Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32105 and RR42_RS32110 are separated by 399 nucleotides RR42_RS32110 and RR42_RS32115 are separated by 100 nucleotides RR42_RS32115 and RR42_RS32120 are separated by 57 nucleotides
RR42_RS32105: RR42_RS32105 - hypothetical protein, at 2,587,153 to 2,587,671
_RS32105
RR42_RS32110: RR42_RS32110 - peptide ABC transporter permease, at 2,588,071 to 2,589,822
_RS32110
RR42_RS32115: RR42_RS32115 - curli production assembly protein CsgG, at 2,589,923 to 2,590,603
_RS32115
RR42_RS32120: RR42_RS32120 - lipoprotein, at 2,590,661 to 2,591,008
_RS32120
Position (kb)
2588
2589
2590 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2587.123 kb on + strand at 2587.175 kb on + strand at 2587.176 kb on - strand at 2587.504 kb on + strand, within RR42_RS32105 at 2587.504 kb on + strand, within RR42_RS32105 at 2587.505 kb on - strand, within RR42_RS32105 at 2587.573 kb on - strand, within RR42_RS32105 at 2587.573 kb on - strand, within RR42_RS32105 at 2587.573 kb on - strand, within RR42_RS32105 at 2587.599 kb on - strand, within RR42_RS32105 at 2587.629 kb on + strand at 2587.629 kb on + strand at 2587.694 kb on + strand at 2587.750 kb on + strand at 2587.750 kb on + strand at 2588.001 kb on - strand at 2588.001 kb on - strand at 2588.001 kb on - strand at 2588.001 kb on - strand at 2588.001 kb on - strand at 2588.001 kb on - strand at 2588.001 kb on - strand at 2588.003 kb on - strand at 2588.003 kb on - strand at 2588.010 kb on + strand at 2588.011 kb on - strand at 2588.011 kb on - strand at 2588.055 kb on + strand at 2588.055 kb on + strand at 2588.056 kb on - strand at 2588.506 kb on + strand, within RR42_RS32110 at 2588.972 kb on - strand, within RR42_RS32110 at 2588.972 kb on - strand, within RR42_RS32110 at 2589.143 kb on - strand, within RR42_RS32110 at 2589.251 kb on - strand, within RR42_RS32110 at 2589.479 kb on - strand, within RR42_RS32110 at 2589.820 kb on + strand at 2589.821 kb on - strand at 2589.892 kb on + strand at 2589.892 kb on + strand at 2589.893 kb on - strand at 2589.902 kb on + strand at 2589.902 kb on + strand at 2589.903 kb on - strand at 2589.949 kb on - strand at 2590.046 kb on + strand, within RR42_RS32115 at 2590.047 kb on - strand, within RR42_RS32115 at 2590.050 kb on + strand, within RR42_RS32115 at 2590.051 kb on - strand, within RR42_RS32115 at 2590.100 kb on + strand, within RR42_RS32115 at 2590.100 kb on + strand, within RR42_RS32115 at 2590.101 kb on - strand, within RR42_RS32115 at 2590.101 kb on - strand, within RR42_RS32115 at 2590.102 kb on + strand, within RR42_RS32115 at 2590.102 kb on + strand, within RR42_RS32115 at 2590.103 kb on - strand, within RR42_RS32115 at 2590.277 kb on + strand, within RR42_RS32115 at 2590.278 kb on - strand, within RR42_RS32115 at 