Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33730 and RR42_RS33735 are separated by 43 nucleotides RR42_RS33735 and RR42_RS33740 are separated by 3 nucleotides
RR42_RS33730: RR42_RS33730 - hypothetical protein, at 2,994,251 to 2,995,537
_RS33730
RR42_RS33735: RR42_RS33735 - pyruvate kinase, at 2,995,581 to 2,997,011
_RS33735
RR42_RS33740: RR42_RS33740 - hydroxypyruvate reductase, at 2,997,015 to 2,998,310
_RS33740
Position (kb)
2995
2996
2997
2998 Strain fitness (log2 ratio)
-1
0
1
2 at 2994.777 kb on + strand, within RR42_RS33730 at 2994.777 kb on + strand, within RR42_RS33730 at 2994.777 kb on + strand, within RR42_RS33730 at 2994.778 kb on - strand, within RR42_RS33730 at 2994.778 kb on - strand, within RR42_RS33730 at 2994.984 kb on - strand, within RR42_RS33730 at 2995.147 kb on - strand, within RR42_RS33730 at 2995.437 kb on + strand at 2995.438 kb on - strand at 2995.502 kb on + strand at 2995.503 kb on - strand at 2995.717 kb on + strand at 2995.718 kb on - strand at 2995.882 kb on + strand, within RR42_RS33735 at 2995.899 kb on + strand, within RR42_RS33735 at 2995.997 kb on - strand, within RR42_RS33735 at 2995.997 kb on - strand, within RR42_RS33735 at 2996.110 kb on + strand, within RR42_RS33735 at 2996.110 kb on + strand, within RR42_RS33735 at 2996.111 kb on - strand, within RR42_RS33735 at 2996.468 kb on - strand, within RR42_RS33735 at 2997.016 kb on + strand at 2997.075 kb on - strand at 2997.478 kb on - strand, within RR42_RS33740 at 2997.478 kb on - strand, within RR42_RS33740 at 2997.478 kb on - strand, within RR42_RS33740 at 2997.481 kb on + strand, within RR42_RS33740 at 2997.481 kb on + strand, within RR42_RS33740 at 2997.482 kb on - strand, within RR42_RS33740 at 2997.741 kb on + strand, within RR42_RS33740 at 2997.762 kb on - strand, within RR42_RS33740 at 2997.806 kb on + strand, within RR42_RS33740 at 2997.806 kb on + strand, within RR42_RS33740 at 2997.807 kb on - strand, within RR42_RS33740 at 2997.924 kb on - strand, within RR42_RS33740 at 2997.968 kb on - strand, within RR42_RS33740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,994,777 + RR42_RS33730 0.41 -0.1 2,994,777 + RR42_RS33730 0.41 +1.0 2,994,777 + RR42_RS33730 0.41 -0.2 2,994,778 - RR42_RS33730 0.41 -1.0 2,994,778 - RR42_RS33730 0.41 -0.8 2,994,984 - RR42_RS33730 0.57 -1.0 2,995,147 - RR42_RS33730 0.70 -0.6 2,995,437 + -0.3 2,995,438 - -0.8 2,995,502 + -0.8 2,995,503 - -0.7 2,995,717 + -0.2 2,995,718 - -0.0 2,995,882 + RR42_RS33735 0.21 +2.1 2,995,899 + RR42_RS33735 0.22 -0.1 2,995,997 - RR42_RS33735 0.29 -0.4 2,995,997 - RR42_RS33735 0.29 -0.6 2,996,110 + RR42_RS33735 0.37 +0.3 2,996,110 + RR42_RS33735 0.37 +1.2 2,996,111 - RR42_RS33735 0.37 +0.4 2,996,468 - RR42_RS33735 0.62 -0.1 2,997,016 + +0.7 2,997,075 - +0.1 2,997,478 - RR42_RS33740 0.36 +0.8 2,997,478 - RR42_RS33740 0.36 -0.5 2,997,478 - RR42_RS33740 0.36 +0.3 2,997,481 + RR42_RS33740 0.36 -0.4 2,997,481 + RR42_RS33740 0.36 +0.6 2,997,482 - RR42_RS33740 0.36 -0.3 2,997,741 + RR42_RS33740 0.56 +0.3 2,997,762 - RR42_RS33740 0.58 +1.5 2,997,806 + RR42_RS33740 0.61 +0.4 2,997,806 + RR42_RS33740 0.61 -1.0 2,997,807 - RR42_RS33740 0.61 +0.1 2,997,924 - RR42_RS33740 0.70 -0.4 2,997,968 - RR42_RS33740 0.74 -0.3
Or see this region's nucleotide sequence