Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31005 and RR42_RS31010 are separated by 79 nucleotides RR42_RS31010 and RR42_RS31015 are separated by 27 nucleotides RR42_RS31015 and RR42_RS31020 are separated by 6 nucleotides
RR42_RS31005: RR42_RS31005 - homospermidine synthase, at 2,336,915 to 2,338,240
_RS31005
RR42_RS31010: RR42_RS31010 - phosphatidate cytidylyltransferase, at 2,338,320 to 2,339,261
_RS31010
RR42_RS31015: RR42_RS31015 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 2,339,289 to 2,339,915
_RS31015
RR42_RS31020: RR42_RS31020 - hypothetical protein, at 2,339,922 to 2,340,362
_RS31020
Position (kb)
2338
2339
2340 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2337.360 kb on + strand, within RR42_RS31005 at 2337.399 kb on + strand, within RR42_RS31005 at 2337.592 kb on + strand, within RR42_RS31005 at 2337.592 kb on + strand, within RR42_RS31005 at 2337.593 kb on - strand, within RR42_RS31005 at 2337.867 kb on + strand, within RR42_RS31005 at 2337.867 kb on + strand, within RR42_RS31005 at 2337.868 kb on - strand, within RR42_RS31005 at 2337.868 kb on - strand, within RR42_RS31005 at 2337.904 kb on - strand, within RR42_RS31005 at 2338.138 kb on + strand at 2338.138 kb on + strand at 2338.216 kb on + strand at 2338.235 kb on - strand at 2338.235 kb on - strand at 2338.648 kb on - strand, within RR42_RS31010 at 2338.666 kb on + strand, within RR42_RS31010 at 2338.666 kb on + strand, within RR42_RS31010 at 2338.666 kb on + strand, within RR42_RS31010 at 2338.666 kb on + strand, within RR42_RS31010 at 2338.667 kb on - strand, within RR42_RS31010 at 2338.667 kb on - strand, within RR42_RS31010 at 2338.667 kb on - strand, within RR42_RS31010 at 2338.927 kb on + strand, within RR42_RS31010 at 2338.939 kb on + strand, within RR42_RS31010 at 2338.939 kb on + strand, within RR42_RS31010 at 2338.940 kb on - strand, within RR42_RS31010 at 2338.940 kb on - strand, within RR42_RS31010 at 2338.940 kb on - strand, within RR42_RS31010 at 2339.047 kb on + strand, within RR42_RS31010 at 2339.048 kb on - strand, within RR42_RS31010 at 2339.048 kb on - strand, within RR42_RS31010 at 2339.218 kb on - strand at 2339.290 kb on + strand at 2339.503 kb on + strand, within RR42_RS31015 at 2339.503 kb on + strand, within RR42_RS31015 at 2339.504 kb on - strand, within RR42_RS31015 at 2339.504 kb on - strand, within RR42_RS31015 at 2339.504 kb on - strand, within RR42_RS31015 at 2339.543 kb on + strand, within RR42_RS31015 at 2339.543 kb on + strand, within RR42_RS31015 at 2339.543 kb on + strand, within RR42_RS31015 at 2339.544 kb on - strand, within RR42_RS31015 at 2339.664 kb on + strand, within RR42_RS31015 at 2339.664 kb on + strand, within RR42_RS31015 at 2339.664 kb on + strand, within RR42_RS31015 at 2339.664 kb on + strand, within RR42_RS31015 at 2339.664 kb on + strand, within RR42_RS31015 at 2339.665 kb on - strand, within RR42_RS31015 at 2339.806 kb on + strand, within RR42_RS31015 at 2339.806 kb on + strand, within RR42_RS31015 at 2339.810 kb on + strand, within RR42_RS31015 at 2339.810 kb on + strand, within RR42_RS31015 at 2339.811 kb on - strand, within RR42_RS31015 at 2339.811 kb on - strand, within RR42_RS31015 at 2339.924 kb on - strand at 2339.938 kb on - strand at 2339.938 kb on - strand at 2339.938 kb on - strand at 2339.938 kb on - strand at 2339.938 kb on - strand at 2340.220 kb on + strand, within RR42_RS31020 at 2340.220 kb on + strand, within RR42_RS31020 at 2340.220 kb on + strand, within RR42_RS31020 at 2340.221 kb on - strand, within RR42_RS31020 at 2340.221 kb on - strand, within RR42_RS31020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,337,360 + RR42_RS31005 0.34 -0.7 2,337,399 + RR42_RS31005 0.37 -0.1 2,337,592 + RR42_RS31005 0.51 -1.4 2,337,592 + RR42_RS31005 0.51 +0.5 2,337,593 - RR42_RS31005 0.51 -0.1 2,337,867 + RR42_RS31005 0.72 -1.4 2,337,867 + RR42_RS31005 0.72 +0.9 2,337,868 - RR42_RS31005 0.72 +0.4 2,337,868 - RR42_RS31005 0.72 +0.9 2,337,904 - RR42_RS31005 0.75 -0.6 2,338,138 + +1.5 2,338,138 + -0.1 2,338,216 + -0.7 2,338,235 - +0.1 2,338,235 - -0.1 2,338,648 - RR42_RS31010 0.35 -2.8 2,338,666 + RR42_RS31010 0.37 +0.9 2,338,666 + RR42_RS31010 0.37 -0.7 2,338,666 + RR42_RS31010 0.37 +0.1 2,338,666 + RR42_RS31010 0.37 +0.7 2,338,667 - RR42_RS31010 0.37 -0.3 2,338,667 - RR42_RS31010 0.37 -1.1 2,338,667 - RR42_RS31010 0.37 -1.4 2,338,927 + RR42_RS31010 0.64 +0.1 2,338,939 + RR42_RS31010 0.66 -0.1 2,338,939 + RR42_RS31010 0.66 +0.5 2,338,940 - RR42_RS31010 0.66 -0.9 2,338,940 - RR42_RS31010 0.66 -0.8 2,338,940 - RR42_RS31010 0.66 -0.3 2,339,047 + RR42_RS31010 0.77 +0.2 2,339,048 - RR42_RS31010 0.77 -0.3 2,339,048 - RR42_RS31010 0.77 -0.7 2,339,218 - +1.3 2,339,290 + +0.9 2,339,503 + RR42_RS31015 0.34 +0.1 2,339,503 + RR42_RS31015 0.34 -1.4 2,339,504 - RR42_RS31015 0.34 -0.7 2,339,504 - RR42_RS31015 0.34 -0.6 2,339,504 - RR42_RS31015 0.34 +0.6 2,339,543 + RR42_RS31015 0.41 +0.2 2,339,543 + RR42_RS31015 0.41 -0.1 2,339,543 + RR42_RS31015 0.41 +0.6 2,339,544 - RR42_RS31015 0.41 +0.9 2,339,664 + RR42_RS31015 0.60 -0.3 2,339,664 + RR42_RS31015 0.60 +1.3 2,339,664 + RR42_RS31015 0.60 +0.9 2,339,664 + RR42_RS31015 0.60 -0.5 2,339,664 + RR42_RS31015 0.60 -0.4 2,339,665 - RR42_RS31015 0.60 -2.4 2,339,806 + RR42_RS31015 0.82 -1.0 2,339,806 + RR42_RS31015 0.82 -1.4 2,339,810 + RR42_RS31015 0.83 +0.9 2,339,810 + RR42_RS31015 0.83 -0.2 2,339,811 - RR42_RS31015 0.83 -1.0 2,339,811 - RR42_RS31015 0.83 -2.1 2,339,924 - -0.1 2,339,938 - -0.3 2,339,938 - -0.3 2,339,938 - -0.4 2,339,938 - +0.5 2,339,938 - +0.2 2,340,220 + RR42_RS31020 0.68 -0.0 2,340,220 + RR42_RS31020 0.68 +0.3 2,340,220 + RR42_RS31020 0.68 +0.4 2,340,221 - RR42_RS31020 0.68 -1.1 2,340,221 - RR42_RS31020 0.68 +0.3
Or see this region's nucleotide sequence