Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1278 and Pf1N1B4_1279 are separated by 10 nucleotides Pf1N1B4_1279 and Pf1N1B4_1280 overlap by 4 nucleotides
Pf1N1B4_1278 - Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily, at 1,458,634 to 1,459,914
_1278
Pf1N1B4_1279 - Flp pilus assembly protein TadB, at 1,459,925 to 1,460,809
_1279
Pf1N1B4_1280 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 1,460,806 to 1,461,696
_1280
Position (kb)
1459
1460
1461 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1459.252 kb on - strand, within Pf1N1B4_1278 at 1459.415 kb on + strand, within Pf1N1B4_1278 at 1459.416 kb on - strand, within Pf1N1B4_1278 at 1459.777 kb on - strand, within Pf1N1B4_1278 at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.803 kb on + strand at 1459.809 kb on - strand at 1459.912 kb on + strand at 1459.912 kb on + strand at 1459.991 kb on + strand at 1459.992 kb on - strand at 1460.103 kb on + strand, within Pf1N1B4_1279 at 1460.104 kb on - strand, within Pf1N1B4_1279 at 1460.246 kb on + strand, within Pf1N1B4_1279 at 1460.247 kb on - strand, within Pf1N1B4_1279 at 1460.247 kb on - strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.805 kb on + strand at 1460.806 kb on - strand at 1460.813 kb on + strand at 1461.020 kb on + strand, within Pf1N1B4_1280 at 1461.020 kb on + strand, within Pf1N1B4_1280 at 1461.021 kb on - strand, within Pf1N1B4_1280 at 1461.089 kb on + strand, within Pf1N1B4_1280 at 1461.090 kb on - strand, within Pf1N1B4_1280 at 1461.327 kb on - strand, within Pf1N1B4_1280 at 1461.350 kb on + strand, within Pf1N1B4_1280 at 1461.350 kb on + strand, within Pf1N1B4_1280 at 1461.351 kb on - strand, within Pf1N1B4_1280 at 1461.351 kb on - strand, within Pf1N1B4_1280 at 1461.351 kb on - strand, within Pf1N1B4_1280 at 1461.383 kb on + strand, within Pf1N1B4_1280 at 1461.479 kb on + strand, within Pf1N1B4_1280 at 1461.479 kb on + strand, within Pf1N1B4_1280 at 1461.480 kb on - strand, within Pf1N1B4_1280 at 1461.480 kb on - strand, within Pf1N1B4_1280 at 1461.577 kb on + strand, within Pf1N1B4_1280 at 1461.577 kb on + strand at 1461.577 kb on + strand, within Pf1N1B4_1280 at 1461.577 kb on + strand, within Pf1N1B4_1280 at 1461.577 kb on + strand, within Pf1N1B4_1280 at 1461.578 kb on - strand, within Pf1N1B4_1280 at 1461.578 kb on - strand, within Pf1N1B4_1280 at 1461.578 kb on - strand, within Pf1N1B4_1280 at 1461.578 kb on - strand, within Pf1N1B4_1280 at 1461.578 kb on - strand, within Pf1N1B4_1280 at 1461.578 kb on - strand, within Pf1N1B4_1280 at 1461.717 kb on + strand at 1461.784 kb on + strand at 1461.784 kb on + strand at 1461.784 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 1,459,252 - Pf1N1B4_1278 0.48 +0.3 1,459,415 + Pf1N1B4_1278 0.61 +0.8 1,459,416 - Pf1N1B4_1278 0.61 -0.1 1,459,777 - Pf1N1B4_1278 0.89 +1.3 1,459,792 + -0.5 1,459,792 + +0.5 1,459,792 + +0.5 1,459,792 + -0.9 1,459,792 + +0.9 1,459,792 + -0.1 1,459,793 - +0.9 1,459,793 - +2.1 1,459,793 - -1.1 1,459,793 - +0.4 1,459,793 - -1.6 1,459,793 - +2.0 1,459,793 - +0.1 1,459,793 - +1.0 1,459,803 + -1.2 1,459,809 - +0.0 1,459,912 + -1.3 1,459,912 + +0.5 1,459,991 + -0.6 1,459,992 - +0.7 1,460,103 + Pf1N1B4_1279 0.20 -1.2 1,460,104 - Pf1N1B4_1279 0.20 -0.5 1,460,246 + Pf1N1B4_1279 0.36 +0.5 1,460,247 - Pf1N1B4_1279 0.36 +1.1 1,460,247 - Pf1N1B4_1279 0.36 -0.3 1,460,699 + Pf1N1B4_1279 0.87 -2.7 1,460,699 + Pf1N1B4_1279 0.87 -1.8 1,460,699 + Pf1N1B4_1279 0.87 +1.3 1,460,699 + Pf1N1B4_1279 0.87 -1.3 1,460,805 + -2.3 1,460,806 - +0.2 1,460,813 + -1.9 1,461,020 + Pf1N1B4_1280 0.24 -0.4 1,461,020 + Pf1N1B4_1280 0.24 +0.5 1,461,021 - Pf1N1B4_1280 0.24 -1.2 1,461,089 + Pf1N1B4_1280 0.32 +0.1 1,461,090 - Pf1N1B4_1280 0.32 -0.5 1,461,327 - Pf1N1B4_1280 0.58 -0.1 1,461,350 + Pf1N1B4_1280 0.61 -0.3 1,461,350 + Pf1N1B4_1280 0.61 +0.2 1,461,351 - Pf1N1B4_1280 0.61 -1.5 1,461,351 - Pf1N1B4_1280 0.61 -0.6 1,461,351 - Pf1N1B4_1280 0.61 -0.2 1,461,383 + Pf1N1B4_1280 0.65 -2.3 1,461,479 + Pf1N1B4_1280 0.76 +2.0 1,461,479 + Pf1N1B4_1280 0.76 -1.6 1,461,480 - Pf1N1B4_1280 0.76 -1.5 1,461,480 - Pf1N1B4_1280 0.76 +0.1 1,461,577 + Pf1N1B4_1280 0.87 +0.2 1,461,577 + -0.1 1,461,577 + Pf1N1B4_1280 0.87 +0.5 1,461,577 + Pf1N1B4_1280 0.87 +1.1 1,461,577 + Pf1N1B4_1280 0.87 -1.9 1,461,578 - Pf1N1B4_1280 0.87 +1.5 1,461,578 - Pf1N1B4_1280 0.87 +0.9 1,461,578 - Pf1N1B4_1280 0.87 +0.7 1,461,578 - Pf1N1B4_1280 0.87 -0.8 1,461,578 - Pf1N1B4_1280 0.87 +0.5 1,461,578 - Pf1N1B4_1280 0.87 -0.5 1,461,717 + +0.1 1,461,784 + -0.5 1,461,784 + -0.1 1,461,784 + +0.5
Or see this region's nucleotide sequence