Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33085 and RR42_RS33090 are separated by 99 nucleotides RR42_RS33090 and RR42_RS33095 are separated by 326 nucleotides
RR42_RS33085: RR42_RS33085 - ligand-gated channel protein, at 2,828,698 to 2,830,917
_RS33085
RR42_RS33090: RR42_RS33090 - fumarate hydratase, at 2,831,017 to 2,832,420
_RS33090
RR42_RS33095: RR42_RS33095 - LysR family transcriptional regulator, at 2,832,747 to 2,833,652
_RS33095
Position (kb)
2831
2832
2833 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2830.060 kb on + strand, within RR42_RS33085 at 2830.189 kb on + strand, within RR42_RS33085 at 2830.189 kb on + strand, within RR42_RS33085 at 2830.234 kb on + strand, within RR42_RS33085 at 2830.234 kb on + strand, within RR42_RS33085 at 2830.234 kb on + strand, within RR42_RS33085 at 2830.234 kb on + strand, within RR42_RS33085 at 2830.234 kb on + strand, within RR42_RS33085 at 2830.235 kb on - strand, within RR42_RS33085 at 2830.543 kb on + strand, within RR42_RS33085 at 2830.649 kb on - strand, within RR42_RS33085 at 2830.649 kb on - strand, within RR42_RS33085 at 2830.651 kb on + strand, within RR42_RS33085 at 2830.651 kb on + strand, within RR42_RS33085 at 2830.652 kb on - strand, within RR42_RS33085 at 2830.652 kb on - strand, within RR42_RS33085 at 2830.652 kb on - strand, within RR42_RS33085 at 2830.708 kb on + strand at 2830.708 kb on + strand at 2830.708 kb on + strand at 2830.709 kb on - strand at 2830.778 kb on - strand at 2830.837 kb on + strand at 2830.837 kb on + strand at 2830.837 kb on + strand at 2830.837 kb on + strand at 2830.838 kb on - strand at 2830.838 kb on - strand at 2830.840 kb on + strand at 2830.862 kb on - strand at 2830.911 kb on + strand at 2830.911 kb on + strand at 2830.911 kb on + strand at 2830.911 kb on + strand at 2830.912 kb on - strand at 2830.912 kb on - strand at 2830.912 kb on - strand at 2830.912 kb on - strand at 2830.915 kb on + strand at 2830.915 kb on + strand at 2830.915 kb on + strand at 2830.915 kb on + strand at 2830.915 kb on + strand at 2830.915 kb on + strand at 2830.915 kb on + strand at 2830.915 kb on + strand at 2830.915 kb on + strand at 2830.916 kb on - strand at 2830.916 kb on - strand at 2830.951 kb on + strand at 2830.951 kb on + strand at 2831.067 kb on - strand at 2831.079 kb on + strand at 2831.080 kb on - strand at 2831.084 kb on + strand at 2831.084 kb on + strand at 2831.085 kb on - strand at 2831.085 kb on - strand at 2831.170 kb on - strand, within RR42_RS33090 at 2831.334 kb on - strand, within RR42_RS33090 at 2831.667 kb on + strand, within RR42_RS33090 at 2831.669 kb on + strand, within RR42_RS33090 at 2831.669 kb on + strand, within RR42_RS33090 at 2831.669 kb on + strand, within RR42_RS33090 at 2831.669 kb on + strand, within RR42_RS33090 at 2831.670 kb on - strand, within RR42_RS33090 at 2831.670 kb on - strand, within RR42_RS33090 at 2831.944 kb on - strand, within RR42_RS33090 at 2832.327 kb on + strand at 2832.443 kb on + strand at 2832.443 kb on + strand at 2832.443 kb on + strand at 2832.443 kb on + strand at 2832.444 kb on - strand at 2832.700 kb on + strand at 2832.701 kb on - strand at 2832.701 kb on - strand at 2832.704 kb on + strand at 2832.704 kb on + strand at 2832.705 kb on - strand at 2832.708 kb on - strand at 2832.717 kb on + strand at 2832.748 kb on + strand at 2832.749 kb on - strand at 2832.772 kb on + strand at 2832.772 kb on + strand at 2832.773 kb on - strand at 2832.773 kb on - strand at 2832.823 kb on + strand at 2832.824 kb on - strand at 2832.824 kb on - strand at 2832.920 kb on - strand, within RR42_RS33095 at 2832.920 kb on - strand, within RR42_RS33095 at 2832.920 kb on - strand, within RR42_RS33095 at 2832.920 kb on - strand, within RR42_RS33095 at 2832.954 kb on + strand, within RR42_RS33095 at 2832.955 kb on - strand, within RR42_RS33095 at 2832.955 kb on - strand, within