Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32970 and RR42_RS32975 are separated by 152 nucleotides RR42_RS32975 and RR42_RS32980 are separated by 38 nucleotides
RR42_RS32970: RR42_RS32970 - benzoylformate decarboxylase, at 2,796,064 to 2,797,674
_RS32970
RR42_RS32975: RR42_RS32975 - LysR family transcriptional regulator, at 2,797,827 to 2,798,729
_RS32975
RR42_RS32980: RR42_RS32980 - pyruvate dehydrogenase, at 2,798,768 to 2,801,443
_RS32980
Position (kb)
2797
2798
2799 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2796.951 kb on - strand, within RR42_RS32970 at 2796.951 kb on - strand, within RR42_RS32970 at 2797.202 kb on + strand, within RR42_RS32970 at 2797.202 kb on + strand, within RR42_RS32970 at 2797.203 kb on - strand, within RR42_RS32970 at 2797.203 kb on - strand, within RR42_RS32970 at 2797.203 kb on - strand, within RR42_RS32970 at 2797.203 kb on - strand, within RR42_RS32970 at 2797.206 kb on - strand, within RR42_RS32970 at 2797.296 kb on + strand, within RR42_RS32970 at 2797.296 kb on + strand, within RR42_RS32970 at 2797.389 kb on + strand, within RR42_RS32970 at 2797.390 kb on - strand, within RR42_RS32970 at 2797.390 kb on - strand, within RR42_RS32970 at 2797.390 kb on - strand, within RR42_RS32970 at 2797.512 kb on - strand, within RR42_RS32970 at 2797.512 kb on - strand, within RR42_RS32970 at 2797.766 kb on + strand at 2797.766 kb on + strand at 2797.766 kb on + strand at 2797.767 kb on - strand at 2797.767 kb on - strand at 2797.784 kb on - strand at 2797.816 kb on + strand at 2798.334 kb on + strand, within RR42_RS32975 at 2798.335 kb on - strand, within RR42_RS32975 at 2798.640 kb on - strand at 2798.640 kb on - strand at 2798.769 kb on + strand at 2798.770 kb on - strand at 2798.770 kb on - strand at 2798.771 kb on + strand at 2798.772 kb on - strand at 2798.774 kb on - strand at 2798.892 kb on + strand at 2798.893 kb on - strand at 2798.953 kb on - strand at 2799.006 kb on + strand at 2799.006 kb on + strand at 2799.006 kb on + strand at 2799.007 kb on - strand at 2799.007 kb on - strand at 2799.007 kb on - strand at 2799.064 kb on + strand, within RR42_RS32980 at 2799.135 kb on + strand, within RR42_RS32980 at 2799.136 kb on - strand, within RR42_RS32980 at 2799.136 kb on - strand, within RR42_RS32980 at 2799.270 kb on - strand, within RR42_RS32980 at 2799.270 kb on - strand, within RR42_RS32980 at 2799.276 kb on + strand, within RR42_RS32980 at 2799.276 kb on + strand, within RR42_RS32980 at 2799.276 kb on + strand, within RR42_RS32980 at 2799.277 kb on - strand, within RR42_RS32980 at 2799.277 kb on - strand, within RR42_RS32980 at 2799.354 kb on + strand, within RR42_RS32980 at 2799.354 kb on + strand, within RR42_RS32980 at 2799.355 kb on - strand, within RR42_RS32980 at 2799.357 kb on + strand, within RR42_RS32980 at 2799.357 kb on + strand, within RR42_RS32980 at 2799.357 kb on + strand, within RR42_RS32980 at 2799.358 kb on - strand, within RR42_RS32980 at 2799.524 kb on - strand, within RR42_RS32980 at 2799.618 kb on + strand, within RR42_RS32980 at 2799.618 kb on + strand, within RR42_RS32980 at 2799.618 kb on + strand, within RR42_RS32980 at 2799.619 kb on - strand, within RR42_RS32980 at 2799.621 kb on + strand, within RR42_RS32980 at 2799.621 kb on + strand, within RR42_RS32980 at 2799.621 kb on + strand, within RR42_RS32980 at 2799.622 kb on - strand, within RR42_RS32980 at 2799.622 kb on - strand, within RR42_RS32980 at 2799.622 kb