Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32000 and RR42_RS32005 are separated by 19 nucleotides RR42_RS32005 and RR42_RS32010 are separated by 121 nucleotides
RR42_RS32000: RR42_RS32000 - 3-oxosteroid 1-dehydrogenase, at 2,556,900 to 2,558,642
_RS32000
RR42_RS32005: RR42_RS32005 - flavin-dependent monooxygenase, at 2,558,662 to 2,559,855
_RS32005
RR42_RS32010: RR42_RS32010 - 5,10-methylene tetrahydromethanopterin reductase, at 2,559,977 to 2,561,128
_RS32010
Position (kb)
2558
2559
2560 Strain fitness (log2 ratio)
-1
0
1 at 2557.766 kb on - strand, within RR42_RS32000 at 2557.998 kb on - strand, within RR42_RS32000 at 2557.998 kb on - strand, within RR42_RS32000 at 2558.291 kb on - strand, within RR42_RS32000 at 2558.318 kb on - strand, within RR42_RS32000 at 2558.348 kb on - strand, within RR42_RS32000 at 2558.416 kb on + strand, within RR42_RS32000 at 2558.417 kb on - strand, within RR42_RS32000 at 2558.668 kb on - strand at 2558.741 kb on + strand at 2558.799 kb on - strand, within RR42_RS32005 at 2558.799 kb on - strand, within RR42_RS32005 at 2559.068 kb on + strand, within RR42_RS32005 at 2559.068 kb on + strand, within RR42_RS32005 at 2559.158 kb on + strand, within RR42_RS32005 at 2559.318 kb on - strand, within RR42_RS32005 at 2559.347 kb on + strand, within RR42_RS32005 at 2559.489 kb on + strand, within RR42_RS32005 at 2559.491 kb on + strand, within RR42_RS32005 at 2559.492 kb on - strand, within RR42_RS32005 at 2559.510 kb on + strand, within RR42_RS32005 at 2559.593 kb on + strand, within RR42_RS32005 at 2559.594 kb on - strand, within RR42_RS32005 at 2559.840 kb on - strand at 2559.944 kb on + strand at 2560.326 kb on + strand, within RR42_RS32010 at 2560.333 kb on - strand, within RR42_RS32010 at 2560.480 kb on - strand, within RR42_RS32010 at 2560.491 kb on + strand, within RR42_RS32010 at 2560.602 kb on + strand, within RR42_RS32010 at 2560.603 kb on - strand, within RR42_RS32010 at 2560.665 kb on + strand, within RR42_RS32010
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,557,766 - RR42_RS32000 0.50 -1.1 2,557,998 - RR42_RS32000 0.63 -0.6 2,557,998 - RR42_RS32000 0.63 -1.5 2,558,291 - RR42_RS32000 0.80 -0.4 2,558,318 - RR42_RS32000 0.81 -0.9 2,558,348 - RR42_RS32000 0.83 +1.6 2,558,416 + RR42_RS32000 0.87 -0.1 2,558,417 - RR42_RS32000 0.87 +0.4 2,558,668 - +0.6 2,558,741 + -0.2 2,558,799 - RR42_RS32005 0.11 +0.4 2,558,799 - RR42_RS32005 0.11 -0.9 2,559,068 + RR42_RS32005 0.34 -0.5 2,559,068 + RR42_RS32005 0.34 -0.6 2,559,158 + RR42_RS32005 0.42 -0.6 2,559,318 - RR42_RS32005 0.55 -0.4 2,559,347 + RR42_RS32005 0.57 +0.4 2,559,489 + RR42_RS32005 0.69 -0.4 2,559,491 + RR42_RS32005 0.69 +0.1 2,559,492 - RR42_RS32005 0.70 -0.6 2,559,510 + RR42_RS32005 0.71 -0.4 2,559,593 + RR42_RS32005 0.78 -0.1 2,559,594 - RR42_RS32005 0.78 -0.2 2,559,840 - +0.3 2,559,944 + +0.0 2,560,326 + RR42_RS32010 0.30 -0.7 2,560,333 - RR42_RS32010 0.31 +1.4 2,560,480 - RR42_RS32010 0.44 -1.1 2,560,491 + RR42_RS32010 0.45 -0.4 2,560,602 + RR42_RS32010 0.54 -0.4 2,560,603 - RR42_RS32010 0.54 -1.6 2,560,665 + RR42_RS32010 0.60 -0.3
Or see this region's nucleotide sequence