Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS33585

Experiment: R2A with Sisomicin 0.035 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS33580 and RR42_RS33585 are separated by 290 nucleotidesRR42_RS33585 and RR42_RS33590 are separated by 104 nucleotidesRR42_RS33590 and RR42_RS33595 are separated by 11 nucleotides RR42_RS33580: RR42_RS33580 - 3-hydroxyacyl-CoA dehydrogenase, at 2,962,136 to 2,963,050 _RS33580 RR42_RS33585: RR42_RS33585 - 3-keto-5-aminohexanoate cleavage protein, at 2,963,341 to 2,964,273 _RS33585 RR42_RS33590: RR42_RS33590 - cyclase, at 2,964,378 to 2,965,136 _RS33590 RR42_RS33595: RR42_RS33595 - hypothetical protein, at 2,965,148 to 2,965,522 _RS33595 Position (kb) 2963 2964 2965Strain fitness (log2 ratio) -3 -2 -1 0 1at 2962.541 kb on + strand, within RR42_RS33580at 2962.715 kb on + strand, within RR42_RS33580at 2962.715 kb on + strand, within RR42_RS33580at 2962.755 kb on - strand, within RR42_RS33580at 2962.839 kb on - strand, within RR42_RS33580at 2962.997 kb on + strandat 2962.997 kb on + strandat 2963.044 kb on + strandat 2963.044 kb on + strandat 2963.044 kb on + strandat 2963.044 kb on + strandat 2963.044 kb on + strandat 2963.044 kb on + strandat 2963.044 kb on + strandat 2963.045 kb on - strandat 2963.045 kb on - strandat 2963.045 kb on - strandat 2963.045 kb on - strandat 2963.045 kb on - strandat 2963.045 kb on - strandat 2963.045 kb on - strandat 2963.411 kb on - strandat 2963.538 kb on + strand, within RR42_RS33585at 2963.538 kb on + strand, within RR42_RS33585at 2963.749 kb on + strand, within RR42_RS33585at 2963.749 kb on + strand, within RR42_RS33585at 2963.749 kb on + strand, within RR42_RS33585at 2963.749 kb on + strand, within RR42_RS33585at 2963.750 kb on - strand, within RR42_RS33585at 2963.769 kb on + strand, within RR42_RS33585at 2963.769 kb on + strand, within RR42_RS33585at 2963.770 kb on - strand, within RR42_RS33585at 2963.770 kb on - strand, within RR42_RS33585at 2963.784 kb on + strand, within RR42_RS33585at 2963.785 kb on - strand, within RR42_RS33585at 2963.785 kb on - strand, within RR42_RS33585at 2963.857 kb on + strand, within RR42_RS33585at 2963.857 kb on + strand, within RR42_RS33585at 2963.858 kb on - strand, within RR42_RS33585at 2963.858 kb on - strand, within RR42_RS33585at 2963.858 kb on - strand, within RR42_RS33585at 2963.864 kb on + strand, within RR42_RS33585at 2963.864 kb on + strand, within RR42_RS33585at 2963.864 kb on + strand, within RR42_RS33585at 2963.864 kb on + strand, within RR42_RS33585at 2963.875 kb on + strand, within RR42_RS33585at 2964.000 kb on + strand, within RR42_RS33585at 2964.022 kb on + strand, within RR42_RS33585at 2964.023 kb on - strand, within RR42_RS33585at 2964.023 kb on - strand, within RR42_RS33585at 2964.025 kb on + strand, within RR42_RS33585at 2964.025 kb on + strand, within RR42_RS33585at 2964.026 kb on - strand, within RR42_RS33585at 2964.074 kb on - strand, within RR42_RS33585at 2964.123 kb on + strand, within RR42_RS33585at 2964.124 kb on + strand, within RR42_RS33585at 2964.125 kb on - strand, within RR42_RS33585at 2964.125 kb on - strand, within RR42_RS33585at 2964.286 kb on + strandat 