on - strand, within RR42_RS32980 at 2799.625 kb on + strand, within RR42_RS32980 at 2799.625 kb on + strand, within RR42_RS32980 at 2799.625 kb on + strand, within RR42_RS32980 at 2799.626 kb on - strand, within RR42_RS32980 at 2799.626 kb on - strand, within RR42_RS32980 at 2799.626 kb on - strand, within RR42_RS32980 at 2799.626 kb on - strand, within RR42_RS32980 at 2799.627 kb on + strand, within RR42_RS32980 at 2799.627 kb on + strand, within RR42_RS32980 at 2799.627 kb on + strand, within RR42_RS32980 at 2799.627 kb on + strand, within RR42_RS32980 at 2799.628 kb on - strand, within RR42_RS32980 at 2799.628 kb on - strand, within RR42_RS32980 at 2799.644 kb on - strand, within RR42_RS32980 at 2799.660 kb on + strand, within RR42_RS32980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,796,951 - RR42_RS32970 0.55 -0.4 2,796,951 - RR42_RS32970 0.55 +3.0 2,797,202 + RR42_RS32970 0.71 -0.5 2,797,202 + RR42_RS32970 0.71 -0.8 2,797,203 - RR42_RS32970 0.71 -1.6 2,797,203 - RR42_RS32970 0.71 -1.8 2,797,203 - RR42_RS32970 0.71 -0.7 2,797,203 - RR42_RS32970 0.71 -0.3 2,797,206 - RR42_RS32970 0.71 -0.5 2,797,296 + RR42_RS32970 0.76 -0.3 2,797,296 + RR42_RS32970 0.76 -1.3 2,797,389 + RR42_RS32970 0.82 +0.9 2,797,390 - RR42_RS32970 0.82 +0.3 2,797,390 - RR42_RS32970 0.82 -1.1 2,797,390 - RR42_RS32970 0.82 +0.3 2,797,512 - RR42_RS32970 0.90 +1.0 2,797,512 - RR42_RS32970 0.90 -1.3 2,797,766 + +0.1 2,797,766 + +0.2 2,797,766 + -0.5 2,797,767 - -0.6 2,797,767 - -0.3 2,797,784 - -0.5 2,797,816 + +1.5 2,798,334 + RR42_RS32975 0.56 -0.9 2,798,335 - RR42_RS32975 0.56 -1.0 2,798,640 - +0.1 2,798,640 - -1.3 2,798,769 + -0.8 2,798,770 - +0.7 2,798,770 - +0.6 2,798,771 + -1.3 2,798,772 - -1.1 2,798,774 - -1.2 2,798,892 + -0.1 2,798,893 - +0.1 2,798,953 - +0.2 2,799,006 + -0.1 2,799,006 + +0.4 2,799,006 + +0.7 2,799,007 - +1.4 2,799,007 - +0.2 2,799,007 - +0.1 2,799,064 + RR42_RS32980 0.11 +0.6 2,799,135 + RR42_RS32980 0.14 -0.1 2,799,136 - RR42_RS32980 0.14 -0.6 2,799,136 - RR42_RS32980 0.14 -0.6 2,799,270 - RR42_RS32980 0.19 -0.6 2,799,270 - RR42_RS32980 0.19 -0.6 2,799,276 + RR42_RS32980 0.19 -1.0 2,799,276 + RR42_RS32980 0.19 -0.5 2,799,276 + RR42_RS32980 0.19 -0.6 2,799,277 - RR42_RS32980 0.19 +0.3 2,799,277 - RR42_RS32980 0.19 -0.4 2,799,354 + RR42_RS32980 0.22 -0.1 2,799,354 + RR42_RS32980 0.22 -0.3 2,799,355 - RR42_RS32980 0.22 +0.3 2,799,357 + RR42_RS32980 0.22 +0.2 2,799,357 + RR42_RS32980 0.22 +0.0 2,799,357 + RR42_RS32980 0.22 +0.5 2,799,358 - RR42_RS32980 0.22 -0.3 2,799,524 - RR42_RS32980 0.28 +1.6 2,799,618 + RR42_RS32980 0.32 +0.7 2,799,618 + RR42_RS32980 0.32 -0.0 2,799,618 + RR42_RS32980 0.32 -1.6 2,799,619 - RR42_RS32980 0.32 -1.5 2,799,621 + RR42_RS32980 0.32 +1.0 2,799,621 + RR42_RS32980 0.32 -1.0 2,799,621 + RR42_RS32980 0.32 +0.1 2,799,622 - RR42_RS32980 0.32 -0.5 2,799,622 - RR42_RS32980 0.32 -0.4 2,799,622 - RR42_RS32980 0.32 +0.4 2,799,625 + RR42_RS32980 0.32 -0.5 2,799,625 + RR42_RS32980 0.32 -1.0 2,799,625 + RR42_RS32980 0.32 +0.1 2,799,626 - RR42_RS32980 0.32 +2.0 2,799,626 - RR42_RS32980 0.32 +1.7 2,799,626 - RR42_RS32980 0.32 +0.0 2,799,626 - RR42_RS32980 0.32 +0.2 2,799,627 + RR42_RS32980 0.32 +0.3 2,799,627 + RR42_RS32980 0.32 +2.7 2,799,627 + RR42_RS32980 0.32 -0.4 2,799,627 + RR42_RS32980 0.32 +0.4 2,799,628 - RR42_RS32980 0.32 +0.4 2,799,628 - RR42_RS32980 0.32 +0.3 2,799,644 - RR42_RS32980 0.33 +0.5 2,799,660 + RR42_RS32980 0.33 -0.3
Or see this region's nucleotide sequence