Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS31990

Experiment: R2A with Sisomicin 0.035 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31980 and RR42_RS31985 are separated by 11 nucleotidesRR42_RS31985 and RR42_RS31990 overlap by 17 nucleotidesRR42_RS31990 and RR42_RS31995 are separated by 99 nucleotidesRR42_RS31995 and RR42_RS32000 are separated by 58 nucleotides RR42_RS31980: RR42_RS31980 - 3-ketosteroid-9-alpha-hydroxylase, at 2,552,726 to 2,553,754 _RS31980 RR42_RS31985: RR42_RS31985 - short-chain dehydrogenase, at 2,553,766 to 2,554,533 _RS31985 RR42_RS31990: RR42_RS31990 - delta 4, 5-alpha steroid dehydrogenase, at 2,554,517 to 2,556,223 _RS31990 RR42_RS31995: RR42_RS31995 - flavin reductase, at 2,556,323 to 2,556,841 _RS31995 RR42_RS32000: RR42_RS32000 - 3-oxosteroid 1-dehydrogenase, at 2,556,900 to 2,558,642 _RS32000 Position (kb) 2554 2555 2556 2557Strain fitness (log2 ratio) -2 -1 0 1at 2554.011 kb on - strand, within RR42_RS31985at 2554.011 kb on - strand, within RR42_RS31985at 2554.080 kb on - strand, within RR42_RS31985at 2554.126 kb on - strand, within RR42_RS31985at 2554.126 kb on - strand, within RR42_RS31985at 2554.126 kb on - strand, within RR42_RS31985at 2554.860 kb on + strand, within RR42_RS31990at 2554.941 kb on + strand, within RR42_RS31990at 2554.941 kb on + strand, within RR42_RS31990at 2554.942 kb on - strand, within RR42_RS31990at 2554.942 kb on - strand, within RR42_RS31990at 2554.942 kb on - strand, within RR42_RS31990at 2554.942 kb on - strand, within RR42_RS31990at 2555.370 kb on + strand, within RR42_RS31990at 2555.410 kb on + strand, within RR42_RS31990at 2555.411 kb on - strand, within RR42_RS31990at 2555.808 kb on + strand, within RR42_RS31990at 2555.812 kb on - strand, within RR42_RS31990at 2555.817 kb on + strand, within RR42_RS31990at 2555.818 kb on - strand, within RR42_RS31990at 2555.818 kb on - strand, within RR42_RS31990at 2555.974 kb on - strand, within RR42_RS31990at 2556.043 kb on - strand, within RR42_RS31990at 2556.378 kb on + strand, within RR42_RS31995at 2556.378 kb on + strand, within RR42_RS31995at 2556.545 kb on - strand, within RR42_RS31995at 2556.805 kb on + strandat 2556.806 kb on - strandat 2556.829 kb on + strandat 2556.830 kb on - strandat 2556.858 kb on - strandat 2556.858 kb on - strandat 2556.858 kb on - strandat 2556.858 kb on - strandat 2557.211 kb on - strand, within RR42_RS32000at 2557.211 kb on - strand, within RR42_RS32000at 2557.214 kb on + strand, within RR42_RS32000at 2557.215 kb on - strand, within RR42_RS32000

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml
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2,554,011 - RR42_RS31985 0.32 -0.7
2,554,011 - RR42_RS31985 0.32 +0.1
2,554,080 - RR42_RS31985 0.41 +1.5
2,554,126 - RR42_RS31985 0.47 +0.6
2,554,126 - RR42_RS31985 0.47 +0.7
2,554,126 - RR42_RS31985 0.47 +0.5
2,554,860 + RR42_RS31990 0.20 +0.2
2,554,941 + RR42_RS31990 0.25 +0.9
2,554,941 + RR42_RS31990 0.25 +0.7
2,554,942 - RR42_RS31990 0.25 -1.3
2,554,942 - RR42_RS31990 0.25 +0.0
2,554,942 - RR42_RS31990 0.25 -0.4
2,554,942 - RR42_RS31990 0.25 -0.5
2,555,370 + RR42_RS31990 0.50 -2.0
2,555,410 + RR42_RS31990 0.52 -0.2
2,555,411 - RR42_RS31990 0.52 -0.7
2,555,808 + RR42_RS31990 0.76 +0.2
2,555,812 - RR42_RS31990 0.76 -0.0
2,555,817 + RR42_RS31990 0.76 +0.6
2,555,818 - RR42_RS31990 0.76 +0.9
2,555,818 - RR42_RS31990 0.76 -0.7
2,555,974 - RR42_RS31990 0.85 -0.9
2,556,043 - RR42_RS31990 0.89 -1.2
2,556,378 + RR42_RS31995 0.11 +0.4
2,556,378 + RR42_RS31995 0.11 +0.8
2,556,545 - RR42_RS31995 0.43 +0.9
2,556,805 + -0.1
2,556,806 - +0.4
2,556,829 + +0.9
2,556,830 - -1.0
2,556,858 - +0.4
2,556,858 - +0.6
2,556,858 - -1.3
2,556,858 - +0.2
2,557,211 - RR42_RS32000 0.18 -0.3
2,557,211 - RR42_RS32000 0.18 -0.8
2,557,214 + RR42_RS32000 0.18 -1.4
2,557,215 - RR42_RS32000 0.18 +0.2

Or see this region's nucleotide sequence