Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1156 and Pf1N1B4_1157 are separated by 42 nucleotides Pf1N1B4_1157 and Pf1N1B4_1158 are separated by 12 nucleotides Pf1N1B4_1158 and Pf1N1B4_1159 are separated by 142 nucleotides Pf1N1B4_1159 and Pf1N1B4_1160 are separated by 3 nucleotides Pf1N1B4_1160 and Pf1N1B4_1161 are separated by 159 nucleotides
Pf1N1B4_1156 - Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), at 1,303,300 to 1,305,405
_1156
Pf1N1B4_1157 - hypothetical protein, at 1,305,448 to 1,305,588
_1157
Pf1N1B4_1158 - SSU ribosomal protein S15p (S13e), at 1,305,601 to 1,305,870
_1158
Pf1N1B4_1159 - tRNA pseudouridine synthase B (EC 4.2.1.70), at 1,306,013 to 1,306,930
_1159
Pf1N1B4_1160 - Ribosome-binding factor A, at 1,306,934 to 1,307,335
_1160
Pf1N1B4_1161 - Translation initiation factor 2, at 1,307,495 to 1,310,020
_1161
Position (kb)
1306
1307 Strain fitness (log2 ratio)
-1
0
1 at 1306.345 kb on - strand, within Pf1N1B4_1159 at 1306.396 kb on - strand, within Pf1N1B4_1159 at 1306.396 kb on - strand, within Pf1N1B4_1159 at 1306.396 kb on - strand, within Pf1N1B4_1159 at 1306.396 kb on - strand, within Pf1N1B4_1159 at 1306.396 kb on - strand, within Pf1N1B4_1159 at 1306.469 kb on - strand, within Pf1N1B4_1159 at 1306.497 kb on - strand, within Pf1N1B4_1159 at 1306.521 kb on + strand, within Pf1N1B4_1159 at 1306.554 kb on + strand, within Pf1N1B4_1159 at 1306.729 kb on - strand, within Pf1N1B4_1159 at 1306.912 kb on + strand at 1306.913 kb on - strand at 1306.935 kb on + strand at 1307.351 kb on - strand at 1307.351 kb on - strand at 1307.497 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 1,306,345 - Pf1N1B4_1159 0.36 -0.1 1,306,396 - Pf1N1B4_1159 0.42 -0.5 1,306,396 - Pf1N1B4_1159 0.42 +0.1 1,306,396 - Pf1N1B4_1159 0.42 -0.7 1,306,396 - Pf1N1B4_1159 0.42 -0.6 1,306,396 - Pf1N1B4_1159 0.42 +0.5 1,306,469 - Pf1N1B4_1159 0.50 -0.5 1,306,497 - Pf1N1B4_1159 0.53 -1.5 1,306,521 + Pf1N1B4_1159 0.55 -0.5 1,306,554 + Pf1N1B4_1159 0.59 -0.3 1,306,729 - Pf1N1B4_1159 0.78 +0.3 1,306,912 + -1.7 1,306,913 - -1.3 1,306,935 + -0.3 1,307,351 - -0.8 1,307,351 - -0.4 1,307,497 - -0.3
Or see this region's nucleotide sequence