Experiment: R2A with Thallium(I) acetate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13185 and CA265_RS13190 are separated by 14 nucleotides CA265_RS13190 and CA265_RS13195 are separated by 60 nucleotides CA265_RS13195 and CA265_RS13200 overlap by 4 nucleotides CA265_RS13200 and CA265_RS13205 are separated by 3 nucleotides
CA265_RS13185: CA265_RS13185 - phosphoglucosamine mutase, at 3,282,025 to 3,283,410
_RS13185
CA265_RS13190: CA265_RS13190 - hypothetical protein, at 3,283,425 to 3,283,688
_RS13190
CA265_RS13195: CA265_RS13195 - SAM-dependent methyltransferase, at 3,283,749 to 3,284,480
_RS13195
CA265_RS13200: CA265_RS13200 - phosphatase PAP2 family protein, at 3,284,477 to 3,285,040
_RS13200
CA265_RS13205: CA265_RS13205 - zinc/iron permease, at 3,285,044 to 3,285,760
_RS13205
Position (kb)
3283
3284
3285 Strain fitness (log2 ratio)
-1
0
1
2 at 3283.456 kb on + strand, within CA265_RS13190 at 3283.456 kb on + strand, within CA265_RS13190 at 3283.491 kb on + strand, within CA265_RS13190 at 3283.492 kb on - strand, within CA265_RS13190 at 3283.508 kb on + strand, within CA265_RS13190 at 3283.509 kb on - strand, within CA265_RS13190 at 3283.547 kb on - strand, within CA265_RS13190 at 3283.547 kb on - strand, within CA265_RS13190 at 3283.578 kb on - strand, within CA265_RS13190 at 3283.589 kb on + strand, within CA265_RS13190 at 3283.652 kb on + strand, within CA265_RS13190 at 3283.692 kb on + strand at 3283.704 kb on + strand at 3283.713 kb on - strand at 3283.714 kb on + strand at 3283.714 kb on + strand at 3283.791 kb on + strand at 3283.791 kb on + strand at 3284.056 kb on - strand, within CA265_RS13195 at 3284.085 kb on - strand, within CA265_RS13195 at 3284.415 kb on + strand at 3284.422 kb on + strand at 3284.442 kb on - strand at 3284.462 kb on - strand at 3284.481 kb on + strand at 3284.481 kb on + strand at 3284.482 kb on - strand at 3284.569 kb on - strand, within CA265_RS13200 at 3284.635 kb on + strand, within CA265_RS13200 at 3284.636 kb on - strand, within CA265_RS13200 at 3284.651 kb on + strand, within CA265_RS13200 at 3284.724 kb on + strand, within CA265_RS13200 at 3284.799 kb on + strand, within CA265_RS13200 at 3284.837 kb on - strand, within CA265_RS13200 at 3284.839 kb on - strand, within CA265_RS13200 at 3284.890 kb on + strand, within CA265_RS13200 at 3284.896 kb on + strand, within CA265_RS13200 at 3284.931 kb on + strand, within CA265_RS13200 at 3284.933 kb on + strand, within CA265_RS13200 at 3284.934 kb on - strand, within CA265_RS13200 at 3284.991 kb on + strand at 3284.992 kb on - strand at 3284.992 kb on - strand at 3284.998 kb on + strand at 3284.999 kb on - strand at 3285.067 kb on - strand at 3285.141 kb on + strand, within CA265_RS13205 at 3285.165 kb on - strand, within CA265_RS13205 at 3285.165 kb on - strand, within CA265_RS13205 at 3285.169 kb on - strand, within CA265_RS13205 at 3285.187 kb on - strand, within CA265_RS13205 at 3285.206 kb on + strand, within CA265_RS13205 at 3285.206 kb on + strand, within CA265_RS13205 at 3285.241 kb on + strand, within CA265_RS13205 at 3285.241 kb on + strand, within CA265_RS13205 at 3285.258 kb on + strand, within CA265_RS13205 at 3285.313 kb on + strand, within CA265_RS13205 at 3285.313 kb on + strand, within CA265_RS13205 at 3285.366 kb on + strand, within CA265_RS13205 at 3285.366 kb on + strand, within CA265_RS13205 at 3285.409 kb on + strand, within CA265_RS13205 at 3285.471 kb on + strand, within CA265_RS13205
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Thallium(I) acetate 0.001 mg/ml remove 3,283,456 + CA265_RS13190 0.12 +0.8 3,283,456 + CA265_RS13190 0.12 +0.9 3,283,491 + CA265_RS13190 0.25 +0.0 3,283,492 - CA265_RS13190 0.25 +0.5 3,283,508 + CA265_RS13190 0.31 +0.2 3,283,509 - CA265_RS13190 0.32 -0.5 3,283,547 - CA265_RS13190 0.46 -0.2 3,283,547 - CA265_RS13190 0.46 +0.2 3,283,578 - CA265_RS13190 0.58 -1.3 3,283,589 + CA265_RS13190 0.62 -0.5 3,283,652 + CA265_RS13190 0.86 -0.1 3,283,692 + +1.3 3,283,704 + -0.3 3,283,713 - +0.9 3,283,714 + -0.5 3,283,714 + -0.8 3,283,791 + -1.6 3,283,791 + -0.2 3,284,056 - CA265_RS13195 0.42 -0.5 3,284,085 - CA265_RS13195 0.46 +1.0 3,284,415 + +0.2 3,284,422 + -1.2 3,284,442 - +0.2 3,284,462 - +0.2 3,284,481 + +0.2 3,284,481 + -0.3 3,284,482 - -0.1 3,284,569 - CA265_RS13200 0.16 -0.8 3,284,635 + CA265_RS13200 0.28 -0.2 3,284,636 - CA265_RS13200 0.28 -0.7 3,284,651 + CA265_RS13200 0.31 -1.3 3,284,724 + CA265_RS13200 0.44 +1.1 3,284,799 + CA265_RS13200 0.57 +0.3 3,284,837 - CA265_RS13200 0.64 -0.5 3,284,839 - CA265_RS13200 0.64 +1.2 3,284,890 + CA265_RS13200 0.73 +0.6 3,284,896 + CA265_RS13200 0.74 +0.4 3,284,931 + CA265_RS13200 0.80 +1.5 3,284,933 + CA265_RS13200 0.81 +0.3 3,284,934 - CA265_RS13200 0.81 +0.1 3,284,991 + +0.2 3,284,992 - +0.8 3,284,992 - +0.7 3,284,998 + -0.2 3,284,999 - +1.3 3,285,067 - +0.3 3,285,141 + CA265_RS13205 0.14 -0.3 3,285,165 - CA265_RS13205 0.17 +1.2 3,285,165 - CA265_RS13205 0.17 -1.2 3,285,169 - CA265_RS13205 0.17 +0.2 3,285,187 - CA265_RS13205 0.20 +1.1 3,285,206 + CA265_RS13205 0.23 -0.4 3,285,206 + CA265_RS13205 0.23 +0.7 3,285,241 + CA265_RS13205 0.27 +1.9 3,285,241 + CA265_RS13205 0.27 +0.5 3,285,258 + CA265_RS13205 0.30 -0.8 3,285,313 + CA265_RS13205 0.38 -0.2 3,285,313 + CA265_RS13205 0.38 +1.1 3,285,366 + CA265_RS13205 0.45 -1.0 3,285,366 + CA265_RS13205 0.45 +0.5 3,285,409 + CA265_RS13205 0.51 +0.3 3,285,471 + CA265_RS13205 0.60 -0.7
Or see this region's nucleotide sequence