Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS31430

Experiment: R2A with Sisomicin 0.035 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31425 and RR42_RS31430 are separated by 129 nucleotidesRR42_RS31430 and RR42_RS31435 are separated by 28 nucleotidesRR42_RS31435 and RR42_RS31440 are separated by 174 nucleotidesRR42_RS31440 and RR42_RS31445 are separated by 55 nucleotides RR42_RS31425: RR42_RS31425 - chloroperoxidase, at 2,432,437 to 2,433,123 _RS31425 RR42_RS31430: RR42_RS31430 - amidohydrolase, at 2,433,253 to 2,435,124 _RS31430 RR42_RS31435: RR42_RS31435 - hypothetical protein, at 2,435,153 to 2,435,587 _RS31435 RR42_RS31440: RR42_RS31440 - ABC transporter substrate-binding protein, at 2,435,762 to 2,436,055 _RS31440 RR42_RS31445: RR42_RS31445 - porin, at 2,436,111 to 2,437,208 _RS31445 Position (kb) 2433 2434 2435 2436Strain fitness (log2 ratio) -2 -1 0 1 2at 2432.266 kb on + strandat 2432.267 kb on - strandat 2432.270 kb on - strandat 2432.270 kb on - strandat 2432.306 kb on - strandat 2432.354 kb on + strandat 2432.409 kb on + strandat 2432.592 kb on + strand, within RR42_RS31425at 2433.043 kb on + strand, within RR42_RS31425at 2433.049 kb on + strand, within RR42_RS31425at 2433.121 kb on + strandat 2433.130 kb on - strandat 2433.144 kb on + strandat 2433.176 kb on - strandat 2433.245 kb on + strandat 2433.246 kb on - strandat 2433.373 kb on - strandat 2433.373 kb on - strandat 2433.494 kb on - strand, within RR42_RS31430at 2433.701 kb on - strand, within RR42_RS31430at 2433.747 kb on + strand, within RR42_RS31430at 2433.848 kb on - strand, within RR42_RS31430at 2433.989 kb on - strand, within RR42_RS31430at 2434.043 kb on - strand, within RR42_RS31430at 2434.165 kb on + strand, within RR42_RS31430at 2434.294 kb on + strand, within RR42_RS31430at 2434.294 kb on + strand, within RR42_RS31430at 2434.294 kb on + strand, within RR42_RS31430at 2434.295 kb on - strand, within RR42_RS31430at 2434.463 kb on - strand, within RR42_RS31430at 2434.475 kb on - strand, within RR42_RS31430at 2434.528 kb on + strand, within RR42_RS31430at 2434.588 kb on + strand, within RR42_RS31430at 2434.588 kb on + strand, within RR42_RS31430at 2434.588 kb on + strand, within RR42_RS31430at 2434.610 kb on - strand, within RR42_RS31430at 2434.610 kb on - strand, within RR42_RS31430at 2434.610 kb on - strand, within RR42_RS31430at 2434.793 kb on - strand, within RR42_RS31430at 2434.795 kb on + strand, within RR42_RS31430at 2434.864 kb on + strand, within RR42_RS31430at 2435.194 kb on + strandat 2435.253 kb on - strand, within RR42_RS31435at 2435.664 kb on - strandat 2435.761 kb on + strandat 2435.761 kb on + strandat 2435.761 kb on + strandat 2435.762 kb on - strandat 2435.762 kb on - strandat 2435.771 kb on + strandat 2435.771 kb on + strandat 2435.772 kb on - strandat 2435.813 kb on + strand, within RR42_RS31440at 2435.813 kb on + strand, within RR42_RS31440at 2435.813 kb on + strand, within RR42_RS31440at 2435.814 kb on - strand, within RR42_RS31440at 2435.814 kb on - strand, within RR42_RS31440at 2435.814 kb on - strand, within RR42_RS31440at 2435.814 kb on - strand, within RR42_RS31440at 2435.814 kb on - strand, within RR42_RS31440at 2435.814 kb on - strand, within RR42_RS31440at 2435.814 kb on - strand, within RR42_RS31440at 2435.814 kb on - strand, within RR42_RS31440at 2436.035 kb on - strandat 2436.092 kb on + strandat 2436.093 kb on - strandat 2436.093 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml
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2,432,266 + +0.8
2,432,267 - +0.1
2,432,270 - -1.3
2,432,270 - +1.1
2,432,306 - -0.4
2,432,354 + -0.6
2,432,409 + -0.4
2,432,592 + RR42_RS31425 0.23 -0.5
2,433,043 + RR42_RS31425 0.88 -0.6
2,433,049 + RR42_RS31425 0.89 -0.1
2,433,121 + -0.1
2,433,130 - -0.4
2,433,144 + -1.0
2,433,176 - +1.9
2,433,245 + -0.8
2,433,246 - -0.1
2,433,373 - -0.7
2,433,373 - -0.2
2,433,494 - RR42_RS31430 0.13 +0.9
2,433,701 - RR42_RS31430 0.24 -0.6
2,433,747 + RR42_RS31430 0.26 +0.3
2,433,848 - RR42_RS31430 0.32 -0.3
2,433,989 - RR42_RS31430 0.39 +0.2
2,434,043 - RR42_RS31430 0.42 +1.2
2,434,165 + RR42_RS31430 0.49 -0.1
2,434,294 + RR42_RS31430 0.56 +0.5
2,434,294 + RR42_RS31430 0.56 +0.4
2,434,294 + RR42_RS31430 0.56 -1.4
2,434,295 - RR42_RS31430 0.56 +0.5
2,434,463 - RR42_RS31430 0.65 -0.9
2,434,475 - RR42_RS31430 0.65 -2.4
2,434,528 + RR42_RS31430 0.68 -0.7
2,434,588 + RR42_RS31430 0.71 +1.3
2,434,588 + RR42_RS31430 0.71 -0.4
2,434,588 + RR42_RS31430 0.71 -0.0
2,434,610 - RR42_RS31430 0.72 -0.3
2,434,610 - RR42_RS31430 0.72 -0.0
2,434,610 - RR42_RS31430 0.72 +0.1
2,434,793 - RR42_RS31430 0.82 -1.0
2,434,795 + RR42_RS31430 0.82 +0.0
2,434,864 + RR42_RS31430 0.86 -0.4
2,435,194 + -0.5
2,435,253 - RR42_RS31435 0.23 -1.6
2,435,664 - +0.5
2,435,761 + -0.1
2,435,761 + -1.4
2,435,761 + +0.9
2,435,762 - -0.3
2,435,762 - +0.4
2,435,771 + +0.2
2,435,771 + -0.1
2,435,772 - -0.4
2,435,813 + RR42_RS31440 0.17 -0.4
2,435,813 + RR42_RS31440 0.17 -0.6
2,435,813 + RR42_RS31440 0.17 +0.3
2,435,814 - RR42_RS31440 0.18 +0.5
2,435,814 - RR42_RS31440 0.18 +0.6
2,435,814 - RR42_RS31440 0.18 +0.3
2,435,814 - RR42_RS31440 0.18 -0.6
2,435,814 - RR42_RS31440 0.18 -1.9
2,435,814 - RR42_RS31440 0.18 +0.3
2,435,814 - RR42_RS31440 0.18 +0.7
2,435,814 - RR42_RS31440 0.18 -0.4
2,436,035 - -0.1
2,436,092 + -0.5
2,436,093 - +0.1
2,436,093 - +1.6

Or see this region's nucleotide sequence