Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28715 and RR42_RS28720 are separated by 140 nucleotides RR42_RS28720 and RR42_RS28725 are separated by 285 nucleotides
RR42_RS28715: RR42_RS28715 - aldo/keto reductase, at 1,794,013 to 1,794,948
_RS28715
RR42_RS28720: RR42_RS28720 - hypothetical protein, at 1,795,089 to 1,795,553
_RS28720
RR42_RS28725: RR42_RS28725 - ABC transporter permease, at 1,795,839 to 1,796,705
_RS28725
Position (kb)
1795
1796 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1794.338 kb on + strand, within RR42_RS28715 at 1794.506 kb on - strand, within RR42_RS28715 at 1794.506 kb on - strand, within RR42_RS28715 at 1794.526 kb on - strand, within RR42_RS28715 at 1794.586 kb on + strand, within RR42_RS28715 at 1794.587 kb on - strand, within RR42_RS28715 at 1794.665 kb on + strand, within RR42_RS28715 at 1794.666 kb on - strand, within RR42_RS28715 at 1794.666 kb on - strand, within RR42_RS28715 at 1794.666 kb on - strand, within RR42_RS28715 at 1794.777 kb on + strand, within RR42_RS28715 at 1794.778 kb on - strand, within RR42_RS28715 at 1794.778 kb on - strand, within RR42_RS28715 at 1794.891 kb on + strand at 1794.891 kb on + strand at 1794.892 kb on - strand at 1795.001 kb on + strand at 1795.002 kb on - strand at 1795.036 kb on + strand at 1795.061 kb on + strand at 1795.061 kb on + strand at 1795.061 kb on + strand at 1795.062 kb on - strand at 1795.073 kb on + strand at 1795.074 kb on - strand at 1795.116 kb on + strand at 1795.259 kb on + strand, within RR42_RS28720 at 1795.353 kb on - strand, within RR42_RS28720 at 1795.491 kb on + strand, within RR42_RS28720 at 1795.531 kb on - strand at 1795.531 kb on - strand at 1795.881 kb on + strand at 1795.882 kb on - strand at 1795.882 kb on - strand at 1795.882 kb on - strand at 1795.909 kb on + strand at 1796.041 kb on + strand, within RR42_RS28725 at 1796.175 kb on + strand, within RR42_RS28725 at 1796.175 kb on + strand, within RR42_RS28725 at 1796.175 kb on + strand, within RR42_RS28725 at 1796.176 kb on - strand, within RR42_RS28725 at 1796.176 kb on - strand, within RR42_RS28725 at 1796.176 kb on - strand, within RR42_RS28725 at 1796.408 kb on - strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.514 kb on + strand, within RR42_RS28725 at 1796.514 kb on + strand, within RR42_RS28725 at 1796.514 kb on + strand, within RR42_RS28725 at 1796.515 kb on - strand, within RR42_RS28725 at 1796.515 kb on - strand, within RR42_RS28725 at 1796.515 kb on - strand, within RR42_RS28725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,794,338 + RR42_RS28715 0.35 +0.2 1,794,506 - RR42_RS28715 0.53 -0.3 1,794,506 - RR42_RS28715 0.53 -0.7 1,794,526 - RR42_RS28715 0.55 -0.8 1,794,586 + RR42_RS28715 0.61 +1.1 1,794,587 - RR42_RS28715 0.61 -0.1 1,794,665 + RR42_RS28715 0.70 +0.2 1,794,666 - RR42_RS28715 0.70 +0.2 1,794,666 - RR42_RS28715 0.70 -0.2 1,794,666 - RR42_RS28715 0.70 +0.2 1,794,777 + RR42_RS28715 0.82 +0.4 1,794,778 - RR42_RS28715 0.82 +0.2 1,794,778 - RR42_RS28715 0.82 -0.1 1,794,891 + -0.1 1,794,891 + +1.6 1,794,892 - -0.5 1,795,001 + +0.7 1,795,002 - -0.0 1,795,036 + +0.9 1,795,061 + -0.3 1,795,061 + -0.2 1,795,061 + +0.2 1,795,062 - +0.4 1,795,073 + +0.1 1,795,074 - +2.2 1,795,116 + +1.1 1,795,259 + RR42_RS28720 0.37 -1.4 1,795,353 - RR42_RS28720 0.57 -1.0 1,795,491 + RR42_RS28720 0.86 -0.1 1,795,531 - +0.6 1,795,531 - +0.4 1,795,881 + +0.7 1,795,882 - +0.2 1,795,882 - -1.0 1,795,882 - +0.1 1,795,909 + -0.7 1,796,041 + RR42_RS28725 0.23 -1.2 1,796,175 + RR42_RS28725 0.39 +0.2 1,796,175 + RR42_RS28725 0.39 -0.3 1,796,175 + RR42_RS28725 0.39 +0.9 1,796,176 - RR42_RS28725 0.39 +0.7 1,796,176 - RR42_RS28725 0.39 -0.2 1,796,176 - RR42_RS28725 0.39 +0.5 1,796,408 - RR42_RS28725 0.66 -0.9 1,796,505 + RR42_RS28725 0.77 -0.5 1,796,505 + RR42_RS28725 0.77 +0.1 1,796,505 + RR42_RS28725 0.77 +0.0 1,796,505 + RR42_RS28725 0.77 +0.2 1,796,506 - RR42_RS28725 0.77 -0.2 1,796,506 - RR42_RS28725 0.77 +0.9 1,796,506 - RR42_RS28725 0.77 -0.0 1,796,506 - RR42_RS28725 0.77 +0.4 1,796,506 - RR42_RS28725 0.77 -0.2 1,796,514 + RR42_RS28725 0.78 -1.1 1,796,514 + RR42_RS28725 0.78 -0.9 1,796,514 + RR42_RS28725 0.78 -0.5 1,796,515 - RR42_RS28725 0.78 +0.5 1,796,515 - RR42_RS28725 0.78 +0.1 1,796,515 - RR42_RS28725 0.78 -2.0
Or see this region's nucleotide sequence