Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32735 and RR42_RS32740 are separated by 44 nucleotides RR42_RS32740 and RR42_RS32745 are separated by 109 nucleotides RR42_RS32745 and RR42_RS32750 are separated by 46 nucleotides
RR42_RS32735: RR42_RS32735 - 3-methylcrotonyl-CoA carboxylase, at 2,738,477 to 2,740,501
_RS32735
RR42_RS32740: RR42_RS32740 - enoyl-CoA hydratase, at 2,740,546 to 2,741,367
_RS32740
RR42_RS32745: RR42_RS32745 - acyl-CoA dehydrogenase, at 2,741,477 to 2,742,637
_RS32745
RR42_RS32750: RR42_RS32750 - acetyl-CoA carboxylase, at 2,742,684 to 2,744,303
_RS32750
Position (kb)
2741
2742
2743 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2740.523 kb on + strand at 2740.524 kb on - strand at 2741.106 kb on - strand, within RR42_RS32740 at 2741.413 kb on - strand at 2741.712 kb on + strand, within RR42_RS32745 at 2741.712 kb on + strand, within RR42_RS32745 at 2741.712 kb on + strand, within RR42_RS32745 at 2741.712 kb on + strand, within RR42_RS32745 at 2741.713 kb on - strand, within RR42_RS32745 at 2741.713 kb on - strand, within RR42_RS32745 at 2741.730 kb on + strand, within RR42_RS32745 at 2741.925 kb on + strand, within RR42_RS32745 at 2741.930 kb on - strand, within RR42_RS32745 at 2741.930 kb on - strand, within RR42_RS32745 at 2742.124 kb on + strand, within RR42_RS32745 at 2742.125 kb on - strand, within RR42_RS32745 at 2742.195 kb on + strand, within RR42_RS32745 at 2742.196 kb on - strand, within RR42_RS32745 at 2742.356 kb on - strand, within RR42_RS32745 at 2742.408 kb on + strand, within RR42_RS32745 at 2742.408 kb on + strand, within RR42_RS32745 at 2742.409 kb on - strand, within RR42_RS32745 at 2742.465 kb on + strand, within RR42_RS32745 at 2742.631 kb on - strand at 2742.631 kb on - strand at 2742.632 kb on + strand at 2742.632 kb on + strand at 2742.633 kb on - strand at 2742.633 kb on - strand at 2743.067 kb on - strand, within RR42_RS32750 at 2743.163 kb on + strand, within RR42_RS32750 at 2743.277 kb on + strand, within RR42_RS32750 at 2743.277 kb on + strand, within RR42_RS32750 at 2743.278 kb on - strand, within RR42_RS32750 at 2743.278 kb on - strand, within RR42_RS32750 at 2743.334 kb on - strand, within RR42_RS32750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,740,523 + -0.4 2,740,524 - -0.4 2,741,106 - RR42_RS32740 0.68 -3.4 2,741,413 - -0.7 2,741,712 + RR42_RS32745 0.20 -0.2 2,741,712 + RR42_RS32745 0.20 -1.0 2,741,712 + RR42_RS32745 0.20 +2.0 2,741,712 + RR42_RS32745 0.20 +0.3 2,741,713 - RR42_RS32745 0.20 +0.4 2,741,713 - RR42_RS32745 0.20 +0.7 2,741,730 + RR42_RS32745 0.22 +0.2 2,741,925 + RR42_RS32745 0.39 +0.8 2,741,930 - RR42_RS32745 0.39 -0.9 2,741,930 - RR42_RS32745 0.39 -0.2 2,742,124 + RR42_RS32745 0.56 -1.3 2,742,125 - RR42_RS32745 0.56 -1.5 2,742,195 + RR42_RS32745 0.62 -0.5 2,742,196 - RR42_RS32745 0.62 -1.0 2,742,356 - RR42_RS32745 0.76 -1.8 2,742,408 + RR42_RS32745 0.80 -0.6 2,742,408 + RR42_RS32745 0.80 +0.5 2,742,409 - RR42_RS32745 0.80 +1.0 2,742,465 + RR42_RS32745 0.85 -0.0 2,742,631 - +0.1 2,742,631 - -0.9 2,742,632 + +1.1 2,742,632 + +0.1 2,742,633 - -1.7 2,742,633 - -2.3 2,743,067 - RR42_RS32750 0.24 -0.6 2,743,163 + RR42_RS32750 0.30 +0.0 2,743,277 + RR42_RS32750 0.37 -0.8 2,743,277 + RR42_RS32750 0.37 -1.3 2,743,278 - RR42_RS32750 0.37 +1.7 2,743,278 - RR42_RS32750 0.37 -0.3 2,743,334 - RR42_RS32750 0.40 +1.1
Or see this region's nucleotide sequence