Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30165 and RR42_RS30170 are separated by 123 nucleotides RR42_RS30170 and RR42_RS30175 are separated by 154 nucleotides
RR42_RS30165: RR42_RS30165 - membrane protein, at 2,141,524 to 2,142,720
_RS30165
RR42_RS30170: RR42_RS30170 - aldolase, at 2,142,844 to 2,143,608
_RS30170
RR42_RS30175: RR42_RS30175 - beta-glucosidase, at 2,143,763 to 2,145,889
_RS30175
Position (kb)
2142
2143
2144 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2141.924 kb on + strand, within RR42_RS30165 at 2141.924 kb on + strand, within RR42_RS30165 at 2141.925 kb on - strand, within RR42_RS30165 at 2141.925 kb on - strand, within RR42_RS30165 at 2142.029 kb on + strand, within RR42_RS30165 at 2142.029 kb on + strand, within RR42_RS30165 at 2142.030 kb on - strand, within RR42_RS30165 at 2142.054 kb on + strand, within RR42_RS30165 at 2142.055 kb on - strand, within RR42_RS30165 at 2142.143 kb on + strand, within RR42_RS30165 at 2142.144 kb on - strand, within RR42_RS30165 at 2142.182 kb on + strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.512 kb on - strand, within RR42_RS30165 at 2142.626 kb on + strand at 2142.626 kb on + strand at 2142.626 kb on + strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.705 kb on - strand at 2142.705 kb on - strand at 2142.705 kb on - strand at 2142.709 kb on - strand at 2142.709 kb on - strand at 2142.709 kb on - strand at 2142.730 kb on + strand at 2142.730 kb on + strand at 2142.733 kb on + strand at 2142.734 kb on - strand at 2142.734 kb on - strand at 2142.845 kb on + strand at 2142.846 kb on - strand at 2142.846 kb on - strand at 2143.034 kb on + strand, within RR42_RS30170 at 2143.034 kb on + strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.266 kb on + strand, within RR42_RS30170 at 2143.272 kb on + strand, within RR42_RS30170 at 2143.272 kb on + strand, within RR42_RS30170 at 2143.600 kb on - strand at 2143.630 kb on + strand at 2143.731 kb on + strand at 2143.793 kb on + strand at 2143.793 kb on + strand at 2143.793 kb on + strand at 2143.794 kb on - strand at 2143.915 kb on + strand at 2144.045 kb on - strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.119 kb on - strand, within RR42_RS30175 at 2144.202 kb on + strand, within RR42_RS30175 at 2144.323 kb on + strand, within RR42_RS30175 at 2144.539 kb on - strand, within RR42_RS30175
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,141,924 + RR42_RS30165 0.33 -0.3 2,141,924 + RR42_RS30165 0.33 +1.5 2,141,925 - RR42_RS30165 0.34 +0.9 2,141,925 - RR42_RS30165 0.34 +1.1 2,142,029 + RR42_RS30165 0.42 +0.3 2,142,029 + RR42_RS30165 0.42 +0.4 2,142,030 - RR42_RS30165 0.42 -0.4 2,142,054 + RR42_RS30165 0.44 +0.7 2,142,055 - RR42_RS30165 0.44 +0.1 2,142,143 + RR42_RS30165 0.52 -0.0 2,142,144 - RR42_RS30165 0.52 +0.4 2,142,182 + RR42_RS30165 0.55 -0.1 2,142,294 - RR42_RS30165 0.64 +0.7 2,142,294 - RR42_RS30165 0.64 -0.5 2,142,294 - RR42_RS30165 0.64 +0.2 2,142,294 - RR42_RS30165 0.64 +0.1 2,142,512 - RR42_RS30165 0.83 +1.4 2,142,626 + +0.1 2,142,626 + +0.0 2,142,626 + -1.1 2,142,627 - -1.5 2,142,627 - +0.6 2,142,627 - +1.0 2,142,627 - +0.6 2,142,705 - +0.9 2,142,705 - -0.2 2,142,705 - -0.2 2,142,709 - -0.5 2,142,709 - -1.7 2,142,709 - -1.5 2,142,730 + +1.1 2,142,730 + +1.5 2,142,733 + +0.0 2,142,734 - +1.9 2,142,734 - -0.1 2,142,845 + +0.7 2,142,846 - -0.7 2,142,846 - -1.3 2,143,034 + RR42_RS30170 0.25 -1.7 2,143,034 + RR42_RS30170 0.25 +0.6 2,143,035 - RR42_RS30170 0.25 -0.3 2,143,035 - RR42_RS30170 0.25 +0.5 2,143,035 - RR42_RS30170 0.25 +0.3 2,143,266 + RR42_RS30170 0.55 +0.1 2,143,272 + RR42_RS30170 0.56 -1.7 2,143,272 + RR42_RS30170 0.56 -0.8 2,143,600 - +0.9 2,143,630 + -0.6 2,143,731 + +0.9 2,143,793 + +0.2 2,143,793 + +0.7 2,143,793 + +0.4 2,143,794 - -0.2 2,143,915 + +1.6 2,144,045 - RR42_RS30175 0.13 -0.0 2,144,118 + RR42_RS30175 0.17 -0.3 2,144,118 + RR42_RS30175 0.17 +0.2 2,144,118 + RR42_RS30175 0.17 -1.9 2,144,118 + RR42_RS30175 0.17 -0.9 2,144,119 - RR42_RS30175 0.17 -0.9 2,144,202 + RR42_RS30175 0.21 -0.1 2,144,323 + RR42_RS30175 0.26 +0.4 2,144,539 - RR42_RS30175 0.36 -0.6
Or see this region's nucleotide sequence