Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35835 and RR42_RS35840 are separated by 153 nucleotides RR42_RS35840 and RR42_RS35845 are separated by 45 nucleotides RR42_RS35845 and RR42_RS35850 are separated by 168 nucleotides
RR42_RS35835: RR42_RS35835 - chemotaxis protein, at 3,485,897 to 3,487,720
_RS35835
RR42_RS35840: RR42_RS35840 - chemotaxis protein, at 3,487,874 to 3,489,514
_RS35840
RR42_RS35845: RR42_RS35845 - chemotaxis protein CheW, at 3,489,560 to 3,490,036
_RS35845
RR42_RS35850: RR42_RS35850 - hypothetical protein, at 3,490,205 to 3,490,588
_RS35850
Position (kb)
3487
3488
3489
3490 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3487.066 kb on + strand, within RR42_RS35835 at 3487.067 kb on - strand, within RR42_RS35835 at 3487.171 kb on + strand, within RR42_RS35835 at 3487.171 kb on + strand, within RR42_RS35835 at 3487.171 kb on + strand, within RR42_RS35835 at 3487.264 kb on + strand, within RR42_RS35835 at 3487.323 kb on + strand, within RR42_RS35835 at 3487.323 kb on + strand, within RR42_RS35835 at 3487.323 kb on + strand, within RR42_RS35835 at 3487.323 kb on + strand, within RR42_RS35835 at 3487.323 kb on + strand, within RR42_RS35835 at 3487.323 kb on + strand, within RR42_RS35835 at 3487.323 kb on + strand, within RR42_RS35835 at 3487.323 kb on + strand, within RR42_RS35835 at 3487.323 kb on + strand, within RR42_RS35835 at 3487.324 kb on - strand, within RR42_RS35835 at 3487.324 kb on - strand, within RR42_RS35835 at 3487.324 kb on - strand, within RR42_RS35835 at 3487.324 kb on - strand, within RR42_RS35835 at 3487.324 kb on - strand, within RR42_RS35835 at 3487.324 kb on - strand, within RR42_RS35835 at 3487.324 kb on - strand, within RR42_RS35835 at 3487.324 kb on - strand, within RR42_RS35835 at 3487.421 kb on + strand, within RR42_RS35835 at 3487.422 kb on - strand, within RR42_RS35835 at 3487.422 kb on - strand, within RR42_RS35835 at 3487.527 kb on + strand, within RR42_RS35835 at 3487.585 kb on - strand at 3487.727 kb on + strand at 3487.727 kb on + strand at 3487.727 kb on + strand at 3487.728 kb on - strand at 3487.809 kb on + strand at 3487.810 kb on - strand at 3487.821 kb on + strand at 3487.822 kb on - strand at 3488.365 kb on + strand, within RR42_RS35840 at 3488.671 kb on - strand, within RR42_RS35840 at 3488.684 kb on + strand, within RR42_RS35840 at 3488.927 kb on - strand, within RR42_RS35840 at 3488.927 kb on - strand, within RR42_RS35840 at 3489.126 kb on + strand, within RR42_RS35840 at 3489.183 kb on + strand, within RR42_RS35840 at 3489.237 kb on + strand, within RR42_RS35840 at 3489.238 kb on - strand, within RR42_RS35840 at 3489.238 kb on - strand, within RR42_RS35840 at 3489.404 kb on - strand at 3489.404 kb on - strand at 3489.424 kb on + strand at 3489.424 kb on + strand at 3489.424 kb on + strand at 3489.425 kb on - strand at 3489.459 kb on + strand at 3489.459 kb on + strand at 3489.460 kb on - strand at 3489.481 kb on - strand at 3489.494 kb on - strand at 3489.505 kb on + strand at 3489.506 kb on - strand at 3489.506 kb on - strand at 3489.546 kb on + strand at 3489.606 kb on - strand at 3489.606 kb on - strand at 3489.669 kb on + strand, within RR42_RS35845 at 3489.669 kb on + strand, within RR42_RS35845 at 3489.798 kb on + strand, within RR42_RS35845 at 3489.798 kb on + strand, within RR42_RS35845 at 3489.798 kb on + strand, within RR42_RS35845 at 3489.799 kb on - strand, within RR42_RS35845 at 3489.807 kb on + strand, within RR42_RS35845 at 3489.807 kb on + strand, within RR42_RS35845 at 3489.807 kb on + strand, within RR42_RS35845 at 3489.807 kb on + strand, within RR42_RS35845 at 3489.807 kb on + strand, within RR42_RS35845 at 3489.807 kb on + strand, within RR42_RS35845 at 3489.807 kb on + strand, within RR42_RS35845 at 3489.807 kb on + strand, within RR42_RS35845 at 3489.808 kb on - strand, within RR42_RS35845 at 3489.808 kb on - strand, within RR42_RS35845 at 3489.808 kb on - strand, within RR42_RS35845 at 3489.808 kb on - strand, within RR42_RS35845 at 3489.808 kb on - strand, within RR42_RS35845 at 3489.808 kb on - strand, within RR42_RS35845 at 3489.808 kb on - strand, within RR42_RS35845 at 3489.891 kb on + strand, within RR42_RS35845 at 3489.891 kb on + strand, within RR42_RS35845 at 3489.892 kb on - strand, within RR42_RS35845 at 3489.892 kb on - strand, within RR42_RS35845 at 3489.927 kb on + strand, within RR42_RS35845 at 3489.967 kb on - strand, within RR42_RS35845 at 3489.967 kb on - strand, within RR42_RS35845 at 3490.145 kb on + strand at 3490.152 kb on + strand at 3490.159 kb on + strand at 3490.195 kb on - strand at 3490.207 kb on - strand at 3490.207 kb on - strand at 3490.227 kb on - strand at 3490.365 kb on + strand, within RR42_RS35850 at 3490.365 kb on + strand, within RR42_RS35850 at 3490.366 kb on - strand, within RR42_RS35850
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,487,066 + RR42_RS35835 0.64 -0.5 3,487,067 - RR42_RS35835 0.64 +0.2 3,487,171 + RR42_RS35835 0.70 +0.1 3,487,171 + RR42_RS35835 0.70 -1.1 3,487,171 + RR42_RS35835 0.70 +0.9 3,487,264 + RR42_RS35835 0.75 +0.1 3,487,323 + RR42_RS35835 0.78 -0.2 3,487,323 + RR42_RS35835 0.78 +0.1 3,487,323 + RR42_RS35835 0.78 +0.1 3,487,323 + RR42_RS35835 0.78 -0.8 3,487,323 + RR42_RS35835 0.78 -0.9 3,487,323 + RR42_RS35835 0.78 +0.1 3,487,323 + RR42_RS35835 0.78 +1.3 3,487,323 + RR42_RS35835 0.78 -0.2 3,487,323 + RR42_RS35835 0.78 -0.7 3,487,324 - RR42_RS35835 0.78 +0.3 3,487,324 - RR42_RS35835 0.78 -0.5 3,487,324 - RR42_RS35835 0.78 +0.4 3,487,324 - RR42_RS35835 0.78 +0.4 3,487,324 - RR42_RS35835 0.78 +0.0 3,487,324 - RR42_RS35835 0.78 +0.8 3,487,324 - RR42_RS35835 0.78 -0.2 3,487,324 - RR42_RS35835 0.78 -0.3 3,487,421 + RR42_RS35835 0.84 +0.3 3,487,422 - RR42_RS35835 0.84 -0.3 3,487,422 - RR42_RS35835 0.84 -0.8 3,487,527 + RR42_RS35835 0.89 -0.5 3,487,585 - +1.5 3,487,727 + -0.2 3,487,727 + +0.7 3,487,727 + +0.5 3,487,728 - -0.5 3,487,809 + +0.9 3,487,810 - -0.1 3,487,821 + +0.8 3,487,822 - -0.5 3,488,365 + RR42_RS35840 0.30 -0.3 3,488,671 - RR42_RS35840 0.49 +1.3 3,488,684 + RR42_RS35840 0.49 -0.9 3,488,927 - RR42_RS35840 0.64 +1.4 3,488,927 - RR42_RS35840 0.64 +0.3 3,489,126 + RR42_RS35840 0.76 -0.1 3,489,183 + RR42_RS35840 0.80 +3.1 3,489,237 + RR42_RS35840 0.83 +2.7 3,489,238 - RR42_RS35840 0.83 +1.0 3,489,238 - RR42_RS35840 0.83 -0.1 3,489,404 - -0.3 3,489,404 - -0.6 3,489,424 + -0.4 3,489,424 + -0.0 3,489,424 + +1.1 3,489,425 - -0.1 3,489,459 + -0.4 3,489,459 + +0.4 3,489,460 - +0.4 3,489,481 - -0.0 3,489,494 - +0.4 3,489,505 + +0.5 3,489,506 - -0.2 3,489,506 - -0.8 3,489,546 + +0.0 3,489,606 - -1.6 3,489,606 - -1.6 3,489,669 + RR42_RS35845 0.23 +0.7 3,489,669 + RR42_RS35845 0.23 -0.7 3,489,798 + RR42_RS35845 0.50 -0.5 3,489,798 + RR42_RS35845 0.50 -1.3 3,489,798 + RR42_RS35845 0.50 +0.3 3,489,799 - RR42_RS35845 0.50 -0.2 3,489,807 + RR42_RS35845 0.52 +0.4 3,489,807 + RR42_RS35845 0.52 +0.4 3,489,807 + RR42_RS35845 0.52 -0.5 3,489,807 + RR42_RS35845 0.52 -0.4 3,489,807 + RR42_RS35845 0.52 -0.5 3,489,807 + RR42_RS35845 0.52 +0.1 3,489,807 + RR42_RS35845 0.52 -1.0 3,489,807 + RR42_RS35845 0.52 -0.5 3,489,808 - RR42_RS35845 0.52 -0.1 3,489,808 - RR42_RS35845 0.52 -2.3 3,489,808 - RR42_RS35845 0.52 -1.7 3,489,808 - RR42_RS35845 0.52 -0.3 3,489,808 - RR42_RS35845 0.52 -0.2 3,489,808 - RR42_RS35845 0.52 -1.5 3,489,808 - RR42_RS35845 0.52 +0.2 3,489,891 + RR42_RS35845 0.69 +0.1 3,489,891 + RR42_RS35845 0.69 -2.3 3,489,892 - RR42_RS35845 0.70 +0.6 3,489,892 - RR42_RS35845 0.70 +0.3 3,489,927 + RR42_RS35845 0.77 +2.3 3,489,967 - RR42_RS35845 0.85 -0.8 3,489,967 - RR42_RS35845 0.85 -2.2 3,490,145 + -0.2 3,490,152 + +0.2 3,490,159 + -1.7 3,490,195 - +0.1 3,490,207 - -0.1 3,490,207 - +0.4 3,490,227 - +1.2 3,490,365 + RR42_RS35850 0.42 -0.8 3,490,365 + RR42_RS35850 0.42 -1.0 3,490,366 - RR42_RS35850 0.42 -0.9
Or see this region's nucleotide sequence