Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS30220

Experiment: R2A with Sisomicin 0.035 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Sisomicin 0.035 mg/ml
2,154,622	-		RR42_RS30215	0.38	+1.9
2,154,630	+		RR42_RS30215	0.38	+1.4
2,154,631	-		RR42_RS30215	0.39	+0.7
2,154,664	-		RR42_RS30215	0.41	+0.2
2,154,670	+		RR42_RS30215	0.42	+0.1
2,154,671	-		RR42_RS30215	0.42	+0.9
2,154,671	-		RR42_RS30215	0.42	-0.3
2,154,671	-		RR42_RS30215	0.42	+0.4
2,154,671	-		RR42_RS30215	0.42	+0.1
2,154,671	-		RR42_RS30215	0.42	+0.7
2,154,671	-		RR42_RS30215	0.42	-2.4
2,154,672	+		RR42_RS30215	0.42	+1.5
2,154,672	+		RR42_RS30215	0.42	+0.7
2,154,672	+		RR42_RS30215	0.42	-0.3
2,154,673	-		RR42_RS30215	0.42	-0.9
2,154,940	+		RR42_RS30215	0.62	+0.3
2,154,940	+		RR42_RS30215	0.62	-0.2
2,154,940	+		RR42_RS30215	0.62	+0.4
2,155,227	-		RR42_RS30215	0.84	+1.0
2,155,267	+		RR42_RS30215	0.88	-0.1
2,155,267	+		RR42_RS30215	0.88	-0.5
2,155,267	+		RR42_RS30215	0.88	+0.6
2,155,269	+		RR42_RS30215	0.88	-2.4
2,155,327	+				-0.7
2,155,328	-				-1.2
2,155,360	+				+0.7
2,155,361	-				-0.4
2,155,361	-				-0.5
2,155,427	-				+0.1
2,155,435	+				+1.5
2,155,436	-				-0.8
2,155,436	-				+0.2
2,155,436	-				+0.2
2,155,622	-				+0.2
2,155,663	+				-0.1
2,155,719	+		RR42_RS30220	0.13	+0.9
2,155,720	-		RR42_RS30220	0.13	-1.1
2,156,080	+		RR42_RS30220	0.44	-0.1
2,156,080	+		RR42_RS30220	0.44	+0.1
2,156,081	-		RR42_RS30220	0.44	+0.0
2,156,081	-		RR42_RS30220	0.44	-2.1
2,156,081	-		RR42_RS30220	0.44	+0.9
2,156,081	-		RR42_RS30220	0.44	-0.1
2,156,081	-		RR42_RS30220	0.44	-0.1
2,156,081	-		RR42_RS30220	0.44	-0.8
2,156,081	-		RR42_RS30220	0.44	-0.4
2,156,081	-		RR42_RS30220	0.44	-0.0
2,156,182	+		RR42_RS30220	0.53	+0.0
2,156,183	-		RR42_RS30220	0.53	+0.0
2,156,288	-		RR42_RS30220	0.62	-1.0
2,156,289	+		RR42_RS30220	0.62	-1.4
2,156,311	+		RR42_RS30220	0.64	+0.2
2,156,395	+		RR42_RS30220	0.71	-1.8
2,156,531	+		RR42_RS30220	0.83	-0.4
2,156,532	-		RR42_RS30220	0.83	+1.2
2,156,532	-		RR42_RS30220	0.83	-0.6
2,156,549	-		RR42_RS30220	0.85	-0.7
2,156,620	+				+0.5
2,156,621	-				-1.4
2,156,621	-				-0.5
2,156,870	+				-0.4
2,156,870	+				-0.5
2,156,870	+				+0.6
2,156,870	+				-0.2
2,156,985	+		RR42_RS30225	0.12	+0.3
2,156,985	+		RR42_RS30225	0.12	-1.0
2,156,986	-		RR42_RS30225	0.13	+1.6
2,156,987	+		RR42_RS30225	0.13	+1.9
2,156,988	-		RR42_RS30225	0.13	+0.2
2,157,117	+		RR42_RS30225	0.23	-0.5
2,157,117	+		RR42_RS30225	0.23	-1.4
2,157,118	-		RR42_RS30225	0.23	-1.4
2,157,296	+		RR42_RS30225	0.37	+0.6
2,157,592	+		RR42_RS30225	0.60	+0.9
2,157,640	-		RR42_RS30225	0.64	-1.8