 2,502,789 + Pf1N1B4_2190 0.53 -1.4 2,502,820 + Pf1N1B4_2190 0.59 +0.9 2,502,869 + Pf1N1B4_2190 0.70 -0.3 2,502,869 + Pf1N1B4_2190 0.70 -0.7 2,502,869 + Pf1N1B4_2190 0.70 -0.1 2,502,869 + Pf1N1B4_2190 0.70 -0.3 2,502,869 + Pf1N1B4_2190 0.70 -1.1 2,502,869 + Pf1N1B4_2190 0.70 +0.9 2,502,869 + Pf1N1B4_2190 0.70 +0.2 2,502,869 + Pf1N1B4_2190 0.70 -0.2 2,502,869 + Pf1N1B4_2190 0.70 -0.1 2,502,869 + Pf1N1B4_2190 0.70 -1.0 2,502,869 + Pf1N1B4_2190 0.70 +1.3 2,502,869 + Pf1N1B4_2190 0.70 -1.7 2,502,869 + Pf1N1B4_2190 0.70 -0.4 2,502,869 + Pf1N1B4_2190 0.70 +0.1 2,502,870 - Pf1N1B4_2190 0.70 +0.4 2,502,870 - Pf1N1B4_2190 0.70 +0.5 2,502,870 - Pf1N1B4_2190 0.70 +0.5 2,502,870 - Pf1N1B4_2190 0.70 +0.2 2,503,076 + -0.8 2,503,076 + +1.7 2,503,077 - -0.9 2,503,151 - Pf1N1B4_2191 0.12 +1.3 2,503,151 - Pf1N1B4_2191 0.12 +1.1 2,503,235 + Pf1N1B4_2191 0.25 -0.7 2,503,235 + Pf1N1B4_2191 0.25 -1.9 2,503,235 + Pf1N1B4_2191 0.25 +0.3 2,503,236 - Pf1N1B4_2191 0.25 -1.5 2,503,236 - Pf1N1B4_2191 0.25 -0.7 2,503,303 + Pf1N1B4_2191 0.35 +0.7 2,503,303 + Pf1N1B4_2191 0.35 -1.3 2,503,303 + Pf1N1B4_2191 0.35 -0.3 2,503,303 + Pf1N1B4_2191 0.35 -0.3 2,503,303 + Pf1N1B4_2191 0.35 -0.1 2,503,303 + Pf1N1B4_2191 0.35 +0.3 2,503,303 + Pf1N1B4_2191 0.35 -0.6 2,503,303 + Pf1N1B4_2191 0.35 -1.9 2,503,303 + Pf1N1B4_2191 0.35 +0.7 2,503,303 + Pf1N1B4_2191 0.35 -0.8 2,503,303 + Pf1N1B4_2191 0.35 -1.3 2,503,303 + Pf1N1B4_2191 0.35 +1.4 2,503,303 + Pf1N1B4_2191 0.35 -0.8 2,503,303 + Pf1N1B4_2191 0.35 -1.0 2,503,304 - Pf1N1B4_2191 0.35 -1.2 2,503,304 - Pf1N1B4_2191 0.35 +0.7 2,503,304 - Pf1N1B4_2191 0.35 -2.4 2,503,304 - Pf1N1B4_2191 0.35 -0.7 2,503,304 - Pf1N1B4_2191 0.35 -1.9 2,503,304 - Pf1N1B4_2191 0.35 -0.9 2,503,304 - Pf1N1B4_2191 0.35 -0.2 2,503,304 - Pf1N1B4_2191 0.35 +0.1 2,503,304 - Pf1N1B4_2191 0.35 -0.2 2,503,304 - Pf1N1B4_2191 0.35 -0.5 2,503,304 - Pf1N1B4_2191 0.35 +0.7 2,503,304 - Pf1N1B4_2191 0.35 -1.1 2,503,304 - Pf1N1B4_2191 0.35 +0.7 2,503,372 - Pf1N1B4_2191 0.46 -1.7 2,503,372 - Pf1N1B4_2191 0.46 +0.7 2,503,372 - Pf1N1B4_2191 0.46 +0.6 2,503,549 + Pf1N1B4_2191 0.72 +0.1 2,503,549 + Pf1N1B4_2191 0.72 +0.7 2,503,550 - +0.7 2,503,550 - Pf1N1B4_2191 0.72 -1.1 2,503,550 - Pf1N1B4_2191 0.72 +0.1 2,503,550 - Pf1N1B4_2191 0.72 +0.3 2,503,639 + Pf1N1B4_2191 0.86 -0.9 2,503,639 + Pf1N1B4_2191 0.86 +0.7 2,503,639 + Pf1N1B4_2191 0.86 -1.1 2,503,714 - +1.1 2,503,764 + -0.7 2,503,765 - +1.2 2,503,765 - +0.7 2,503,765 - -1.8 2,503,765 - +1.1 2,503,765 - -0.9 2,503,823 - +1.3 2,503,837 + -0.3 2,503,837 + -1.2 2,503,837 + +0.3 2,503,837 + +0.7 2,503,838 - -0.3 2,503,888 + +0.3 2,503,888 + -0.1 2,503,888 + +0.1 2,503,889 - +1.2 2,503,891 + +0.2 2,503,891 + +0.6 2,503,891 + +0.2 2,503,892 - -0.2 2,503,892 - -1.1 2,503,892 - +1.4 2,503,892 - -1.9 2,503,920 + -0.1 2,503,920 + +0.7 2,503,921 - -0.5 2,503,921 - -0.6 2,503,921 - -0.5 2,503,949 - +1.1 2,503,949 - +0.1 2,503,949 - -1.1 2,503,949 - -2.3 2,503,949 - -0.5 2,503,949 - +0.3 2,503,952 + +0.2 2,503,952 + +0.5 2,503,952 + -0.7 2,503,952 + -0.7 2,503,953 - -2.3 2,503,953 - -0.9 2,503,953 - -0.0 2,503,953 - -0.5 2,503,953 - -1.7 2,503,966 + +0.1 2,503,966 + +0.1 2,503,967 - -0.7 2,503,967 - +1.7 2,503,967 - +0.1 2,504,226 + Pf1N1B4_2192 0.26 -0.3 2,504,226 + Pf1N1B4_2192 0.26 -0.2 2,504,226 + Pf1N1B4_2192 0.26 +0.9 2,504,226 + Pf1N1B4_2192 0.26 -0.5 2,504,226 + Pf1N1B4_2192 0.26 +0.4 2,504,226 + Pf1N1B4_2192 0.26 -0.8 2,504,227 - Pf1N1B4_2192 0.27 +0.8 2,504,227 - Pf1N1B4_2192 0.27 -0.5 2,504,227 - Pf1N1B4_2192 0.27 +1.7 2,504,290 + Pf1N1B4_2192 0.32 +2.2 2,504,290 + Pf1N1B4_2192 0.32 -0.3 2,504,290 + Pf1N1B4_2192 0.32 +0.4 2,504,290 + Pf1N1B4_2192 0.32 -0.1 2,504,290 + Pf1N1B4_2192 0.32 -0.0 2,504,291 - Pf1N1B4_2192 0.32 -0.7 2,504,291 - Pf1N1B4_2192 0.32 +1.7 2,504,291 - Pf1N1B4_2192 0.32 -1.5 2,504,291 - Pf1N1B4_2192 0.32 -0.9 2,504,562 - Pf1N1B4_2192 0.56 +1.1 2,504,720 + Pf1N1B4_2192 0.70 -0.7 2,504,721 - Pf1N1B4_2192 0.70 +1.3 2,504,721 - Pf1N1B4_2192 0.70 -1.7
Or see this region's nucleotide sequence