Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33415 and RR42_RS33420 are separated by 2 nucleotides RR42_RS33420 and RR42_RS33425 are separated by 193 nucleotides
RR42_RS33415: RR42_RS33415 - RND transporter MFP subunit, at 2,914,919 to 2,916,079
_RS33415
RR42_RS33420: RR42_RS33420 - membrane protein, at 2,916,082 to 2,917,353
_RS33420
RR42_RS33425: RR42_RS33425 - cobalt-zinc-cadmium resistance protein, at 2,917,547 to 2,918,026
_RS33425
Position (kb)
2916
2917
2918 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2915.082 kb on + strand, within RR42_RS33415 at 2915.262 kb on + strand, within RR42_RS33415 at 2915.262 kb on + strand, within RR42_RS33415 at 2915.262 kb on + strand, within RR42_RS33415 at 2915.262 kb on + strand, within RR42_RS33415 at 2915.262 kb on + strand, within RR42_RS33415 at 2915.263 kb on - strand, within RR42_RS33415 at 2915.412 kb on + strand, within RR42_RS33415 at 2915.499 kb on + strand, within RR42_RS33415 at 2915.753 kb on - strand, within RR42_RS33415 at 2915.753 kb on - strand, within RR42_RS33415 at 2915.753 kb on - strand, within RR42_RS33415 at 2916.058 kb on + strand at 2916.058 kb on + strand at 2916.059 kb on - strand at 2916.062 kb on + strand at 2916.063 kb on - strand at 2916.083 kb on + strand at 2916.083 kb on + strand at 2916.083 kb on + strand at 2916.084 kb on - strand at 2916.096 kb on + strand at 2916.096 kb on + strand at 2916.251 kb on + strand, within RR42_RS33420 at 2916.251 kb on + strand, within RR42_RS33420 at 2916.281 kb on + strand, within RR42_RS33420 at 2916.346 kb on + strand, within RR42_RS33420 at 2916.346 kb on + strand, within RR42_RS33420 at 2916.590 kb on + strand, within RR42_RS33420 at 2916.591 kb on - strand, within RR42_RS33420 at 2916.726 kb on - strand, within RR42_RS33420 at 2916.726 kb on - strand, within RR42_RS33420 at 2916.726 kb on - strand, within RR42_RS33420 at 2916.851 kb on + strand, within RR42_RS33420 at 2916.955 kb on + strand, within RR42_RS33420 at 2916.955 kb on + strand, within RR42_RS33420 at 2916.956 kb on - strand, within RR42_RS33420 at 2916.968 kb on + strand, within RR42_RS33420 at 2916.969 kb on - strand, within RR42_RS33420 at 2917.080 kb on - strand, within RR42_RS33420 at 2917.157 kb on + strand, within RR42_RS33420 at 2917.158 kb on - strand, within RR42_RS33420 at 2917.158 kb on - strand, within RR42_RS33420 at 2917.158 kb on - strand, within RR42_RS33420 at 2917.497 kb on + strand at 2917.498 kb on - strand at 2917.943 kb on + strand, within RR42_RS33425 at 2917.945 kb on + strand, within RR42_RS33425 at 2917.945 kb on + strand, within RR42_RS33425 at 2917.945 kb on + strand, within RR42_RS33425 at 2917.946 kb on - strand, within RR42_RS33425 at 2918.037 kb on - strand at 2918.101 kb on + strand at 2918.101 kb on + strand at 2918.153 kb on - strand at 2918.153 kb on - strand at 2918.153 kb on - strand at 2918.159 kb on + strand at 2918.159 kb on + strand at 2918.160 kb on - strand at 2918.160 kb on - strand at 2918.160 kb on - strand at 2918.324 kb on + strand at 2918.325 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,915,082 + RR42_RS33415 0.14 -0.1 2,915,262 + RR42_RS33415 0.30 -1.3 2,915,262 + RR42_RS33415 0.30 -0.7 2,915,262 + RR42_RS33415 0.30 -0.3 2,915,262 + RR42_RS33415 0.30 -0.2 2,915,262 + RR42_RS33415 0.30 -0.6 2,915,263 - RR42_RS33415 0.30 +1.0 2,915,412 + RR42_RS33415 0.42 +0.1 2,915,499 + RR42_RS33415 0.50 +0.8 2,915,753 - RR42_RS33415 0.72 +0.4 2,915,753 - RR42_RS33415 0.72 -0.1 2,915,753 - RR42_RS33415 0.72 +0.2 2,916,058 + -0.1 2,916,058 + +2.0 2,916,059 - +1.0 2,916,062 + -0.4 2,916,063 - +0.0 2,916,083 + -1.4 2,916,083 + -0.3 2,916,083 + +1.4 2,916,084 - -1.1 2,916,096 + +1.0 2,916,096 + -0.4 2,916,251 + RR42_RS33420 0.13 +0.0 2,916,251 + RR42_RS33420 0.13 +0.2 2,916,281 + RR42_RS33420 0.16 -1.8 2,916,346 + RR42_RS33420 0.21 +0.4 2,916,346 + RR42_RS33420 0.21 +0.5 2,916,590 + RR42_RS33420 0.40 -0.4 2,916,591 - RR42_RS33420 0.40 -1.1 2,916,726 - RR42_RS33420 0.51 -0.5 2,916,726 - RR42_RS33420 0.51 -0.8 2,916,726 - RR42_RS33420 0.51 -0.1 2,916,851 + RR42_RS33420 0.60 -1.2 2,916,955 + RR42_RS33420 0.69 -0.6 2,916,955 + RR42_RS33420 0.69 -1.3 2,916,956 - RR42_RS33420 0.69 -0.2 2,916,968 + RR42_RS33420 0.70 -0.5 2,916,969 - RR42_RS33420 0.70 -2.4 2,917,080 - RR42_RS33420 0.78 -1.0 2,917,157 + RR42_RS33420 0.85 -0.1 2,917,158 - RR42_RS33420 0.85 -0.9 2,917,158 - RR42_RS33420 0.85 -0.9 2,917,158 - RR42_RS33420 0.85 -1.6 2,917,497 + +0.3 2,917,498 - +0.0 2,917,943 + RR42_RS33425 0.82 -0.7 2,917,945 + RR42_RS33425 0.83 -0.8 2,917,945 + RR42_RS33425 0.83 -0.2 2,917,945 + RR42_RS33425 0.83 +1.0 2,917,946 - RR42_RS33425 0.83 -0.9 2,918,037 - -0.9 2,918,101 + +0.2 2,918,101 + -0.5 2,918,153 - -2.3 2,918,153 - -1.0 2,918,153 - -1.1 2,918,159 + +0.2 2,918,159 + -0.7 2,918,160 - -0.6 2,918,160 - -1.0 2,918,160 - +0.1 2,918,324 + -0.2 2,918,325 - -0.7
Or see this region's nucleotide sequence