Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33235 and RR42_RS33240 are separated by 29 nucleotides RR42_RS33240 and RR42_RS33245 are separated by 8 nucleotides
RR42_RS33235: RR42_RS33235 - GDP-mannose 4,6-dehydratase, at 2,870,466 to 2,871,554
_RS33235
RR42_RS33240: RR42_RS33240 - GDP-L-fucose synthase, at 2,871,584 to 2,872,516
_RS33240
RR42_RS33245: RR42_RS33245 - mannose-1-phosphate guanyltransferase, at 2,872,525 to 2,873,949
_RS33245
Position (kb)
2870
2871
2872 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2870.780 kb on - strand, within RR42_RS33235 at 2870.780 kb on - strand, within RR42_RS33235 at 2870.844 kb on - strand, within RR42_RS33235 at 2870.993 kb on + strand, within RR42_RS33235 at 2870.993 kb on + strand, within RR42_RS33235 at 2870.994 kb on - strand, within RR42_RS33235 at 2870.994 kb on - strand, within RR42_RS33235 at 2870.997 kb on + strand, within RR42_RS33235 at 2870.998 kb on - strand, within RR42_RS33235 at 2871.017 kb on - strand, within RR42_RS33235 at 2871.061 kb on + strand, within RR42_RS33235 at 2871.061 kb on + strand, within RR42_RS33235 at 2871.061 kb on + strand, within RR42_RS33235 at 2871.062 kb on - strand, within RR42_RS33235 at 2871.069 kb on - strand, within RR42_RS33235 at 2871.069 kb on - strand, within RR42_RS33235 at 2871.163 kb on + strand, within RR42_RS33235 at 2871.212 kb on + strand, within RR42_RS33235 at 2871.213 kb on - strand, within RR42_RS33235 at 2871.246 kb on - strand, within RR42_RS33235 at 2871.246 kb on - strand, within RR42_RS33235 at 2871.299 kb on - strand, within RR42_RS33235 at 2871.407 kb on - strand, within RR42_RS33235 at 2871.410 kb on + strand, within RR42_RS33235 at 2871.410 kb on + strand, within RR42_RS33235 at 2871.431 kb on + strand, within RR42_RS33235 at 2871.500 kb on - strand at 2871.563 kb on - strand at 2871.582 kb on - strand at 2871.585 kb on + strand at 2871.586 kb on - strand at 2871.681 kb on - strand, within RR42_RS33240 at 2871.726 kb on + strand, within RR42_RS33240 at 2871.726 kb on + strand, within RR42_RS33240 at 2871.727 kb on - strand, within RR42_RS33240 at 2871.728 kb on + strand, within RR42_RS33240 at 2871.788 kb on + strand, within RR42_RS33240 at 2871.789 kb on - strand, within RR42_RS33240 at 2871.803 kb on - strand, within RR42_RS33240 at 2871.807 kb on - strand, within RR42_RS33240 at 2871.808 kb on + strand, within RR42_RS33240 at 2871.809 kb on - strand, within RR42_RS33240 at 2871.812 kb on + strand, within RR42_RS33240 at 2871.813 kb on - strand, within RR42_RS33240 at 2871.828 kb on + strand, within RR42_RS33240 at 2871.829 kb on - strand, within RR42_RS33240 at 2871.848 kb on + strand, within RR42_RS33240 at 2871.848 kb on + strand, within RR42_RS33240 at 2871.849 kb on - strand, within RR42_RS33240 at 2871.849 kb on - strand, within RR42_RS33240 at 2871.872 kb on + strand, within RR42_RS33240 at 2871.872 kb on + strand, within RR42_RS33240 at 2871.873 kb on - strand, within RR42_RS33240 at 2871.912 kb on - strand, within RR42_RS33240 at 2871.918 kb on + strand, within RR42_RS33240 at 2871.936 kb on - strand, within RR42_RS33240 at 2871.936 kb on - strand, within RR42_RS33240 at 2871.972 kb on - strand, within RR42_RS33240 at 2871.982 kb on + strand, within RR42_RS33240 at 2871.982 kb on + strand, within RR42_RS33240 at 2871.983 kb on - strand, within RR42_RS33240 at 2871.983 kb on - strand, within RR42_RS33240 at 2871.990 kb on - strand, within RR42_RS33240 at 2871.990 kb on - strand, within RR42_RS33240 at 2871.997 kb on - strand, within RR42_RS33240 at 2871.997 kb on - strand, within RR42_RS33240 at 2871.997 kb on - strand, within RR42_RS33240 at 2871.997 kb on - strand, within RR42_RS33240 at 2872.037 kb on + strand, within RR42_RS33240 at 2872.038 kb on - strand, within RR42_RS33240 at 2872.038 kb on - strand, within RR42_RS33240 at 2872.041 kb on + strand, within RR42_RS33240 at 2872.041 kb on + strand, within RR42_RS33240 at 2872.042 kb on - strand, within RR42_RS33240 at 2872.042 kb on - strand, within RR42_RS33240 at 2872.065 kb on + strand, within RR42_RS33240 at 2872.065 kb on + strand, within RR42_RS33240 at 2872.066 kb on - strand, within RR42_RS33240 at 2872.108 kb on - strand, within RR42_RS33240 at 2872.146 kb on + strand, within RR42_RS33240 at 2872.146 kb on + strand, within RR42_RS33240 at 2872.147 kb on - strand, within RR42_RS33240 at 2872.147 kb on - strand, within RR42_RS33240 at 2872.182 kb on + strand, within RR42_RS33240 at 2872.182 kb on + strand, within RR42_RS33240 at 2872.186 kb on + strand, within RR42_RS33240 at 2872.186 kb on + strand, within RR42_RS33240 at 2872.186 kb on + strand, within RR42_RS33240 at 2872.186 kb on + strand, within RR42_RS33240 at 2872.187 kb on - strand, within RR42_RS33240 at 2872.187 kb on - strand, within RR42_RS33240 at 2872.187 kb on - strand, within RR42_RS33240 at 2872.290 kb on + strand, within RR42_RS33240 at 2872.291 kb on - strand, within RR42_RS33240 at 2872.292 kb on + strand, within RR42_RS33240 at 2872.292 kb on + strand, within RR42_RS33240 at 2872.293 kb on - strand, within RR42_RS33240 at 2872.293 kb on - strand, within RR42_RS33240 at 2872.342 kb on - strand, within RR42_RS33240 at 2872.397 kb on + strand, within RR42_RS33240 at 2872.397 kb on + strand, within RR42_RS33240 at 2872.398 kb on - strand, within RR42_RS33240 at 2872.398 kb on - strand, within RR42_RS33240 at 2872.431 kb on + strand at 2872.459 kb on + strand at 2872.459 kb on + strand at 2872.459 kb on + strand at 2872.460 kb on - strand at 2872.510 kb on + strand at 2872.510 kb on + strand at 2872.511 kb on - strand at 2872.538 kb on + strand at 2872.539 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,870,780 - RR42_RS33235 0.29 -0.2 2,870,780 - RR42_RS33235 0.29 -2.0 2,870,844 - RR42_RS33235 0.35 -2.7 2,870,993 + RR42_RS33235 0.48 -1.9 2,870,993 + RR42_RS33235 0.48 -1.0 2,870,994 - RR42_RS33235 0.48 -1.5 2,870,994 - RR42_RS33235 0.48 -0.1 2,870,997 + RR42_RS33235 0.49 -2.0 2,870,998 - RR42_RS33235 0.49 -2.3 2,871,017 - RR42_RS33235 0.51 -2.8 2,871,061 + RR42_RS33235 0.55 -3.9 2,871,061 + RR42_RS33235 0.55 -1.3 2,871,061 + RR42_RS33235 0.55 +1.0 2,871,062 - RR42_RS33235 0.55 +0.0 2,871,069 - RR42_RS33235 0.55 -2.2 2,871,069 - RR42_RS33235 0.55 -1.8 2,871,163 + RR42_RS33235 0.64 -1.1 2,871,212 + RR42_RS33235 0.69 -2.2 2,871,213 - RR42_RS33235 0.69 -2.3 2,871,246 - RR42_RS33235 0.72 -0.8 2,871,246 - RR42_RS33235 0.72 -3.2 2,871,299 - RR42_RS33235 0.76 -2.4 2,871,407 - RR42_RS33235 0.86 -1.0 2,871,410 + RR42_RS33235 0.87 -1.3 2,871,410 + RR42_RS33235 0.87 +1.5 2,871,431 + RR42_RS33235 0.89 -1.5 2,871,500 - -2.5 2,871,563 - -0.4 2,871,582 - -1.2 2,871,585 + -1.6 2,871,586 - -1.2 2,871,681 - RR42_RS33240 0.10 -0.6 2,871,726 + RR42_RS33240 0.15 -1.4 2,871,726 + RR42_RS33240 0.15 -2.3 2,871,727 - RR42_RS33240 0.15 -1.3 2,871,728 + RR42_RS33240 0.15 -2.2 2,871,788 + RR42_RS33240 0.22 -0.9 2,871,789 - RR42_RS33240 0.22 -1.5 2,871,803 - RR42_RS33240 0.23 -2.5 2,871,807 - RR42_RS33240 0.24 -1.0 2,871,808 + RR42_RS33240 0.24 -2.0 2,871,809 - RR42_RS33240 0.24 -0.6 2,871,812 + RR42_RS33240 0.24 -1.7 2,871,813 - RR42_RS33240 0.25 -0.6 2,871,828 + RR42_RS33240 0.26 -1.3 2,871,829 - RR42_RS33240 0.26 -1.5 2,871,848 + RR42_RS33240 0.28 -1.9 2,871,848 + RR42_RS33240 0.28 -0.3 2,871,849 - RR42_RS33240 0.28 -0.8 2,871,849 - RR42_RS33240 0.28 -1.1 2,871,872 + RR42_RS33240 0.31 -0.6 2,871,872 + RR42_RS33240 0.31 -1.6 2,871,873 - RR42_RS33240 0.31 -3.2 2,871,912 - RR42_RS33240 0.35 -1.6 2,871,918 + RR42_RS33240 0.36 +0.0 2,871,936 - RR42_RS33240 0.38 -2.2 2,871,936 - RR42_RS33240 0.38 -2.1 2,871,972 - RR42_RS33240 0.42 -2.7 2,871,982 + RR42_RS33240 0.43 -1.2 2,871,982 + RR42_RS33240 0.43 -2.3 2,871,983 - RR42_RS33240 0.43 -1.0 2,871,983 - RR42_RS33240 0.43 +0.3 2,871,990 - RR42_RS33240 0.44 -0.6 2,871,990 - RR42_RS33240 0.44 -1.0 2,871,997 - RR42_RS33240 0.44 -1.6 2,871,997 - RR42_RS33240 0.44 -1.9 2,871,997 - RR42_RS33240 0.44 -2.0 2,871,997 - RR42_RS33240 0.44 -1.8 2,872,037 + RR42_RS33240 0.49 -3.8 2,872,038 - RR42_RS33240 0.49 -0.8 2,872,038 - RR42_RS33240 0.49 +0.3 2,872,041 + RR42_RS33240 0.49 -2.3 2,872,041 + RR42_RS33240 0.49 -1.0 2,872,042 - RR42_RS33240 0.49 +1.0 2,872,042 - RR42_RS33240 0.49 -0.8 2,872,065 + RR42_RS33240 0.52 -1.7 2,872,065 + RR42_RS33240 0.52 -0.2 2,872,066 - RR42_RS33240 0.52 +0.1 2,872,108 - RR42_RS33240 0.56 -2.7 2,872,146 + RR42_RS33240 0.60 -3.5 2,872,146 + RR42_RS33240 0.60 -1.0 2,872,147 - RR42_RS33240 0.60 -1.0 2,872,147 - RR42_RS33240 0.60 -1.3 2,872,182 + RR42_RS33240 0.64 -4.0 2,872,182 + RR42_RS33240 0.64 -2.9 2,872,186 + RR42_RS33240 0.65 -2.2 2,872,186 + RR42_RS33240 0.65 +0.0 2,872,186 + RR42_RS33240 0.65 -3.9 2,872,186 + RR42_RS33240 0.65 -0.6 2,872,187 - RR42_RS33240 0.65 -2.2 2,872,187 - RR42_RS33240 0.65 -1.0 2,872,187 - RR42_RS33240 0.65 -3.3 2,872,290 + RR42_RS33240 0.76 -1.3 2,872,291 - RR42_RS33240 0.76 -0.2 2,872,292 + RR42_RS33240 0.76 -4.6 2,872,292 + RR42_RS33240 0.76 -2.2 2,872,293 - RR42_RS33240 0.76 -0.5 2,872,293 - RR42_RS33240 0.76 -1.2 2,872,342 - RR42_RS33240 0.81 -1.8 2,872,397 + RR42_RS33240 0.87 -0.3 2,872,397 + RR42_RS33240 0.87 -4.4 2,872,398 - RR42_RS33240 0.87 -1.8 2,872,398 - RR42_RS33240 0.87 -1.6 2,872,431 + -2.5 2,872,459 + +1.0 2,872,459 + -3.0 2,872,459 + +0.0 2,872,460 - -3.3 2,872,510 + -0.6 2,872,510 + -0.6 2,872,511 - -2.0 2,872,538 + -0.8 2,872,539 - +0.0
Or see this region's nucleotide sequence