Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28800 and RR42_RS28805 are separated by 118 nucleotides RR42_RS28805 and RR42_RS28810 are separated by 262 nucleotides RR42_RS28810 and RR42_RS28815 are separated by 62 nucleotides
RR42_RS28800: RR42_RS28800 - isocitrate dehydrogenase, at 1,812,153 to 1,814,390
_RS28800
RR42_RS28805: RR42_RS28805 - hypothetical protein, at 1,814,509 to 1,815,510
_RS28805
RR42_RS28810: RR42_RS28810 - RNA polymerase sigma factor, at 1,815,773 to 1,816,282
_RS28810
RR42_RS28815: RR42_RS28815 - iron dicitrate transport regulator FecR, at 1,816,345 to 1,817,334
_RS28815
Position (kb)
1814
1815
1816 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1813.675 kb on - strand, within RR42_RS28800 at 1813.675 kb on - strand, within RR42_RS28800 at 1813.675 kb on - strand, within RR42_RS28800 at 1813.806 kb on + strand, within RR42_RS28800 at 1813.893 kb on + strand, within RR42_RS28800 at 1813.893 kb on + strand, within RR42_RS28800 at 1813.894 kb on - strand, within RR42_RS28800 at 1813.894 kb on - strand, within RR42_RS28800 at 1813.894 kb on - strand, within RR42_RS28800 at 1814.145 kb on + strand, within RR42_RS28800 at 1814.157 kb on + strand, within RR42_RS28800 at 1814.157 kb on + strand, within RR42_RS28800 at 1814.307 kb on + strand at 1814.307 kb on + strand at 1814.307 kb on + strand at 1814.307 kb on + strand at 1814.307 kb on + strand at 1814.307 kb on + strand at 1814.308 kb on - strand at 1814.308 kb on - strand at 1814.388 kb on + strand at 1814.495 kb on - strand at 1814.549 kb on + strand at 1814.557 kb on + strand at 1814.557 kb on + strand at 1814.557 kb on + strand at 1814.846 kb on + strand, within RR42_RS28805 at 1814.931 kb on - strand, within RR42_RS28805 at 1814.931 kb on - strand, within RR42_RS28805 at 1815.017 kb on + strand, within RR42_RS28805 at 1815.017 kb on + strand, within RR42_RS28805 at 1815.104 kb on - strand, within RR42_RS28805 at 1815.104 kb on - strand, within RR42_RS28805 at 1815.221 kb on + strand, within RR42_RS28805 at 1815.221 kb on + strand, within RR42_RS28805 at 1815.222 kb on - strand, within RR42_RS28805 at 1815.222 kb on - strand, within RR42_RS28805 at 1815.293 kb on - strand, within RR42_RS28805 at 1815.324 kb on + strand, within RR42_RS28805 at 1815.564 kb on - strand at 1815.575 kb on - strand at 1815.575 kb on - strand at 1815.579 kb on - strand at 1815.606 kb on + strand at 1815.607 kb on - strand at 1815.607 kb on - strand at 1815.639 kb on - strand at 1815.639 kb on - strand at 1815.639 kb on - strand at 1815.691 kb on + strand at 1815.695 kb on + strand at 1815.696 kb on - strand at 1815.709 kb on + strand at 1815.712 kb on - strand at 1815.758 kb on + strand at 1815.795 kb on + strand at 1815.795 kb on + strand at 1815.796 kb on - strand at 1815.796 kb on - strand at 1815.956 kb on + strand, within RR42_RS28810 at 1815.957 kb on - strand, within RR42_RS28810 at 1816.233 kb on - strand at 1816.259 kb on + strand at 1816.259 kb on + strand at 1816.260 kb on - strand at 1816.260 kb on - strand at 1816.260 kb on - strand at 1816.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,813,675 - RR42_RS28800 0.68 +0.6 1,813,675 - RR42_RS28800 0.68 -0.9 1,813,675 - RR42_RS28800 0.68 +0.7 1,813,806 + RR42_RS28800 0.74 +0.0 1,813,893 + RR42_RS28800 0.78 -0.2 1,813,893 + RR42_RS28800 0.78 +0.6 1,813,894 - RR42_RS28800 0.78 -0.2 1,813,894 - RR42_RS28800 0.78 +1.9 1,813,894 - RR42_RS28800 0.78 -0.1 1,814,145 + RR42_RS28800 0.89 -0.7 1,814,157 + RR42_RS28800 0.90 -1.2 1,814,157 + RR42_RS28800 0.90 +0.7 1,814,307 + +0.7 1,814,307 + -0.5 1,814,307 + +0.4 1,814,307 + -0.7 1,814,307 + +1.6 1,814,307 + +0.1 1,814,308 - -0.7 1,814,308 - -0.5 1,814,388 + -1.1 1,814,495 - +0.2 1,814,549 + -1.0 1,814,557 + +1.0 1,814,557 + +0.1 1,814,557 + -1.3 1,814,846 + RR42_RS28805 0.34 -1.1 1,814,931 - RR42_RS28805 0.42 +0.8 1,814,931 - RR42_RS28805 0.42 +0.9 1,815,017 + RR42_RS28805 0.51 -0.7 1,815,017 + RR42_RS28805 0.51 -0.2 1,815,104 - RR42_RS28805 0.59 -0.3 1,815,104 - RR42_RS28805 0.59 -0.8 1,815,221 + RR42_RS28805 0.71 -0.1 1,815,221 + RR42_RS28805 0.71 +1.5 1,815,222 - RR42_RS28805 0.71 -0.3 1,815,222 - RR42_RS28805 0.71 -0.8 1,815,293 - RR42_RS28805 0.78 -1.6 1,815,324 + RR42_RS28805 0.81 -0.1 1,815,564 - -1.6 1,815,575 - -0.9 1,815,575 - -0.2 1,815,579 - -0.2 1,815,606 + -0.7 1,815,607 - -0.1 1,815,607 - +0.0 1,815,639 - +1.3 1,815,639 - -0.2 1,815,639 - +0.3 1,815,691 + +0.9 1,815,695 + +0.2 1,815,696 - +0.4 1,815,709 + +0.4 1,815,712 - -0.4 1,815,758 + -1.1 1,815,795 + -0.1 1,815,795 + +1.1 1,815,796 - -0.0 1,815,796 - -0.4 1,815,956 + RR42_RS28810 0.36 -1.9 1,815,957 - RR42_RS28810 0.36 -1.9 1,816,233 - -0.0 1,816,259 + -0.4 1,816,259 + -1.7 1,816,260 - -1.3 1,816,260 - -0.3 1,816,260 - -0.5 1,816,324 - -0.9
Or see this region's nucleotide sequence