Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31765 and RR42_RS31770 overlap by 4 nucleotides RR42_RS31770 and RR42_RS31775 are separated by 88 nucleotides RR42_RS31775 and RR42_RS31780 are separated by 347 nucleotides
RR42_RS31765: RR42_RS31765 - membrane protein, at 2,503,463 to 2,504,341
_RS31765
RR42_RS31770: RR42_RS31770 - GNAT family acetyltransferase, at 2,504,338 to 2,504,973
_RS31770
RR42_RS31775: RR42_RS31775 - HxlR family transcriptional regulator, at 2,505,062 to 2,505,565
_RS31775
RR42_RS31780: RR42_RS31780 - MFS transporter, at 2,505,913 to 2,507,235
_RS31780
Position (kb)
2505
2506 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2504.126 kb on + strand, within RR42_RS31765 at 2504.246 kb on - strand, within RR42_RS31765 at 2504.414 kb on + strand, within RR42_RS31770 at 2504.414 kb on + strand, within RR42_RS31770 at 2504.414 kb on + strand, within RR42_RS31770 at 2504.415 kb on - strand, within RR42_RS31770 at 2504.415 kb on - strand, within RR42_RS31770 at 2504.555 kb on - strand, within RR42_RS31770 at 2504.648 kb on + strand, within RR42_RS31770 at 2504.649 kb on - strand, within RR42_RS31770 at 2504.649 kb on - strand, within RR42_RS31770 at 2504.649 kb on - strand, within RR42_RS31770 at 2504.740 kb on + strand, within RR42_RS31770 at 2504.780 kb on + strand, within RR42_RS31770 at 2505.009 kb on + strand at 2505.011 kb on + strand at 2505.011 kb on + strand at 2505.011 kb on + strand at 2505.011 kb on + strand at 2505.012 kb on - strand at 2505.012 kb on - strand at 2505.012 kb on - strand at 2505.012 kb on - strand at 2505.012 kb on - strand at 2505.012 kb on - strand at 2505.050 kb on + strand at 2505.050 kb on + strand at 2505.050 kb on + strand at 2505.050 kb on + strand at 2505.050 kb on + strand at 2505.050 kb on + strand at 2505.051 kb on - strand at 2505.051 kb on - strand at 2505.051 kb on - strand at 2505.051 kb on - strand at 2505.051 kb on - strand at 2505.142 kb on + strand, within RR42_RS31775 at 2505.143 kb on - strand, within RR42_RS31775 at 2505.166 kb on + strand, within RR42_RS31775 at 2505.273 kb on - strand, within RR42_RS31775 at 2505.412 kb on + strand, within RR42_RS31775 at 2505.502 kb on + strand, within RR42_RS31775 at 2505.503 kb on - strand, within RR42_RS31775 at 2505.768 kb on - strand at 2505.768 kb on - strand at 2505.770 kb on + strand at 2505.771 kb on - strand at 2505.901 kb on + strand at 2505.901 kb on + strand at 2505.901 kb on + strand at 2506.231 kb on + strand, within RR42_RS31780 at 2506.231 kb on + strand, within RR42_RS31780 at 2506.232 kb on - strand, within RR42_RS31780 at 2506.232 kb on - strand, within RR42_RS31780 at 2506.232 kb on - strand, within RR42_RS31780 at 2506.232 kb on - strand, within RR42_RS31780 at 2506.298 kb on - strand, within RR42_RS31780 at 2506.504 kb on + strand, within RR42_RS31780 at 2506.504 kb on + strand, within RR42_RS31780 at 2506.505 kb on - strand, within RR42_RS31780 at 2506.505 kb on - strand, within RR42_RS31780 at 2506.515 kb on + strand, within RR42_RS31780 at 2506.515 kb on + strand, within RR42_RS31780 at 2506.515 kb on + strand, within RR42_RS31780 at 2506.516 kb on - strand, within RR42_RS31780 at 2506.516 kb on - strand, within RR42_RS31780
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,504,126 + RR42_RS31765 0.75 +0.3 2,504,246 - RR42_RS31765 0.89 -0.7 2,504,414 + RR42_RS31770 0.12 -1.1 2,504,414 + RR42_RS31770 0.12 +0.3 2,504,414 + RR42_RS31770 0.12 +0.0 2,504,415 - RR42_RS31770 0.12 -1.1 2,504,415 - RR42_RS31770 0.12 -1.1 2,504,555 - RR42_RS31770 0.34 -0.5 2,504,648 + RR42_RS31770 0.49 -0.4 2,504,649 - RR42_RS31770 0.49 -0.1 2,504,649 - RR42_RS31770 0.49 +1.1 2,504,649 - RR42_RS31770 0.49 -1.3 2,504,740 + RR42_RS31770 0.63 +0.3 2,504,780 + RR42_RS31770 0.69 +0.7 2,505,009 + +0.5 2,505,011 + +0.6 2,505,011 + -0.4 2,505,011 + -1.2 2,505,011 + +0.3 2,505,012 - +0.0 2,505,012 - -1.1 2,505,012 - +1.1 2,505,012 - -0.3 2,505,012 - -0.4 2,505,012 - +0.3 2,505,050 + +1.9 2,505,050 + +0.3 2,505,050 + +1.1 2,505,050 + +0.6 2,505,050 + +2.7 2,505,050 + +0.6 2,505,051 - -0.8 2,505,051 - -0.8 2,505,051 - +0.0 2,505,051 - +0.0 2,505,051 - -1.3 2,505,142 + RR42_RS31775 0.16 +1.2 2,505,143 - RR42_RS31775 0.16 +0.4 2,505,166 + RR42_RS31775 0.21 +0.6 2,505,273 - RR42_RS31775 0.42 +0.4 2,505,412 + RR42_RS31775 0.69 +0.6 2,505,502 + RR42_RS31775 0.87 -1.4 2,505,503 - RR42_RS31775 0.88 -1.4 2,505,768 - +0.3 2,505,768 - -0.1 2,505,770 + +0.3 2,505,771 - -0.0 2,505,901 + +0.6 2,505,901 + -0.7 2,505,901 + -0.2 2,506,231 + RR42_RS31780 0.24 -0.4 2,506,231 + RR42_RS31780 0.24 +0.4 2,506,232 - RR42_RS31780 0.24 -0.1 2,506,232 - RR42_RS31780 0.24 -1.0 2,506,232 - RR42_RS31780 0.24 +0.7 2,506,232 - RR42_RS31780 0.24 +1.4 2,506,298 - RR42_RS31780 0.29 -0.1 2,506,504 + RR42_RS31780 0.45 -0.1 2,506,504 + RR42_RS31780 0.45 +0.5 2,506,505 - RR42_RS31780 0.45 +0.4 2,506,505 - RR42_RS31780 0.45 -2.8 2,506,515 + RR42_RS31780 0.46 -0.4 2,506,515 + RR42_RS31780 0.46 -0.1 2,506,515 + RR42_RS31780 0.46 +0.3 2,506,516 - RR42_RS31780 0.46 -0.7 2,506,516 - RR42_RS31780 0.46 +0.9
Or see this region's nucleotide sequence