Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32300

Experiment: R2A with Sisomicin 0.035 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS32295 and RR42_RS32300 are separated by 930 nucleotidesRR42_RS32300 and RR42_RS32305 are separated by 153 nucleotides RR42_RS32295: RR42_RS32295 - chemotaxis protein, at 2,633,341 to 2,634,897 _RS32295 RR42_RS32300: RR42_RS32300 - nickel transporter, at 2,635,828 to 2,636,886 _RS32300 RR42_RS32305: RR42_RS32305 - hypothetical protein, at 2,637,040 to 2,637,963 _RS32305 Position (kb) 2635 2636 2637Strain fitness (log2 ratio) -2 -1 0 1 2at 2634.885 kb on + strandat 2634.885 kb on + strandat 2635.046 kb on + strandat 2635.046 kb on + strandat 2635.046 kb on + strandat 2635.135 kb on + strandat 2635.135 kb on + strandat 2635.135 kb on + strandat 2635.135 kb on + strandat 2635.135 kb on + strandat 2635.136 kb on - strandat 2635.136 kb on - strandat 2635.136 kb on - strandat 2635.320 kb on + strandat 2635.841 kb on + strandat 2635.897 kb on + strandat 2635.897 kb on + strandat 2635.898 kb on - strandat 2636.289 kb on + strand, within RR42_RS32300at 2636.289 kb on + strand, within RR42_RS32300at 2636.290 kb on - strand, within RR42_RS32300at 2636.567 kb on + strand, within RR42_RS32300at 2636.639 kb on - strand, within RR42_RS32300at 2636.639 kb on - strand, within RR42_RS32300at 2636.639 kb on - strand, within RR42_RS32300at 2636.766 kb on + strand, within RR42_RS32300at 2636.791 kb on + strandat 2636.792 kb on - strandat 2636.858 kb on - strandat 2636.923 kb on + strandat 2636.924 kb on - strandat 2637.026 kb on + strandat 2637.026 kb on + strandat 2637.027 kb on - strandat 2637.027 kb on - strandat 2637.027 kb on - strandat 2637.095 kb on + strandat 2637.096 kb on - strandat 2637.124 kb on + strandat 2637.125 kb on - strandat 2637.125 kb on - strandat 2637.198 kb on + strand, within RR42_RS32305at 2637.198 kb on + strand, within RR42_RS32305at 2637.199 kb on - strand, within RR42_RS32305at 2637.199 kb on - strand, within RR42_RS32305at 2637.199 kb on - strand, within RR42_RS32305at 2637.204 kb on - strand, within RR42_RS32305at 2637.230 kb on - strand, within RR42_RS32305at 2637.230 kb on - strand, within RR42_RS32305at 2637.242 kb on + strand, within RR42_RS32305at 2637.243 kb on - strand, within RR42_RS32305at 2637.243 kb on - strand, within RR42_RS32305at 2637.260 kb on + strand, within RR42_RS32305at 2637.270 kb on - strand, within RR42_RS32305at 2637.278 kb on - strand, within RR42_RS32305at 2637.340 kb on + strand, within RR42_RS32305at 2637.419 kb on - strand, within RR42_RS32305at 2637.551 kb on - strand, within RR42_RS32305at 2637.551 kb on - strand, within RR42_RS32305at 2637.662 kb on + strand, within RR42_RS32305at 2637.843 kb on + strand, within RR42_RS32305at 2637.843 kb on + strand, within RR42_RS32305at 2637.847 kb on + strand, within RR42_RS32305at 2637.848 kb on - strand, within RR42_RS32305at 2637.848 kb on - strand, within RR42_RS32305

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml
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2,634,885 + -0.0
2,634,885 + +1.4
2,635,046 + +0.7
2,635,046 + -0.0
2,635,046 + -1.0
2,635,135 + +1.5
2,635,135 + +1.4
2,635,135 + +2.5
2,635,135 + -0.2
2,635,135 + -0.4
2,635,136 - +0.1
2,635,136 - -0.2
2,635,136 - -2.3
2,635,320 + -0.3
2,635,841 + +0.7
2,635,897 + +0.2
2,635,897 + +0.3
2,635,898 - -0.3
2,636,289 + RR42_RS32300 0.44 +1.0
2,636,289 + RR42_RS32300 0.44 -0.3
2,636,290 - RR42_RS32300 0.44 -0.9
2,636,567 + RR42_RS32300 0.70 +0.9
2,636,639 - RR42_RS32300 0.77 -0.8
2,636,639 - RR42_RS32300 0.77 -0.3
2,636,639 - RR42_RS32300 0.77 -0.0
2,636,766 + RR42_RS32300 0.89 +1.1
2,636,791 + -1.3
2,636,792 - -0.8
2,636,858 - +1.0
2,636,923 + -1.0
2,636,924 - -0.5
2,637,026 + +2.0
2,637,026 + -1.0
2,637,027 - -1.0
2,637,027 - +0.7
2,637,027 - -0.0
2,637,095 + -0.4
2,637,096 - -0.9
2,637,124 + -0.5
2,637,125 - +0.8
2,637,125 - +0.1
2,637,198 + RR42_RS32305 0.17 -1.2
2,637,198 + RR42_RS32305 0.17 -0.8
2,637,199 - RR42_RS32305 0.17 +0.4
2,637,199 - RR42_RS32305 0.17 +1.0
2,637,199 - RR42_RS32305 0.17 +0.5
2,637,204 - RR42_RS32305 0.18 -0.2
2,637,230 - RR42_RS32305 0.21 +0.3
2,637,230 - RR42_RS32305 0.21 +0.5
2,637,242 + RR42_RS32305 0.22 -0.1
2,637,243 - RR42_RS32305 0.22 +0.6
2,637,243 - RR42_RS32305 0.22 +1.3
2,637,260 + RR42_RS32305 0.24 +0.9
2,637,270 - RR42_RS32305 0.25 +0.1
2,637,278 - RR42_RS32305 0.26 -0.6
2,637,340 + RR42_RS32305 0.32 +0.0
2,637,419 - RR42_RS32305 0.41 +0.3
2,637,551 - RR42_RS32305 0.55 +0.1
2,637,551 - RR42_RS32305 0.55 -0.9
2,637,662 + RR42_RS32305 0.67 +1.0
2,637,843 + RR42_RS32305 0.87 +0.6
2,637,843 + RR42_RS32305 0.87 +0.1
2,637,847 + RR42_RS32305 0.87 -0.0
2,637,848 - RR42_RS32305 0.87 -0.2
2,637,848 - RR42_RS32305 0.87 -0.0

Or see this region's nucleotide sequence