2590.278 kb on - strand, within RR42_RS32115 at 2590.374 kb on - strand, within RR42_RS32115 at 2590.374 kb on - strand, within RR42_RS32115 at 2590.473 kb on + strand, within RR42_RS32115 at 2590.473 kb on + strand, within RR42_RS32115 at 2590.473 kb on + strand, within RR42_RS32115 at 2590.496 kb on + strand, within RR42_RS32115 at 2590.497 kb on - strand, within RR42_RS32115 at 2590.497 kb on - strand, within RR42_RS32115 at 2590.693 kb on - strand at 2590.693 kb on - strand at 2590.727 kb on + strand, within RR42_RS32120 at 2590.742 kb on + strand, within RR42_RS32120 at 2590.743 kb on - strand, within RR42_RS32120 at 2590.763 kb on + strand, within RR42_RS32120 at 2590.764 kb on - strand, within RR42_RS32120
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,587,123 + +0.6 2,587,175 + +1.0 2,587,176 - +0.2 2,587,504 + RR42_RS32105 0.68 -0.6 2,587,504 + RR42_RS32105 0.68 +0.4 2,587,505 - RR42_RS32105 0.68 -0.4 2,587,573 - RR42_RS32105 0.81 -0.9 2,587,573 - RR42_RS32105 0.81 -0.6 2,587,573 - RR42_RS32105 0.81 +0.6 2,587,599 - RR42_RS32105 0.86 -0.6 2,587,629 + +1.4 2,587,629 + -1.6 2,587,694 + -0.6 2,587,750 + -1.8 2,587,750 + +1.0 2,588,001 - -0.1 2,588,001 - -0.7 2,588,001 - +0.2 2,588,001 - +0.5 2,588,001 - -0.0 2,588,001 - +0.7 2,588,001 - -0.4 2,588,003 - +0.8 2,588,003 - -0.4 2,588,010 + -2.9 2,588,011 - +0.8 2,588,011 - -0.2 2,588,055 + -0.2 2,588,055 + +0.7 2,588,056 - +0.5 2,588,506 + RR42_RS32110 0.25 +1.3 2,588,972 - RR42_RS32110 0.51 -0.7 2,588,972 - RR42_RS32110 0.51 -1.0 2,589,143 - RR42_RS32110 0.61 +0.5 2,589,251 - RR42_RS32110 0.67 -0.2 2,589,479 - RR42_RS32110 0.80 +0.1 2,589,820 + -0.9 2,589,821 - -0.7 2,589,892 + -0.1 2,589,892 + +1.5 2,589,893 - +0.3 2,589,902 + -0.2 2,589,902 + -0.6 2,589,903 - +0.6 2,589,949 - -0.6 2,590,046 + RR42_RS32115 0.18 +1.0 2,590,047 - RR42_RS32115 0.18 +0.7 2,590,050 + RR42_RS32115 0.19 -0.4 2,590,051 - RR42_RS32115 0.19 -0.3 2,590,100 + RR42_RS32115 0.26 -0.1 2,590,100 + RR42_RS32115 0.26 +0.9 2,590,101 - RR42_RS32115 0.26 +0.2 2,590,101 - RR42_RS32115 0.26 -0.8 2,590,102 + RR42_RS32115 0.26 +0.1 2,590,102 + RR42_RS32115 0.26 -0.9 2,590,103 - RR42_RS32115 0.26 -0.8 2,590,277 + RR42_RS32115 0.52 -1.4 2,590,278 - RR42_RS32115 0.52 +1.1 2,590,278 - RR42_RS32115 0.52 -1.9 2,590,374 - RR42_RS32115 0.66 +1.1 2,590,374 - RR42_RS32115 0.66 +0.4 2,590,473 + RR42_RS32115 0.81 +2.2 2,590,473 + RR42_RS32115 0.81 +0.1 2,590,473 + RR42_RS32115 0.81 +1.2 2,590,496 + RR42_RS32115 0.84 -0.0 2,590,497 - RR42_RS32115 0.84 +0.4 2,590,497 - RR42_RS32115 0.84 -2.2 2,590,693 - -1.4 2,590,693 - -0.5 2,590,727 + RR42_RS32120 0.19 +0.4 2,590,742 + RR42_RS32120 0.23 +0.3 2,590,743 - RR42_RS32120 0.24 -0.5 2,590,763 + RR42_RS32120 0.29 +1.5 2,590,764 - RR42_RS32120 0.30 -0.3
Or see this region's nucleotide sequence