Experiment: R2A with Thallium(I) acetate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14515 and CA265_RS14520 are separated by 615 nucleotides CA265_RS14520 and CA265_RS14525 are separated by 99 nucleotides CA265_RS14525 and CA265_RS14530 are separated by 49 nucleotides
CA265_RS14515: CA265_RS14515 - aminodeoxychorismate synthase component I, at 3,657,361 to 3,658,617
_RS14515
CA265_RS14520: CA265_RS14520 - peptidase M48, at 3,659,233 to 3,660,039
_RS14520
CA265_RS14525: CA265_RS14525 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 3,660,139 to 3,660,786
_RS14525
CA265_RS14530: CA265_RS14530 - nicotinate-nucleotide diphosphorylase (carboxylating), at 3,660,836 to 3,661,678
_RS14530
Position (kb)
3659
3660
3661 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3658.347 kb on + strand, within CA265_RS14515 at 3658.393 kb on + strand, within CA265_RS14515 at 3658.470 kb on - strand, within CA265_RS14515 at 3658.490 kb on - strand, within CA265_RS14515 at 3658.512 kb on - strand at 3658.615 kb on + strand at 3658.677 kb on - strand at 3658.679 kb on - strand at 3658.731 kb on - strand at 3658.731 kb on - strand at 3658.733 kb on - strand at 3658.852 kb on + strand at 3658.853 kb on - strand at 3658.855 kb on - strand at 3658.855 kb on - strand at 3658.908 kb on + strand at 3659.076 kb on + strand at 3659.091 kb on - strand at 3659.126 kb on + strand at 3659.227 kb on + strand at 3659.242 kb on - strand at 3659.246 kb on + strand at 3659.247 kb on - strand at 3659.293 kb on - strand at 3659.311 kb on - strand at 3659.637 kb on + strand, within CA265_RS14520 at 3659.638 kb on - strand, within CA265_RS14520 at 3659.706 kb on + strand, within CA265_RS14520 at 3659.735 kb on - strand, within CA265_RS14520 at 3659.756 kb on + strand, within CA265_RS14520 at 3659.756 kb on + strand, within CA265_RS14520 at 3659.778 kb on - strand, within CA265_RS14520 at 3659.781 kb on + strand, within CA265_RS14520 at 3659.782 kb on - strand, within CA265_RS14520 at 3659.836 kb on - strand, within CA265_RS14520 at 3659.836 kb on - strand, within CA265_RS14520 at 3659.836 kb on - strand, within CA265_RS14520 at 3659.867 kb on - strand, within CA265_RS14520 at 3659.958 kb on + strand, within CA265_RS14520 at 3660.052 kb on - strand at 3660.066 kb on + strand at 3660.098 kb on + strand at 3660.098 kb on + strand at 3660.105 kb on - strand at 3660.111 kb on - strand at 3660.111 kb on - strand at 3660.838 kb on - strand at 3660.905 kb on + strand at 3660.922 kb on - strand, within CA265_RS14530 at 3660.934 kb on - strand, within CA265_RS14530 at 3661.031 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Thallium(I) acetate 0.001 mg/ml remove 3,658,347 + CA265_RS14515 0.78 +0.1 3,658,393 + CA265_RS14515 0.82 +0.6 3,658,470 - CA265_RS14515 0.88 -0.4 3,658,490 - CA265_RS14515 0.90 +0.7 3,658,512 - -1.0 3,658,615 + -0.2 3,658,677 - -0.8 3,658,679 - +1.3 3,658,731 - +0.6 3,658,731 - -0.8 3,658,733 - +0.1 3,658,852 + +0.8 3,658,853 - +1.3 3,658,855 - +0.6 3,658,855 - -0.1 3,658,908 + +0.3 3,659,076 + -0.4 3,659,091 - -1.5 3,659,126 + +0.1 3,659,227 + -0.4 3,659,242 - -0.7 3,659,246 + +0.7 3,659,247 - +0.3 3,659,293 - +0.3 3,659,311 - -0.8 3,659,637 + CA265_RS14520 0.50 +0.1 3,659,638 - CA265_RS14520 0.50 -0.1 3,659,706 + CA265_RS14520 0.59 +1.2 3,659,735 - CA265_RS14520 0.62 +0.6 3,659,756 + CA265_RS14520 0.65 +1.9 3,659,756 + CA265_RS14520 0.65 +1.9 3,659,778 - CA265_RS14520 0.68 +0.5 3,659,781 + CA265_RS14520 0.68 +0.2 3,659,782 - CA265_RS14520 0.68 +0.8 3,659,836 - CA265_RS14520 0.75 +0.6 3,659,836 - CA265_RS14520 0.75 +0.2 3,659,836 - CA265_RS14520 0.75 -0.7 3,659,867 - CA265_RS14520 0.79 +0.1 3,659,958 + CA265_RS14520 0.90 -1.2 3,660,052 - -1.2 3,660,066 + +1.2 3,660,098 + +0.2 3,660,098 + -0.9 3,660,105 - +0.6 3,660,111 - +0.2 3,660,111 - -0.7 3,660,838 - -0.7 3,660,905 + +3.9 3,660,922 - CA265_RS14530 0.10 +1.1 3,660,934 - CA265_RS14530 0.12 +0.6 3,661,031 - +1.3
Or see this region's nucleotide sequence