2964.286 kb on + strandat 2964.331 kb on - strandat 2964.407 kb on + strandat 2964.485 kb on + strand, within RR42_RS33590at 2964.506 kb on + strand, within RR42_RS33590at 2964.655 kb on - strand, within RR42_RS33590at 2964.730 kb on - strand, within RR42_RS33590at 2964.733 kb on - strand, within RR42_RS33590at 2964.739 kb on - strand, within RR42_RS33590at 2964.753 kb on + strand, within RR42_RS33590at 2964.753 kb on + strand, within RR42_RS33590at 2964.754 kb on - strand, within RR42_RS33590at 2964.754 kb on - strand, within RR42_RS33590at 2964.892 kb on - strand, within RR42_RS33590at 2964.892 kb on - strand, within RR42_RS33590at 2965.078 kb on + strandat 2965.078 kb on + strandat 2965.078 kb on + strandat 2965.079 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml
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2,962,541 + RR42_RS33580 0.44 +0.4
2,962,715 + RR42_RS33580 0.63 +0.8
2,962,715 + RR42_RS33580 0.63 +1.3
2,962,755 - RR42_RS33580 0.68 -0.7
2,962,839 - RR42_RS33580 0.77 +1.0
2,962,997 + -0.6
2,962,997 + -0.1
2,963,044 + +0.3
2,963,044 + -1.6
2,963,044 + -1.7
2,963,044 + -1.0
2,963,044 + -3.6
2,963,044 + -1.8
2,963,044 + +0.5
2,963,045 - -0.1
2,963,045 - -1.3
2,963,045 - -1.0
2,963,045 - -1.0
2,963,045 - -0.3
2,963,045 - -1.1
2,963,045 - +0.1
2,963,411 - +0.0
2,963,538 + RR42_RS33585 0.21 +0.1
2,963,538 + RR42_RS33585 0.21 -0.3
2,963,749 + RR42_RS33585 0.44 +1.3
2,963,749 + RR42_RS33585 0.44 +0.3
2,963,749 + RR42_RS33585 0.44 -0.9
2,963,749 + RR42_RS33585 0.44 -1.1
2,963,750 - RR42_RS33585 0.44 -0.2
2,963,769 + RR42_RS33585 0.46 -0.3
2,963,769 + RR42_RS33585 0.46 -0.6
2,963,770 - RR42_RS33585 0.46 +0.4
2,963,770 - RR42_RS33585 0.46 +0.4
2,963,784 + RR42_RS33585 0.47 +0.6
2,963,785 - RR42_RS33585 0.48 -0.3
2,963,785 - RR42_RS33585 0.48 +0.4
2,963,857 + RR42_RS33585 0.55 +0.2
2,963,857 + RR42_RS33585 0.55 +0.6
2,963,858 - RR42_RS33585 0.55 +0.6
2,963,858 - RR42_RS33585 0.55 -0.8
2,963,858 - RR42_RS33585 0.55 -0.2
2,963,864 + RR42_RS33585 0.56 -0.1
2,963,864 + RR42_RS33585 0.56 -0.4
2,963,864 + RR42_RS33585 0.56 -0.5
2,963,864 + RR42_RS33585 0.56 +1.2
2,963,875 + RR42_RS33585 0.57 +1.2
2,964,000 + RR42_RS33585 0.71 +0.5
2,964,022 + RR42_RS33585 0.73 +0.3
2,964,023 - RR42_RS33585 0.73 +0.0
2,964,023 - RR42_RS33585 0.73 -0.9
2,964,025 + RR42_RS33585 0.73 -0.5
2,964,025 + RR42_RS33585 0.73 +0.1
2,964,026 - RR42_RS33585 0.73 -0.1
2,964,074 - RR42_RS33585 0.79 +0.3
2,964,123 + RR42_RS33585 0.84 +0.2
2,964,124 + RR42_RS33585 0.84 +1.6
2,964,125 - RR42_RS33585 0.84 -0.0
2,964,125 - RR42_RS33585 0.84 +1.0
2,964,286 + -0.2
2,964,286 + +1.5
2,964,331 - +0.5
2,964,407 + +0.1
2,964,485 + RR42_RS33590 0.14 -0.6
2,964,506 + RR42_RS33590 0.17 +0.1
2,964,655 - RR42_RS33590 0.36 +0.3
2,964,730 - RR42_RS33590 0.46 -0.8
2,964,733 - RR42_RS33590 0.47 +1.5
2,964,739 - RR42_RS33590 0.48 +0.7
2,964,753 + RR42_RS33590 0.49 +0.2
2,964,753 + RR42_RS33590 0.49 -1.1
2,964,754 - RR42_RS33590 0.50 +0.4
2,964,754 - RR42_RS33590 0.50 -1.0
2,964,892 - RR42_RS33590 0.68 +0.6
2,964,892 - RR42_RS33590 0.68 +0.2
2,965,078 + +0.4
2,965,078 + +0.2
2,965,078 + -1.2
2,965,079 - -0.3

Or see this region's nucleotide sequence