Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28940 and RR42_RS28945 are separated by 73 nucleotides RR42_RS28945 and RR42_RS28950 are separated by 233 nucleotides
RR42_RS28940: RR42_RS28940 - Bcr/CflA family drug resistance efflux transporter, at 1,851,054 to 1,852,274
_RS28940
RR42_RS28945: RR42_RS28945 - mechanosensitive ion channel protein MscS, at 1,852,348 to 1,853,178
_RS28945
RR42_RS28950: RR42_RS28950 - ammonium transporter, at 1,853,412 to 1,854,614
_RS28950
Position (kb)
1852
1853
1854 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1851.618 kb on + strand, within RR42_RS28940 at 1851.618 kb on + strand, within RR42_RS28940 at 1851.618 kb on + strand, within RR42_RS28940 at 1851.618 kb on + strand, within RR42_RS28940 at 1851.619 kb on - strand, within RR42_RS28940 at 1851.619 kb on - strand, within RR42_RS28940 at 1851.620 kb on + strand, within RR42_RS28940 at 1851.620 kb on + strand, within RR42_RS28940 at 1851.621 kb on - strand, within RR42_RS28940 at 1851.685 kb on - strand, within RR42_RS28940 at 1851.720 kb on + strand, within RR42_RS28940 at 1851.762 kb on + strand, within RR42_RS28940 at 1851.762 kb on + strand, within RR42_RS28940 at 1852.020 kb on - strand, within RR42_RS28940 at 1852.316 kb on + strand at 1852.349 kb on + strand at 1852.451 kb on + strand, within RR42_RS28945 at 1852.451 kb on + strand, within RR42_RS28945 at 1852.451 kb on + strand, within RR42_RS28945 at 1852.451 kb on + strand, within RR42_RS28945 at 1852.451 kb on + strand, within RR42_RS28945 at 1852.452 kb on - strand, within RR42_RS28945 at 1852.452 kb on - strand, within RR42_RS28945 at 1852.452 kb on - strand, within RR42_RS28945 at 1852.452 kb on - strand, within RR42_RS28945 at 1852.452 kb on - strand, within RR42_RS28945 at 1852.452 kb on - strand, within RR42_RS28945 at 1852.474 kb on - strand, within RR42_RS28945 at 1852.481 kb on + strand, within RR42_RS28945 at 1852.482 kb on - strand, within RR42_RS28945 at 1852.490 kb on - strand, within RR42_RS28945 at 1852.636 kb on + strand, within RR42_RS28945 at 1852.637 kb on - strand, within RR42_RS28945 at 1852.652 kb on + strand, within RR42_RS28945 at 1852.937 kb on + strand, within RR42_RS28945 at 1852.938 kb on - strand, within RR42_RS28945 at 1852.946 kb on - strand at 1852.965 kb on + strand, within RR42_RS28945 at 1852.998 kb on - strand, within RR42_RS28945 at 1853.132 kb on + strand at 1853.132 kb on + strand at 1853.132 kb on + strand at 1853.132 kb on + strand at 1853.132 kb on + strand at 1853.132 kb on + strand at 1853.132 kb on + strand at 1853.133 kb on - strand at 1853.133 kb on - strand at 1853.133 kb on - strand at 1853.133 kb on - strand at 1853.133 kb on - strand at 1853.133 kb on - strand at 1853.133 kb on - strand at 1853.133 kb on - strand at 1853.133 kb on - strand at 1853.133 kb on - strand at 1853.153 kb on + strand at 1853.154 kb on - strand at 1853.169 kb on + strand at 1853.169 kb on + strand at 1853.169 kb on + strand at 1853.169 kb on + strand at 1853.169 kb on + strand at 1853.169 kb on + strand at 1853.169 kb on + strand at 1853.169 