Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31770 and RR42_RS31775 are separated by 88 nucleotides RR42_RS31775 and RR42_RS31780 are separated by 347 nucleotides RR42_RS31780 and RR42_RS31785 are separated by 91 nucleotides
RR42_RS31770: RR42_RS31770 - GNAT family acetyltransferase, at 2,504,338 to 2,504,973
_RS31770
RR42_RS31775: RR42_RS31775 - HxlR family transcriptional regulator, at 2,505,062 to 2,505,565
_RS31775
RR42_RS31780: RR42_RS31780 - MFS transporter, at 2,505,913 to 2,507,235
_RS31780
RR42_RS31785: RR42_RS31785 - Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (from data) , at 2,507,327 to 2,509,876
_RS31785
Position (kb)
2505
2506
2507
2508 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2505.009 kb on + strand at 2505.011 kb on + strand at 2505.011 kb on + strand at 2505.011 kb on + strand at 2505.011 kb on + strand at 2505.012 kb on - strand at 2505.012 kb on - strand at 2505.012 kb on - strand at 2505.012 kb on - strand at 2505.012 kb on - strand at 2505.012 kb on - strand at 2505.050 kb on + strand at 2505.050 kb on + strand at 2505.050 kb on + strand at 2505.050 kb on + strand at 2505.050 kb on + strand at 2505.050 kb on + strand at 2505.051 kb on - strand at 2505.051 kb on - strand at 2505.051 kb on - strand at 2505.051 kb on - strand at 2505.051 kb on - strand at 2505.142 kb on + strand, within RR42_RS31775 at 2505.143 kb on - strand, within RR42_RS31775 at 2505.166 kb on + strand, within RR42_RS31775 at 2505.273 kb on - strand, within RR42_RS31775 at 2505.412 kb on + strand, within RR42_RS31775 at 2505.502 kb on + strand, within RR42_RS31775 at 2505.503 kb on - strand, within RR42_RS31775 at 2505.768 kb on - strand at 2505.768 kb on - strand at 2505.770 kb on + strand at 2505.771 kb on - strand at 2505.901 kb on + strand at 2505.901 kb on + strand at 2505.901 kb on + strand at 2506.231 kb on + strand, within RR42_RS31780 at 2506.231 kb on + strand, within RR42_RS31780 at 2506.232 kb on - strand, within RR42_RS31780 at 2506.232 kb on - strand, within RR42_RS31780 at 2506.232 kb on - strand, within RR42_RS31780 at 2506.232 kb on - strand, within RR42_RS31780 at 2506.298 kb on - strand, within RR42_RS31780 at 2506.504 kb on + strand, within RR42_RS31780 at 2506.504 kb on + strand, within RR42_RS31780 at 2506.505 kb on - strand, within RR42_RS31780 at 2506.505 kb on - strand, within RR42_RS31780 at 2506.515 kb on + strand, within RR42_RS31780 at 2506.515 kb on + strand, within RR42_RS31780 at 2506.515 kb on + strand, within RR42_RS31780 at 2506.516 kb on - strand, within RR42_RS31780 at 2506.516 kb on - strand, within RR42_RS31780 at 2506.625 kb on - strand, within RR42_RS31780 at 2506.651 kb on + strand, within RR42_RS31780 at 2506.652 kb on - strand, within RR42_RS31780 at 2506.652 kb on - strand, within RR42_RS31780 at 2506.702 kb on + strand, within RR42_RS31780 at 2506.702 kb on + strand, within RR42_RS31780 at 2506.702 kb on + strand, within RR42_RS31780 at 2506.702 kb on + strand, within RR42_RS31780 at 2506.703 kb on - strand, within RR42_RS31780 at 2506.705 kb on + strand, within RR42_RS31780 at 2506.705 kb on + strand, within RR42_RS31780 at 