RR42_RS33095 at 2832.955 kb on - strand, within RR42_RS33095 at 2833.132 kb on + strand, within RR42_RS33095 at 2833.132 kb on + strand, within RR42_RS33095 at 2833.132 kb on + strand, within RR42_RS33095 at 2833.287 kb on + strand, within RR42_RS33095 at 2833.287 kb on + strand, within RR42_RS33095 at 2833.288 kb on - strand, within RR42_RS33095 at 2833.288 kb on - strand, within RR42_RS33095 at 2833.288 kb on - strand, within RR42_RS33095 at 2833.288 kb on - strand, within RR42_RS33095 at 2833.288 kb on - strand, within RR42_RS33095 at 2833.356 kb on + strand, within RR42_RS33095 at 2833.356 kb on + strand, within RR42_RS33095 at 2833.356 kb on + strand, within RR42_RS33095 at 2833.357 kb on - strand, within RR42_RS33095 at 2833.357 kb on - strand, within RR42_RS33095 at 2833.357 kb on - strand, within RR42_RS33095 at 2833.357 kb on - strand, within RR42_RS33095
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,830,060 + RR42_RS33085 0.61 +0.1 2,830,189 + RR42_RS33085 0.67 -0.1 2,830,189 + RR42_RS33085 0.67 +0.4 2,830,234 + RR42_RS33085 0.69 -0.2 2,830,234 + RR42_RS33085 0.69 -0.4 2,830,234 + RR42_RS33085 0.69 +0.0 2,830,234 + RR42_RS33085 0.69 +0.8 2,830,234 + RR42_RS33085 0.69 +1.2 2,830,235 - RR42_RS33085 0.69 -1.0 2,830,543 + RR42_RS33085 0.83 -0.2 2,830,649 - RR42_RS33085 0.88 -1.7 2,830,649 - RR42_RS33085 0.88 -0.1 2,830,651 + RR42_RS33085 0.88 +0.4 2,830,651 + RR42_RS33085 0.88 -0.4 2,830,652 - RR42_RS33085 0.88 +0.1 2,830,652 - RR42_RS33085 0.88 -0.5 2,830,652 - RR42_RS33085 0.88 +0.0 2,830,708 + -2.8 2,830,708 + +0.1 2,830,708 + +1.8 2,830,709 - +0.3 2,830,778 - +0.3 2,830,837 + -1.3 2,830,837 + +0.2 2,830,837 + +0.0 2,830,837 + -1.2 2,830,838 - -0.6 2,830,838 - -0.4 2,830,840 + -0.2 2,830,862 - +0.0 2,830,911 + +0.5 2,830,911 + +0.0 2,830,911 + -0.5 2,830,911 + -0.1 2,830,912 - -0.6 2,830,912 - -2.4 2,830,912 - +0.4 2,830,912 - +0.0 2,830,915 + -0.2 2,830,915 + +0.6 2,830,915 + +0.2 2,830,915 + -0.2 2,830,915 + +0.6 2,830,915 + +1.4 2,830,915 + -0.7 2,830,915 + +0.7 2,830,915 + +2.3 2,830,916 - +0.5 2,830,916 - +0.9 2,830,951 + +0.0 2,830,951 + +0.2 2,831,067 - -0.4 2,831,079 + +0.3 2,831,080 - +0.4 2,831,084 + +0.6 2,831,084 + +0.0 2,831,085 - +0.9 2,831,085 - +0.0 2,831,170 - RR42_RS33090 0.11 +0.3 2,831,334 - RR42_RS33090 0.23 -0.1 2,831,667 + RR42_RS33090 0.46 -0.7 2,831,669 + RR42_RS33090 0.46 +0.3 2,831,669 + RR42_RS33090 0.46 -1.3 2,831,669 + RR42_RS33090 0.46 -0.4 2,831,669 + RR42_RS33090 0.46 -1.3 2,831,670 - RR42_RS33090 0.47 +0.1 2,831,670 - RR42_RS33090 0.47 -0.1 2,831,944 - RR42_RS33090 0.66 -1.3 2,832,327 + -0.2 2,832,443 + -0.5 2,832,443 + -0.5 2,832,443 + +0.8 2,832,443 + -0.2 2,832,444 - +0.7 2,832,700 + +0.3 2,832,701 - +0.4 2,832,701 - +0.0 2,832,704 + +0.6 2,832,704 + +0.1 2,832,705 - -0.3 2,832,708 - +0.4 2,832,717 + -0.5 2,832,748 + +0.5 2,832,749 - +0.7 2,832,772 + +1.4 2,832,772 + -1.8 2,832,773 - -1.0 2,832,773 - -0.0 2,832,823 + +0.3 2,832,824 - +0.4 2,832,824 - -1.1 2,832,920 - RR42_RS33095 0.19 +0.3 2,832,920 - RR42_RS33095 0.19 +0.6 2,832,920 - RR42_RS33095 0.19 -0.7 2,832,920 - RR42_RS33095 0.19 +0.0 2,832,954 + RR42_RS33095 0.23 +0.3 2,832,955 - RR42_RS33095 0.23 +0.3 2,832,955 - RR42_RS33095 0.23 +0.9 2,832,955 - RR42_RS33095 0.23 +0.7 2,833,132 + RR42_RS33095 0.42 -0.3 2,833,132 + RR42_RS33095 0.42 +1.0 2,833,132 + RR42_RS33095 0.42 -0.4 2,833,287 + RR42_RS33095 0.60 +0.2 2,833,287 + RR42_RS33095 0.60 -0.6 2,833,288 - RR42_RS33095 0.60 -1.4 2,833,288 - RR42_RS33095 0.60 -0.7 2,833,288 - RR42_RS33095 0.60 -0.4 2,833,288 - RR42_RS33095 0.60 +0.0 2,833,288 - RR42_RS33095 0.60 +0.2 2,833,356 + RR42_RS33095 0.67 +0.0 2,833,356 + RR42_RS33095 0.67 -0.9 2,833,356 + RR42_RS33095 0.67 +0.2 2,833,357 - RR42_RS33095 0.67 +0.7 2,833,357 - RR42_RS33095 0.67 +0.1 2,833,357 - RR42_RS33095 0.67 +0.5 2,833,357 - RR42_RS33095 0.67 +0.1
Or see this region's nucleotide sequence