Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS37000 and RR42_RS37005 are separated by 310 nucleotides RR42_RS37005 and RR42_RS37010 are separated by 15 nucleotides
RR42_RS37000: RR42_RS37000 - PbsX family transcriptional regulator, at 3,755,464 to 3,757,923
_RS37000
RR42_RS37005: RR42_RS37005 - IclR family transcriptional regulator, at 3,758,234 to 3,759,097
_RS37005
RR42_RS37010: RR42_RS37010 - cyclase, at 3,759,113 to 3,760,168
_RS37010
Position (kb)
3758
3759
3760 Strain fitness (log2 ratio)
-1
0
1
2 at 3757.424 kb on + strand, within RR42_RS37000 at 3757.556 kb on + strand, within RR42_RS37000 at 3757.556 kb on + strand, within RR42_RS37000 at 3757.557 kb on - strand, within RR42_RS37000 at 3757.557 kb on - strand, within RR42_RS37000 at 3757.557 kb on - strand, within RR42_RS37000 at 3757.702 kb on + strand at 3757.702 kb on + strand at 3757.703 kb on - strand at 3757.706 kb on + strand at 3757.706 kb on + strand at 3757.706 kb on + strand at 3757.707 kb on - strand at 3757.707 kb on - strand at 3757.707 kb on - strand at 3757.718 kb on + strand at 3757.734 kb on - strand at 3757.734 kb on - strand at 3757.737 kb on + strand at 3757.737 kb on + strand at 3757.738 kb on - strand at 3758.177 kb on + strand at 3758.177 kb on + strand at 3758.178 kb on - strand at 3758.178 kb on - strand at 3758.179 kb on + strand at 3758.201 kb on - strand at 3758.248 kb on + strand at 3758.249 kb on - strand at 3758.302 kb on + strand at 3758.303 kb on - strand at 3758.609 kb on + strand, within RR42_RS37005 at 3758.609 kb on + strand, within RR42_RS37005 at 3758.610 kb on - strand, within RR42_RS37005 at 3759.095 kb on + strand at 3759.339 kb on + strand, within RR42_RS37010 at 3759.339 kb on + strand, within RR42_RS37010 at 3759.339 kb on + strand, within RR42_RS37010 at 3759.339 kb on + strand, within RR42_RS37010 at 3759.339 kb on + strand, within RR42_RS37010 at 3759.339 kb on + strand, within RR42_RS37010 at 3759.339 kb on + strand, within RR42_RS37010 at 3759.340 kb on - strand, within RR42_RS37010 at 3759.340 kb on - strand, within RR42_RS37010 at 3759.344 kb on + strand, within RR42_RS37010 at 3759.344 kb on + strand, within RR42_RS37010 at 3759.344 kb on + strand, within RR42_RS37010 at 3759.345 kb on - strand, within RR42_RS37010 at 3759.345 kb on - strand, within RR42_RS37010 at 3759.425 kb on + strand, within RR42_RS37010 at 3759.504 kb on - strand, within RR42_RS37010 at 3759.504 kb on - strand, within RR42_RS37010 at 3759.506 kb on + strand, within RR42_RS37010 at 3759.506 kb on + strand, within RR42_RS37010 at 3759.507 kb on - strand, within RR42_RS37010 at 3759.552 kb on - strand, within RR42_RS37010 at 3759.666 kb on + strand, within RR42_RS37010 at 3759.666 kb on + strand, within RR42_RS37010 at 3759.667 kb on + strand, within RR42_RS37010 at 3759.667 kb on - strand, within RR42_RS37010 at 3759.929 kb on + strand, within RR42_RS37010 at 3759.930 kb on - strand, within RR42_RS37010 at 3760.046 kb on + strand, within RR42_RS37010 at 3760.047 kb on - strand, within RR42_RS37010
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,757,424 + RR42_RS37000 0.80 +0.2 3,757,556 + RR42_RS37000 0.85 -0.3 3,757,556 + RR42_RS37000 0.85 -0.3 3,757,557 - RR42_RS37000 0.85 +1.0 3,757,557 - RR42_RS37000 0.85 +0.2 3,757,557 - RR42_RS37000 0.85 +0.2 3,757,702 + -0.6 3,757,702 + -0.3 3,757,703 - +0.8 3,757,706 + -0.6 3,757,706 + -1.2 3,757,706 + +1.6 3,757,707 - -0.4 3,757,707 - +0.8 3,757,707 - +0.3 3,757,718 + -0.5 3,757,734 - -1.3 3,757,734 - +0.2 3,757,737 + -0.9 3,757,737 + -0.6 3,757,738 - -0.9 3,758,177 + -0.5 3,758,177 + +0.0 3,758,178 - +0.7 3,758,178 - -1.1 3,758,179 + -0.6 3,758,201 - +0.1 3,758,248 + -1.1 3,758,249 - +1.6 3,758,302 + -0.2 3,758,303 - -0.1 3,758,609 + RR42_RS37005 0.43 -0.7 3,758,609 + RR42_RS37005 0.43 -0.6 3,758,610 - RR42_RS37005 0.44 -1.3 3,759,095 + +2.1 3,759,339 + RR42_RS37010 0.21 +0.6 3,759,339 + RR42_RS37010 0.21 -0.5 3,759,339 + RR42_RS37010 0.21 -0.7 3,759,339 + RR42_RS37010 0.21 +0.3 3,759,339 + RR42_RS37010 0.21 -0.8 3,759,339 + RR42_RS37010 0.21 +0.4 3,759,339 + RR42_RS37010 0.21 -0.7 3,759,340 - RR42_RS37010 0.21 +1.9 3,759,340 - RR42_RS37010 0.21 +0.4 3,759,344 + RR42_RS37010 0.22 -0.3 3,759,344 + RR42_RS37010 0.22 -0.1 3,759,344 + RR42_RS37010 0.22 -0.5 3,759,345 - RR42_RS37010 0.22 -1.3 3,759,345 - RR42_RS37010 0.22 +1.4 3,759,425 + RR42_RS37010 0.30 -0.5 3,759,504 - RR42_RS37010 0.37 -0.8 3,759,504 - RR42_RS37010 0.37 +0.0 3,759,506 + RR42_RS37010 0.37 -0.3 3,759,506 + RR42_RS37010 0.37 -0.4 3,759,507 - RR42_RS37010 0.37 +0.5 3,759,552 - RR42_RS37010 0.42 +1.4 3,759,666 + RR42_RS37010 0.52 +0.1 3,759,666 + RR42_RS37010 0.52 -0.6 3,759,667 + RR42_RS37010 0.52 +1.0 3,759,667 - RR42_RS37010 0.52 -0.2 3,759,929 + RR42_RS37010 0.77 -0.2 3,759,930 - RR42_RS37010 0.77 -0.2 3,760,046 + RR42_RS37010 0.88 -0.5 3,760,047 - RR42_RS37010 0.88 +0.3
Or see this region's nucleotide sequence