2506.705 kb on + strand, within RR42_RS31780 at 2506.706 kb on - strand, within RR42_RS31780 at 2506.706 kb on - strand, within RR42_RS31780 at 2506.706 kb on - strand, within RR42_RS31780 at 2506.706 kb on - strand, within RR42_RS31780 at 2506.979 kb on - strand, within RR42_RS31780 at 2506.979 kb on - strand, within RR42_RS31780 at 2507.444 kb on + strand at 2507.618 kb on + strand, within RR42_RS31785 at 2507.618 kb on + strand, within RR42_RS31785 at 2507.648 kb on + strand, within RR42_RS31785 at 2508.141 kb on - strand, within RR42_RS31785 at 2508.179 kb on + strand, within RR42_RS31785 at 2508.179 kb on + strand, within RR42_RS31785 at 2508.180 kb on - strand, within RR42_RS31785 at 2508.180 kb on - strand, within RR42_RS31785 at 2508.180 kb on - strand, within RR42_RS31785
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,505,009 + +0.5 2,505,011 + +0.6 2,505,011 + -0.4 2,505,011 + -1.2 2,505,011 + +0.3 2,505,012 - +0.0 2,505,012 - -1.1 2,505,012 - +1.1 2,505,012 - -0.3 2,505,012 - -0.4 2,505,012 - +0.3 2,505,050 + +1.9 2,505,050 + +0.3 2,505,050 + +1.1 2,505,050 + +0.6 2,505,050 + +2.7 2,505,050 + +0.6 2,505,051 - -0.8 2,505,051 - -0.8 2,505,051 - +0.0 2,505,051 - +0.0 2,505,051 - -1.3 2,505,142 + RR42_RS31775 0.16 +1.2 2,505,143 - RR42_RS31775 0.16 +0.4 2,505,166 + RR42_RS31775 0.21 +0.6 2,505,273 - RR42_RS31775 0.42 +0.4 2,505,412 + RR42_RS31775 0.69 +0.6 2,505,502 + RR42_RS31775 0.87 -1.4 2,505,503 - RR42_RS31775 0.88 -1.4 2,505,768 - +0.3 2,505,768 - -0.1 2,505,770 + +0.3 2,505,771 - -0.0 2,505,901 + +0.6 2,505,901 + -0.7 2,505,901 + -0.2 2,506,231 + RR42_RS31780 0.24 -0.4 2,506,231 + RR42_RS31780 0.24 +0.4 2,506,232 - RR42_RS31780 0.24 -0.1 2,506,232 - RR42_RS31780 0.24 -1.0 2,506,232 - RR42_RS31780 0.24 +0.7 2,506,232 - RR42_RS31780 0.24 +1.4 2,506,298 - RR42_RS31780 0.29 -0.1 2,506,504 + RR42_RS31780 0.45 -0.1 2,506,504 + RR42_RS31780 0.45 +0.5 2,506,505 - RR42_RS31780 0.45 +0.4 2,506,505 - RR42_RS31780 0.45 -2.8 2,506,515 + RR42_RS31780 0.46 -0.4 2,506,515 + RR42_RS31780 0.46 -0.1 2,506,515 + RR42_RS31780 0.46 +0.3 2,506,516 - RR42_RS31780 0.46 -0.7 2,506,516 - RR42_RS31780 0.46 +0.9 2,506,625 - RR42_RS31780 0.54 +0.3 2,506,651 + RR42_RS31780 0.56 +0.7 2,506,652 - RR42_RS31780 0.56 -0.0 2,506,652 - RR42_RS31780 0.56 -2.0 2,506,702 + RR42_RS31780 0.60 -0.1 2,506,702 + RR42_RS31780 0.60 +0.5 2,506,702 + RR42_RS31780 0.60 -0.9 2,506,702 + RR42_RS31780 0.60 -0.7 2,506,703 - RR42_RS31780 0.60 -0.9 2,506,705 + RR42_RS31780 0.60 +0.9 2,506,705 + RR42_RS31780 0.60 -0.0 2,506,705 + RR42_RS31780 0.60 -0.0 2,506,706 - RR42_RS31780 0.60 -0.5 2,506,706 - RR42_RS31780 0.60 +0.7 2,506,706 - RR42_RS31780 0.60 -0.5 2,506,706 - RR42_RS31780 0.60 +1.7 2,506,979 - RR42_RS31780 0.81 +0.9 2,506,979 - RR42_RS31780 0.81 -1.4 2,507,444 + -1.7 2,507,618 + RR42_RS31785 0.11 +0.7 2,507,618 + RR42_RS31785 0.11 +1.5 2,507,648 + RR42_RS31785 0.13 +0.9 2,508,141 - RR42_RS31785 0.32 -0.4 2,508,179 + RR42_RS31785 0.33 -0.4 2,508,179 + RR42_RS31785 0.33 -1.3 2,508,180 - RR42_RS31785 0.33 -0.4 2,508,180 - RR42_RS31785 0.33 +0.6 2,508,180 - RR42_RS31785 0.33 +0.2
Or see this region's nucleotide sequence