Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32600 and RR42_RS32605 are separated by 680 nucleotides RR42_RS32605 and RR42_RS32610 are separated by 122 nucleotides
RR42_RS32600: RR42_RS32600 - cytochrome O ubiquinol oxidase subunit II, at 2,709,779 to 2,710,747
_RS32600
RR42_RS32605: RR42_RS32605 - sulfonate ABC transporter substrate-binding protein, at 2,711,428 to 2,712,396
_RS32605
RR42_RS32610: RR42_RS32610 - O-acetylhomoserine aminocarboxypropyltransferase, at 2,712,519 to 2,713,793
_RS32610
Position (kb)
2711
2712
2713 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2710.739 kb on + strand at 2711.317 kb on + strand at 2711.331 kb on - strand at 2711.382 kb on - strand at 2711.382 kb on - strand at 2711.740 kb on + strand, within RR42_RS32605 at 2711.740 kb on + strand, within RR42_RS32605 at 2711.740 kb on + strand, within RR42_RS32605 at 2711.866 kb on + strand, within RR42_RS32605 at 2711.867 kb on - strand, within RR42_RS32605 at 2711.867 kb on - strand, within RR42_RS32605 at 2711.867 kb on - strand, within RR42_RS32605 at 2711.926 kb on + strand, within RR42_RS32605 at 2712.001 kb on + strand, within RR42_RS32605 at 2712.002 kb on - strand, within RR42_RS32605 at 2712.046 kb on + strand, within RR42_RS32605 at 2712.047 kb on - strand, within RR42_RS32605 at 2712.047 kb on - strand, within RR42_RS32605 at 2712.047 kb on - strand, within RR42_RS32605 at 2712.076 kb on + strand, within RR42_RS32605 at 2712.076 kb on + strand, within RR42_RS32605 at 2712.076 kb on + strand, within RR42_RS32605 at 2712.077 kb on - strand, within RR42_RS32605 at 2712.077 kb on - strand, within RR42_RS32605 at 2712.077 kb on - strand, within RR42_RS32605 at 2712.077 kb on - strand, within RR42_RS32605 at 2712.077 kb on - strand, within RR42_RS32605 at 2712.404 kb on + strand at 2712.405 kb on - strand at 2712.468 kb on + strand at 2712.468 kb on + strand at 2712.469 kb on - strand at 2712.489 kb on + strand at 2712.490 kb on - strand at 2712.490 kb on - strand at 2712.490 kb on - strand at 2712.490 kb on - strand at 2712.490 kb on - strand at 2712.493 kb on + strand at 2712.494 kb on - strand at 2712.594 kb on + strand at 2712.595 kb on - strand at 2712.668 kb on + strand, within RR42_RS32610 at 2712.669 kb on - strand, within RR42_RS32610 at 2712.672 kb on + strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.673 kb on - strand, within RR42_RS32610 at 2712.780 kb on + strand, within RR42_RS32610 at 2712.780 kb on + strand, within RR42_RS32610 at 2712.780 kb on + strand, within RR42_RS32610 at 2712.837 kb on + strand, within RR42_RS32610 at 2712.849 kb on + strand, within RR42_RS32610 at 2712.849 kb on + strand, within RR42_RS32610 at 2712.850 kb on - strand, within RR42_RS32610 at 2712.850 kb on - strand, within RR42_RS32610 at 2712.850 kb on - strand, within RR42_RS32610 