Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32390 and RR42_RS32395 are separated by 219 nucleotides RR42_RS32395 and RR42_RS32400 are separated by 360 nucleotides
RR42_RS32390: RR42_RS32390 - citrate synthase, at 2,661,543 to 2,662,772
_RS32390
RR42_RS32395: RR42_RS32395 - IclR family transcriptional regulator, at 2,662,992 to 2,663,720
_RS32395
RR42_RS32400: RR42_RS32400 - hypothetical protein, at 2,664,081 to 2,664,551
_RS32400
Position (kb)
2662
2663
2664 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2662.225 kb on + strand, within RR42_RS32390 at 2662.225 kb on + strand, within RR42_RS32390 at 2662.226 kb on - strand, within RR42_RS32390 at 2662.226 kb on - strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.514 kb on - strand, within RR42_RS32390 at 2662.514 kb on - strand, within RR42_RS32390 at 2662.516 kb on + strand, within RR42_RS32390 at 2662.516 kb on + strand, within RR42_RS32390 at 2662.517 kb on - strand, within RR42_RS32390 at 2662.557 kb on - strand, within RR42_RS32390 at 2662.585 kb on + strand, within RR42_RS32390 at 2662.586 kb on - strand, within RR42_RS32390 at 2662.586 kb on - strand, within RR42_RS32390 at 2662.726 kb on + strand at 2662.727 kb on - strand at 2662.727 kb on - strand at 2662.727 kb on - strand at 2662.764 kb on + strand at 2662.764 kb on + strand at 2662.765 kb on - strand at 2662.789 kb on + strand at 2663.149 kb on - strand, within RR42_RS32395 at 2663.205 kb on + strand, within RR42_RS32395 at 2663.335 kb on + strand, within RR42_RS32395 at 2663.371 kb on + strand, within RR42_RS32395 at 2663.371 kb on + strand, within RR42_RS32395 at 2663.372 kb on - strand, within RR42_RS32395 at 2663.372 kb on - strand, within RR42_RS32395 at 2663.452 kb on + strand, within RR42_RS32395 at 2663.452 kb on + strand, within RR42_RS32395 at 2663.652 kb on + strand at 2663.711 kb on + strand at 2663.711 kb on + strand at 2663.712 kb on - strand at 2663.712 kb on - strand at 2663.712 kb on - strand at 2664.249 kb on - strand, within RR42_RS32400 at 2664.249 kb on - strand, within RR42_RS32400 at 2664.286 kb on + strand, within RR42_RS32400 at 2664.298 kb on + strand, within RR42_RS32400 at 2664.298 kb on + strand, within RR42_RS32400 at 2664.304 kb on + strand, within RR42_RS32400 at 2664.319 kb on + strand, within RR42_RS32400 at 2664.319 kb on + strand, within RR42_RS32400 at 2664.341 kb on + strand, within RR42_RS32400 at 2664.341 kb on + strand, within RR42_RS32400 at 2664.342 kb on - strand, within RR42_RS32400 at 2664.342 kb on - strand, within RR42_RS32400 at 2664.342 kb on - strand, within RR42_RS32400 at 2664.404 kb on - strand, within RR42_RS32400 at 2664.451 kb on + strand, within RR42_RS32400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,662,225 + RR42_RS32390 0.55 -0.0 2,662,225 + RR42_RS32390 0.55 +0.1 2,662,226 - RR42_RS32390 0.56 +0.5 2,662,226 - RR42_RS32390 0.56 +0.8 2,662,345 + RR42_RS32390 0.65 +1.0 2,662,345 + RR42_RS32390 0.65 +1.0 2,662,345 + RR42_RS32390 0.65 -1.3 2,662,345 + RR42_RS32390 0.65 -0.6 2,662,345 + RR42_RS32390 0.65 -0.6 2,662,514 - RR42_RS32390 0.79 +1.4 2,662,514 - RR42_RS32390 0.79 -0.7 2,662,516 + RR42_RS32390 0.79 -0.3 2,662,516 + RR42_RS32390 0.79 +1.9 2,662,517 - RR42_RS32390 0.79 -1.3 2,662,557 - RR42_RS32390 0.82 -0.8 2,662,585 + RR42_RS32390 0.85 +0.4 2,662,586 - RR42_RS32390 0.85 +0.9 2,662,586 - RR42_RS32390 0.85 -0.2 2,662,726 + -1.4 2,662,727 - +0.4 2,662,727 - +1.3 2,662,727 - +1.0 2,662,764 + -1.4 2,662,764 + +0.1 2,662,765 - -0.1 2,662,789 + -1.1 2,663,149 - RR42_RS32395 0.22 -0.7 2,663,205 + RR42_RS32395 0.29 +0.5 2,663,335 + RR42_RS32395 0.47 +2.0 2,663,371 + RR42_RS32395 0.52 +0.8 2,663,371 + RR42_RS32395 0.52 -1.2 2,663,372 - RR42_RS32395 0.52 -0.4 2,663,372 - RR42_RS32395 0.52 +0.3 2,663,452 + RR42_RS32395 0.63 +0.1 2,663,452 + RR42_RS32395 0.63 +1.3 2,663,652 + -2.0 2,663,711 + +0.3 2,663,711 + -0.9 2,663,712 - -0.6 2,663,712 - +0.3 2,663,712 - -0.6 2,664,249 - RR42_RS32400 0.36 -0.3 2,664,249 - RR42_RS32400 0.36 +1.1 2,664,286 + RR42_RS32400 0.44 +0.5 2,664,298 + RR42_RS32400 0.46 -0.4 2,664,298 + RR42_RS32400 0.46 -1.1 2,664,304 + RR42_RS32400 0.47 +0.4 2,664,319 + RR42_RS32400 0.51 -0.5 2,664,319 + RR42_RS32400 0.51 -0.7 2,664,341 + RR42_RS32400 0.55 +0.1 2,664,341 + RR42_RS32400 0.55 -0.4 2,664,342 - RR42_RS32400 0.55 -0.1 2,664,342 - RR42_RS32400 0.55 +1.1 2,664,342 - RR42_RS32400 0.55 -2.8 2,664,404 - RR42_RS32400 0.69 +1.0 2,664,451 + RR42_RS32400 0.79 -0.6
Or see this region's nucleotide sequence