Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28615 and RR42_RS28620 are separated by 3 nucleotides RR42_RS28620 and RR42_RS28625 overlap by 7 nucleotides RR42_RS28625 and RR42_RS28630 overlap by 20 nucleotides
RR42_RS28615: RR42_RS28615 - ABC transporter substrate-binding protein, at 1,776,353 to 1,777,297
_RS28615
RR42_RS28620: RR42_RS28620 - ABC transporter, at 1,777,301 to 1,778,131
_RS28620
RR42_RS28625: RR42_RS28625 - ABC transporter permease, at 1,778,125 to 1,779,159
_RS28625
RR42_RS28630: RR42_RS28630 - ABC transporter substrate-binding protein, at 1,779,140 to 1,780,180
_RS28630
Position (kb)
1778
1779
1780 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1777.302 kb on + strand at 1777.302 kb on + strand at 1777.302 kb on + strand at 1777.302 kb on + strand at 1777.303 kb on - strand at 1777.303 kb on - strand at 1777.303 kb on - strand at 1777.665 kb on + strand, within RR42_RS28620 at 1777.792 kb on - strand, within RR42_RS28620 at 1778.049 kb on - strand at 1778.049 kb on - strand at 1778.110 kb on + strand at 1778.231 kb on + strand, within RR42_RS28625 at 1778.273 kb on + strand, within RR42_RS28625 at 1778.273 kb on + strand, within RR42_RS28625 at 1778.441 kb on + strand, within RR42_RS28625 at 1778.441 kb on + strand at 1778.442 kb on - strand, within RR42_RS28625 at 1778.442 kb on - strand, within RR42_RS28625 at 1778.741 kb on + strand, within RR42_RS28625 at 1778.848 kb on + strand, within RR42_RS28625 at 1778.849 kb on - strand, within RR42_RS28625 at 1778.890 kb on + strand, within RR42_RS28625 at 1778.891 kb on - strand, within RR42_RS28625 at 1779.159 kb on + strand at 1779.159 kb on + strand at 1779.159 kb on + strand at 1779.159 kb on + strand at 1779.160 kb on - strand at 1779.480 kb on + strand, within RR42_RS28630 at 1779.481 kb on - strand, within RR42_RS28630 at 1779.481 kb on - strand, within RR42_RS28630 at 1779.537 kb on + strand, within RR42_RS28630 at 1779.538 kb on - strand, within RR42_RS28630 at 1779.832 kb on + strand, within RR42_RS28630 at 1779.832 kb on + strand, within RR42_RS28630 at 1779.832 kb on + strand, within RR42_RS28630 at 1780.109 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,777,302 + +0.1 1,777,302 + -0.3 1,777,302 + +0.3 1,777,302 + +0.3 1,777,303 - -0.1 1,777,303 - -0.1 1,777,303 - -0.6 1,777,665 + RR42_RS28620 0.44 -0.6 1,777,792 - RR42_RS28620 0.59 -0.1 1,778,049 - +0.2 1,778,049 - -1.6 1,778,110 + -0.2 1,778,231 + RR42_RS28625 0.10 -0.4 1,778,273 + RR42_RS28625 0.14 +2.8 1,778,273 + RR42_RS28625 0.14 +0.1 1,778,441 + RR42_RS28625 0.31 -0.1 1,778,441 + +0.9 1,778,442 - RR42_RS28625 0.31 -0.1 1,778,442 - RR42_RS28625 0.31 -0.2 1,778,741 + RR42_RS28625 0.60 +1.7 1,778,848 + RR42_RS28625 0.70 -0.7 1,778,849 - RR42_RS28625 0.70 -0.3 1,778,890 + RR42_RS28625 0.74 +0.6 1,778,891 - RR42_RS28625 0.74 -0.6 1,779,159 + -1.8 1,779,159 + +0.2 1,779,159 + -0.1 1,779,159 + +0.6 1,779,160 - +0.9 1,779,480 + RR42_RS28630 0.33 +0.3 1,779,481 - RR42_RS28630 0.33 -0.2 1,779,481 - RR42_RS28630 0.33 +0.7 1,779,537 + RR42_RS28630 0.38 -0.3 1,779,538 - RR42_RS28630 0.38 -0.1 1,779,832 + RR42_RS28630 0.66 -0.6 1,779,832 + RR42_RS28630 0.66 -0.1 1,779,832 + RR42_RS28630 0.66 +1.2 1,780,109 + -0.1
Or see this region's nucleotide sequence