at 2712.850 kb on - strand, within RR42_RS32610 at 2713.029 kb on - strand, within RR42_RS32610 at 2713.029 kb on - strand, within RR42_RS32610 at 2713.074 kb on + strand, within RR42_RS32610 at 2713.074 kb on + strand, within RR42_RS32610 at 2713.075 kb on - strand, within RR42_RS32610 at 2713.131 kb on + strand, within RR42_RS32610 at 2713.132 kb on - strand, within RR42_RS32610 at 2713.242 kb on + strand, within RR42_RS32610 at 2713.284 kb on + strand, within RR42_RS32610 at 2713.285 kb on - strand, within RR42_RS32610 at 2713.285 kb on - strand, within RR42_RS32610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,710,739 + +0.8 2,711,317 + +0.8 2,711,331 - +0.7 2,711,382 - -0.8 2,711,382 - +0.3 2,711,740 + RR42_RS32605 0.32 +0.2 2,711,740 + RR42_RS32605 0.32 -0.6 2,711,740 + RR42_RS32605 0.32 +0.1 2,711,866 + RR42_RS32605 0.45 -0.5 2,711,867 - RR42_RS32605 0.45 +0.3 2,711,867 - RR42_RS32605 0.45 +0.7 2,711,867 - RR42_RS32605 0.45 +0.3 2,711,926 + RR42_RS32605 0.51 -0.1 2,712,001 + RR42_RS32605 0.59 +0.7 2,712,002 - RR42_RS32605 0.59 -0.2 2,712,046 + RR42_RS32605 0.64 -1.8 2,712,047 - RR42_RS32605 0.64 -0.4 2,712,047 - RR42_RS32605 0.64 +0.5 2,712,047 - RR42_RS32605 0.64 +0.4 2,712,076 + RR42_RS32605 0.67 +0.0 2,712,076 + RR42_RS32605 0.67 +0.5 2,712,076 + RR42_RS32605 0.67 -0.2 2,712,077 - RR42_RS32605 0.67 +0.7 2,712,077 - RR42_RS32605 0.67 -1.6 2,712,077 - RR42_RS32605 0.67 +1.5 2,712,077 - RR42_RS32605 0.67 -1.0 2,712,077 - RR42_RS32605 0.67 +0.5 2,712,404 + +0.8 2,712,405 - +1.6 2,712,468 + +0.5 2,712,468 + +0.5 2,712,469 - +0.4 2,712,489 + -0.2 2,712,490 - -0.2 2,712,490 - -0.2 2,712,490 - +2.1 2,712,490 - -1.4 2,712,490 - -1.2 2,712,493 + +0.1 2,712,494 - -0.4 2,712,594 + -3.0 2,712,595 - +1.0 2,712,668 + RR42_RS32610 0.12 -0.4 2,712,669 - RR42_RS32610 0.12 +1.4 2,712,672 + RR42_RS32610 0.12 +0.5 2,712,673 - RR42_RS32610 0.12 +0.1 2,712,673 - RR42_RS32610 0.12 -0.2 2,712,673 - RR42_RS32610 0.12 -0.2 2,712,673 - RR42_RS32610 0.12 -0.6 2,712,673 - RR42_RS32610 0.12 +0.0 2,712,673 - RR42_RS32610 0.12 +0.2 2,712,780 + RR42_RS32610 0.20 +0.2 2,712,780 + RR42_RS32610 0.20 -2.1 2,712,780 + RR42_RS32610 0.20 +1.0 2,712,837 + RR42_RS32610 0.25 +0.3 2,712,849 + RR42_RS32610 0.26 -0.1 2,712,849 + RR42_RS32610 0.26 +1.6 2,712,850 - RR42_RS32610 0.26 +2.0 2,712,850 - RR42_RS32610 0.26 -0.9 2,712,850 - RR42_RS32610 0.26 -0.8 2,712,850 - RR42_RS32610 0.26 -0.7 2,713,029 - RR42_RS32610 0.40 +0.2 2,713,029 - RR42_RS32610 0.40 +0.5 2,713,074 + RR42_RS32610 0.44 +0.0 2,713,074 + RR42_RS32610 0.44 -0.7 2,713,075 - RR42_RS32610 0.44 -0.4 2,713,131 + RR42_RS32610 0.48 -0.3 2,713,132 - RR42_RS32610 0.48 -0.7 2,713,242 + RR42_RS32610 0.57 -0.4 2,713,284 + RR42_RS32610 0.60 +0.5 2,713,285 - RR42_RS32610 0.60 -1.4 2,713,285 - RR42_RS32610 0.60 -0.6
Or see this region's nucleotide sequence