Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31285 and RR42_RS31290 overlap by 96 nucleotides RR42_RS31290 and RR42_RS31295 are separated by 265 nucleotides RR42_RS31295 and RR42_RS31300 are separated by 257 nucleotides
RR42_RS31285: RR42_RS31285 - XRE family transcriptional regulator, at 2,393,583 to 2,393,816
_RS31285
RR42_RS31290: RR42_RS31290 - XRE family transcriptional regulator, at 2,393,721 to 2,393,975
_RS31290
RR42_RS31295: RR42_RS31295 - XRE family transcriptional regulator, at 2,394,241 to 2,394,849
_RS31295
RR42_RS31300: RR42_RS31300 - TetR family transcriptional regulator, at 2,395,107 to 2,396,009
_RS31300
Position (kb)
2394
2395 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2393.253 kb on + strand at 2393.253 kb on + strand at 2393.253 kb on + strand at 2393.253 kb on + strand at 2393.254 kb on - strand at 2393.254 kb on - strand at 2393.261 kb on + strand at 2393.261 kb on + strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.656 kb on - strand, within RR42_RS31285 at 2393.730 kb on - strand at 2393.730 kb on - strand at 2393.852 kb on - strand, within RR42_RS31290 at 2393.852 kb on - strand, within RR42_RS31290 at 2393.966 kb on + strand at 2393.967 kb on - strand at 2394.028 kb on + strand at 2394.028 kb on + strand at 2394.029 kb on - strand at 2394.049 kb on + strand at 2394.049 kb on + strand at 2394.050 kb on - strand at 2394.050 kb on - strand at 2394.050 kb on - strand at 2394.053 kb on + strand at 2394.054 kb on - strand at 2394.109 kb on + strand at 2394.109 kb on + strand at 2394.109 kb on + strand at 2394.110 kb on - strand at 2394.110 kb on - strand at 2394.110 kb on - strand at 2394.120 kb on + strand at 2394.121 kb on - strand at 2394.129 kb on + strand at 2394.129 kb on + strand at 2394.129 kb on + strand at 2394.141 kb on - strand at 2394.141 kb on - strand at 2394.178 kb on + strand at 2394.178 kb on + strand at 2394.258 kb on + strand at 2394.326 kb on + strand, within RR42_RS31295 at 2394.370 kb on + strand, within RR42_RS31295 at 2394.508 kb on + strand, within RR42_RS31295 at 2394.508 kb on + strand, within RR42_RS31295 at 2394.508 kb on + strand, within RR42_RS31295 at 2394.508 kb on + strand, within RR42_RS31295 at 2394.508 kb on + strand, within RR42_RS31295 at 2394.574 kb on + strand, within RR42_RS31295 at 2394.751 kb on + strand, within RR42_RS31295 at 2394.751 kb on + strand, within RR42_RS31295 at 2394.752 kb on - strand, within RR42_RS31295 at 2394.759 kb on - strand, within RR42_RS31295 at 2394.759 kb on - strand, within RR42_RS31295 at 2394.759 kb on - strand, within RR42_RS31295 at 2394.759 kb on - strand, within RR42_RS31295 at 2394.759 kb on - strand, within RR42_RS31295 at 2394.848 kb on - strand at 2394.848 kb on - strand at 2394.977 kb on + strand at 2395.054 kb on - strand at 2395.103 kb on - strand at 2395.537 kb on + strand, within RR42_RS31300 at 2395.537 kb on + strand, within RR42_RS31300 at 2395.538 kb on - strand at 2395.606 kb on + strand, within RR42_RS31300 at 2395.682 kb on - strand, within RR42_RS31300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,393,253 + +0.2 2,393,253 + -0.6 2,393,253 + +0.3 2,393,253 + -0.1 2,393,254 - -0.4 2,393,254 - -2.4 2,393,261 + +0.2 2,393,261 + -0.4 2,393,262 - +0.0 2,393,262 - +0.6 2,393,262 - +0.2 2,393,262 - +0.1 2,393,262 - +0.0 2,393,262 - -0.5 2,393,262 - +0.9 2,393,262 - -1.2 2,393,656 - RR42_RS31285 0.31 +1.1 2,393,730 - -0.4 2,393,730 - +0.7 2,393,852 - RR42_RS31290 0.51 +0.9 2,393,852 - RR42_RS31290 0.51 +0.7 2,393,966 + +3.3 2,393,967 - +0.9 2,394,028 + +0.9 2,394,028 + +0.4 2,394,029 - +1.2 2,394,049 + +1.6 2,394,049 + +2.5 2,394,050 - -0.0 2,394,050 - -0.5 2,394,050 - -0.3 2,394,053 + +1.7 2,394,054 - +0.5 2,394,109 + +1.5 2,394,109 + +1.5 2,394,109 + +1.6 2,394,110 - +0.5 2,394,110 - -1.4 2,394,110 - -1.7 2,394,120 + +2.3 2,394,121 - +0.8 2,394,129 + +1.6 2,394,129 + +1.1 2,394,129 + +3.3 2,394,141 - -0.1 2,394,141 - +0.2 2,394,178 + -2.3 2,394,178 + -1.2 2,394,258 + -0.1 2,394,326 + RR42_RS31295 0.14 +1.3 2,394,370 + RR42_RS31295 0.21 -0.2 2,394,508 + RR42_RS31295 0.44 +0.3 2,394,508 + RR42_RS31295 0.44 +0.7 2,394,508 + RR42_RS31295 0.44 +0.3 2,394,508 + RR42_RS31295 0.44 -0.6 2,394,508 + RR42_RS31295 0.44 -0.6 2,394,574 + RR42_RS31295 0.55 +0.1 2,394,751 + RR42_RS31295 0.84 +1.0 2,394,751 + RR42_RS31295 0.84 +1.3 2,394,752 - RR42_RS31295 0.84 -0.1 2,394,759 - RR42_RS31295 0.85 -0.9 2,394,759 - RR42_RS31295 0.85 -0.2 2,394,759 - RR42_RS31295 0.85 +0.3 2,394,759 - RR42_RS31295 0.85 -0.2 2,394,759 - RR42_RS31295 0.85 -1.1 2,394,848 - -0.4 2,394,848 - -0.7 2,394,977 + -0.6 2,395,054 - -0.6 2,395,103 - +0.6 2,395,537 + RR42_RS31300 0.48 +1.3 2,395,537 + RR42_RS31300 0.48 +1.3 2,395,538 - -1.1 2,395,606 + RR42_RS31300 0.55 -1.0 2,395,682 - RR42_RS31300 0.64 -0.1
Or see this region's nucleotide sequence