kb on + strand at 1853.170 kb on - strand at 1853.170 kb on - strand at 1853.170 kb on - strand at 1853.170 kb on - strand at 1853.207 kb on + strand at 1853.207 kb on + strand at 1853.207 kb on + strand at 1853.207 kb on + strand at 1853.208 kb on - strand at 1853.208 kb on - strand at 1853.280 kb on + strand at 1853.280 kb on + strand at 1853.414 kb on - strand at 1853.476 kb on + strand at 1853.476 kb on + strand at 1853.477 kb on - strand at 1853.547 kb on + strand, within RR42_RS28950 at 1853.738 kb on - strand, within RR42_RS28950 at 1853.842 kb on + strand, within RR42_RS28950 at 1853.842 kb on + strand, within RR42_RS28950 at 1853.843 kb on - strand, within RR42_RS28950 at 1853.843 kb on - strand, within RR42_RS28950
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,851,618 + RR42_RS28940 0.46 +0.9 1,851,618 + RR42_RS28940 0.46 +0.9 1,851,618 + RR42_RS28940 0.46 -0.3 1,851,618 + RR42_RS28940 0.46 +0.3 1,851,619 - RR42_RS28940 0.46 +0.8 1,851,619 - RR42_RS28940 0.46 -0.3 1,851,620 + RR42_RS28940 0.46 -1.1 1,851,620 + RR42_RS28940 0.46 +0.6 1,851,621 - RR42_RS28940 0.46 +1.0 1,851,685 - RR42_RS28940 0.52 +1.9 1,851,720 + RR42_RS28940 0.55 +0.5 1,851,762 + RR42_RS28940 0.58 +0.2 1,851,762 + RR42_RS28940 0.58 -0.7 1,852,020 - RR42_RS28940 0.79 -1.1 1,852,316 + -0.2 1,852,349 + +0.1 1,852,451 + RR42_RS28945 0.12 -0.9 1,852,451 + RR42_RS28945 0.12 +0.5 1,852,451 + RR42_RS28945 0.12 -1.2 1,852,451 + RR42_RS28945 0.12 +0.5 1,852,451 + RR42_RS28945 0.12 -0.7 1,852,452 - RR42_RS28945 0.13 +0.4 1,852,452 - RR42_RS28945 0.13 -0.1 1,852,452 - RR42_RS28945 0.13 +0.1 1,852,452 - RR42_RS28945 0.13 -0.8 1,852,452 - RR42_RS28945 0.13 +0.9 1,852,452 - RR42_RS28945 0.13 +0.4 1,852,474 - RR42_RS28945 0.15 +0.1 1,852,481 + RR42_RS28945 0.16 +0.0 1,852,482 - RR42_RS28945 0.16 +2.0 1,852,490 - RR42_RS28945 0.17 -0.4 1,852,636 + RR42_RS28945 0.35 -1.0 1,852,637 - RR42_RS28945 0.35 +0.5 1,852,652 + RR42_RS28945 0.37 -0.4 1,852,937 + RR42_RS28945 0.71 -1.2 1,852,938 - RR42_RS28945 0.71 +0.3 1,852,946 - -0.7 1,852,965 + RR42_RS28945 0.74 -0.6 1,852,998 - RR42_RS28945 0.78 -0.5 1,853,132 + +0.1 1,853,132 + -0.9 1,853,132 + +0.1 1,853,132 + -2.1 1,853,132 + -0.7 1,853,132 + +1.1 1,853,132 + -0.0 1,853,133 - +0.9 1,853,133 - -0.4 1,853,133 - +0.3 1,853,133 - +0.9 1,853,133 - -0.2 1,853,133 - -1.4 1,853,133 - -0.2 1,853,133 - +0.0 1,853,133 - -0.4 1,853,133 - +0.6 1,853,153 + -0.1 1,853,154 - +0.5 1,853,169 + +0.2 1,853,169 + -1.0 1,853,169 + +0.2 1,853,169 + +0.3 1,853,169 + -0.9 1,853,169 + -1.0 1,853,169 + -0.7 1,853,169 + -0.9 1,853,170 - +0.2 1,853,170 - -0.3 1,853,170 - +0.7 1,853,170 - +0.1 1,853,207 + +0.6 1,853,207 + -1.0 1,853,207 + +0.1 1,853,207 + +0.1 1,853,208 - +0.7 1,853,208 - -0.8 1,853,280 + +0.5 1,853,280 + +1.1 1,853,414 - -0.1 1,853,476 + -0.0 1,853,476 + +1.2 1,853,477 - -1.6 1,853,547 + RR42_RS28950 0.11 -0.9 1,853,738 - RR42_RS28950 0.27 -1.3 1,853,842 + RR42_RS28950 0.36 -1.2 1,853,842 + RR42_RS28950 0.36 +0.7 1,853,843 - RR42_RS28950 0.36 -1.2 1,853,843 - RR42_RS28950 0.36 +0.2
Or see this region's nucleotide sequence