Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32235 and RR42_RS32240 are separated by 235 nucleotides RR42_RS32240 and RR42_RS32245 are separated by 281 nucleotides
RR42_RS32235: RR42_RS32235 - hopanoid biosynthesis associated radical SAM protein HpnH, at 2,618,430 to 2,619,596
_RS32235
RR42_RS32240: RR42_RS32240 - ABC transporter, at 2,619,832 to 2,620,479
_RS32240
RR42_RS32245: RR42_RS32245 - RND transporter, at 2,620,761 to 2,623,430
_RS32245
Position (kb)
2619
2620
2621 Strain fitness (log2 ratio)
-2
-1
0
1 at 2618.872 kb on - strand, within RR42_RS32235 at 2619.030 kb on + strand, within RR42_RS32235 at 2619.186 kb on + strand, within RR42_RS32235 at 2619.187 kb on - strand, within RR42_RS32235 at 2619.187 kb on - strand, within RR42_RS32235 at 2619.246 kb on + strand, within RR42_RS32235 at 2619.246 kb on + strand, within RR42_RS32235 at 2619.265 kb on - strand, within RR42_RS32235 at 2619.312 kb on + strand, within RR42_RS32235 at 2619.313 kb on - strand, within RR42_RS32235 at 2619.357 kb on - strand, within RR42_RS32235 at 2619.438 kb on - strand, within RR42_RS32235 at 2619.462 kb on - strand, within RR42_RS32235 at 2619.492 kb on + strand at 2619.493 kb on - strand at 2619.493 kb on - strand at 2619.759 kb on - strand at 2619.872 kb on + strand at 2619.873 kb on - strand at 2619.873 kb on - strand at 2619.873 kb on - strand at 2619.909 kb on + strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.930 kb on - strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.968 kb on + strand, within RR42_RS32240 at 2619.969 kb on - strand, within RR42_RS32240 at 2619.969 kb on - strand, within RR42_RS32240 at 2620.092 kb on - strand, within RR42_RS32240 at 2620.092 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.140 kb on - strand, within RR42_RS32240 at 2620.172 kb on + strand, within RR42_RS32240 at 2620.173 kb on - strand, within RR42_RS32240 at 2620.618 kb on + strand at 2620.619 kb on - strand at 2620.620 kb on + strand at 2620.620 kb on + strand at 2620.620 kb on + strand at 2620.620 kb on + strand at 2620.620 kb on + strand at 2620.621 kb on - strand at 2620.621 kb on - strand at 2620.671 kb on - strand at 2620.673 kb on - strand at 2620.673 kb on - strand at 2620.673 kb on - strand at 2620.751 kb on + strand at 2620.751 kb on + strand at 2620.751 kb on + strand at 2620.751 kb on + strand at 2620.751 kb on + strand at 2620.751 kb on + strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.752 kb on - strand at 2620.860 kb on + strand at 2620.860 kb on + strand at 2620.861 kb on - strand at 2620.861 kb on - strand at 2620.861 kb on - strand at 2620.861 kb on - strand at 2620.893 kb on - strand at 2621.124 kb on + strand, within RR42_RS32245 at 2621.229 kb on - strand, within RR42_RS32245 at 2621.442 kb on + strand, within RR42_RS32245 at 2621.442 kb on + strand, within RR42_RS32245 at 2621.442 kb on + strand, within RR42_RS32245 at 2621.443 kb on - strand, within RR42_RS32245 at 2621.443 kb on - strand, within RR42_RS32245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,618,872 - RR42_RS32235 0.38 -1.5 2,619,030 + RR42_RS32235 0.51 -2.4 2,619,186 + RR42_RS32235 0.65 -1.5 2,619,187 - RR42_RS32235 0.65 -1.4 2,619,187 - RR42_RS32235 0.65 +1.6 2,619,246 + RR42_RS32235 0.70 -0.9 2,619,246 + RR42_RS32235 0.70 -0.2 2,619,265 - RR42_RS32235 0.72 -0.8 2,619,312 + RR42_RS32235 0.76 -0.2 2,619,313 - RR42_RS32235 0.76 -1.6 2,619,357 - RR42_RS32235 0.79 -1.3 2,619,438 - RR42_RS32235 0.86 -1.0 2,619,462 - RR42_RS32235 0.88 -0.4 2,619,492 + -0.6 2,619,493 - +0.4 2,619,493 - -1.1 2,619,759 - -0.3 2,619,872 + -0.1 2,619,873 - -1.3 2,619,873 - +0.2 2,619,873 - -0.5 2,619,909 + RR42_RS32240 0.12 -2.5 2,619,930 - RR42_RS32240 0.15 -2.2 2,619,930 - RR42_RS32240 0.15 -0.8 2,619,930 - RR42_RS32240 0.15 -0.0 2,619,930 - RR42_RS32240 0.15 +1.2 2,619,968 + RR42_RS32240 0.21 -0.6 2,619,968 + RR42_RS32240 0.21 -1.0 2,619,968 + RR42_RS32240 0.21 -0.1 2,619,968 + RR42_RS32240 0.21 -0.0 2,619,969 - RR42_RS32240 0.21 +0.5 2,619,969 - RR42_RS32240 0.21 -1.5 2,620,092 - RR42_RS32240 0.40 -0.3 2,620,092 - RR42_RS32240 0.40 -0.9 2,620,140 - RR42_RS32240 0.48 -0.7 2,620,140 - RR42_RS32240 0.48 -1.6 2,620,140 - RR42_RS32240 0.48 -0.3 2,620,140 - RR42_RS32240 0.48 -1.6 2,620,140 - RR42_RS32240 0.48 -1.7 2,620,172 + RR42_RS32240 0.52 -0.7 2,620,173 - RR42_RS32240 0.53 +0.1 2,620,618 + -0.8 2,620,619 - -1.8 2,620,620 + +0.0 2,620,620 + -0.0 2,620,620 + -0.1 2,620,620 + -2.3 2,620,620 + -0.5 2,620,621 - +0.4 2,620,621 - +0.7 2,620,671 - -0.5 2,620,673 - -0.6 2,620,673 - +0.1 2,620,673 - -0.3 2,620,751 + +0.4 2,620,751 + -0.3 2,620,751 + -0.2 2,620,751 + -0.2 2,620,751 + -0.2 2,620,751 + +0.3 2,620,752 - -2.3 2,620,752 - -0.9 2,620,752 - +0.1 2,620,752 - +0.1 2,620,752 - -0.2 2,620,752 - -0.2 2,620,752 - +0.8 2,620,752 - -0.7 2,620,752 - -1.0 2,620,752 - -0.4 2,620,860 + -1.3 2,620,860 + -1.0 2,620,861 - -1.2 2,620,861 - -0.6 2,620,861 - -0.3 2,620,861 - -0.6 2,620,893 - -1.0 2,621,124 + RR42_RS32245 0.14 -0.8 2,621,229 - RR42_RS32245 0.18 -1.7 2,621,442 + RR42_RS32245 0.26 -2.2 2,621,442 + RR42_RS32245 0.26 -1.1 2,621,442 + RR42_RS32245 0.26 +0.3 2,621,443 - RR42_RS32245 0.26 +0.1 2,621,443 - RR42_RS32245 0.26 -0.6
Or see this region's nucleotide sequence