Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32225

Experiment: R2A with Sisomicin 0.035 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Sisomicin 0.035 mg/ml
2,615,302	+		RR42_RS32220	0.20	+1.3
2,615,302	+		RR42_RS32220	0.20	-0.8
2,615,302	+		RR42_RS32220	0.20	-0.5
2,615,303	-		RR42_RS32220	0.20	-0.9
2,615,303	-		RR42_RS32220	0.20	-0.1
2,615,475	+		RR42_RS32220	0.35	+0.6
2,615,779	+		RR42_RS32220	0.62	+0.5
2,615,780	-		RR42_RS32220	0.62	-0.8
2,615,901	-		RR42_RS32220	0.73	-1.6
2,615,916	-		RR42_RS32220	0.74	-0.5
2,615,916	-		RR42_RS32220	0.74	-0.9
2,615,964	-		RR42_RS32220	0.78	-0.5
2,616,048	-		RR42_RS32220	0.86	+0.4
2,616,048	-		RR42_RS32220	0.86	-0.7
2,616,069	-		RR42_RS32220	0.87	+4.1
2,616,119	+				-1.0
2,616,309	-				-1.4
2,616,314	-				+1.2
2,616,545	+		RR42_RS32225	0.31	-0.5
2,616,545	+		RR42_RS32225	0.31	-0.5
2,616,545	+		RR42_RS32225	0.31	+0.8
2,616,545	+		RR42_RS32225	0.31	-0.5
2,616,545	+		RR42_RS32225	0.31	-0.8
2,616,545	+		RR42_RS32225	0.31	-1.4
2,616,545	+		RR42_RS32225	0.31	-0.1
2,616,545	+		RR42_RS32225	0.31	-0.4
2,616,546	-		RR42_RS32225	0.31	+0.5
2,616,546	-		RR42_RS32225	0.31	-0.4
2,616,546	-		RR42_RS32225	0.31	-0.1
2,616,583	-		RR42_RS32225	0.35	-0.6
2,616,583	-		RR42_RS32225	0.35	+0.1
2,616,583	-		RR42_RS32225	0.35	-0.2
2,616,583	-		RR42_RS32225	0.35	-1.4
2,616,583	-		RR42_RS32225	0.35	+0.4
2,616,664	+		RR42_RS32225	0.43	-0.3
2,616,664	+		RR42_RS32225	0.43	-0.4
2,616,665	-		RR42_RS32225	0.43	-0.9
2,616,804	-		RR42_RS32225	0.57	-0.8
2,616,896	+		RR42_RS32225	0.66	+0.0
2,616,897	-		RR42_RS32225	0.66	+0.9
2,616,897	-		RR42_RS32225	0.66	-1.3
2,616,897	-		RR42_RS32225	0.66	+1.2
2,617,002	-		RR42_RS32225	0.77	+1.0
2,617,025	+		RR42_RS32225	0.79	+0.7
2,617,025	+		RR42_RS32225	0.79	-0.6
2,617,025	+		RR42_RS32225	0.79	-1.1
2,617,026	-		RR42_RS32225	0.79	-1.5
2,617,026	-		RR42_RS32225	0.79	-0.5
2,617,220	+				-0.6
2,617,236	+				+0.3
2,617,237	-				-0.9
2,617,237	-				+0.6
2,617,308	+				-0.8
2,617,308	+				-0.4
2,617,309	-				+0.9
2,617,310	+				-0.4
2,617,310	+				-0.0
2,617,310	+				+0.3
2,617,310	+				-0.3
2,617,312	+				-0.4
2,617,312	+				-0.6
2,617,312	+				+0.3
2,617,312	+				+0.2
2,617,312	+				+0.1
2,617,312	+				+0.1
2,617,312	+				+0.3
2,617,313	-				+0.6
2,617,313	-				+1.2
2,617,313	-				-0.5
2,617,313	-				+1.4
2,617,315	+				+0.6
2,617,315	+				-0.7
2,617,316	-				+0.5
2,617,348	+				-1.9
2,617,356	+				+1.8
2,617,357	-				+0.4
2,617,357	-				+0.3
2,617,388	+				+1.0
2,617,388	+				-0.5
2,617,389	-				-0.1
2,617,389	-				-0.8
2,617,394	+				-0.8
2,617,395	-				-0.4
2,617,459	+				-1.2
2,617,460	-				+0.7
2,617,460	-				-0.1
2,617,719	+		RR42_RS32230	0.32	-1.5
2,617,720	-		RR42_RS32230	0.32	-1.6
2,617,873	-		RR42_RS32230	0.48	-0.8
2,617,887	-		RR42_RS32230	0.49	-0.3
2,617,887	-		RR42_RS32230	0.49	-0.0
2,617,887	-		RR42_RS32230	0.49	-1.7
2,617,910	+		RR42_RS32230	0.51	-0.1
2,617,911	-		RR42_RS32230	0.51	-1.5
2,617,911	-		RR42_RS32230	0.51	+0.4
2,617,911	-		RR42_RS32230	0.51	+0.4
2,617,911	-		RR42_RS32230	0.51	+0.1
2,617,913	+		RR42_RS32230	0.52	-1.3
2,617,913	+		RR42_RS32230	0.52	+0.3
2,617,914	-		RR42_RS32230	0.52	-0.0
2,617,914	-		RR42_RS32230	0.52	+0.2
2,617,915	+		RR42_RS32230	0.52	-0.3
2,617,915	+		RR42_RS32230	0.52	+0.3
2,617,915	+		RR42_RS32230	0.52	+0.6
2,617,916	-		RR42_RS32230	0.52	-0.7
2,617,916	-		RR42_RS32230	0.52	-0.8
2,617,943	+		RR42_RS32230	0.55	+0.6
2,617,977	+		RR42_RS32230	0.58	+0.8
2,618,058	-		RR42_RS32230	0.66	-1.0
2,618,058	-		RR42_RS32230	0.66	-1.4
2,618,112	+		RR42_RS32230	0.72	-0.8