Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28530 and RR42_RS28535 are separated by 127 nucleotides RR42_RS28535 and RR42_RS28540 are separated by 131 nucleotides RR42_RS28540 and RR42_RS28545 are separated by 60 nucleotides
RR42_RS28530: RR42_RS28530 - FAD-binding protein, at 1,756,308 to 1,757,126
_RS28530
RR42_RS28535: RR42_RS28535 - transcriptional regulator, at 1,757,254 to 1,757,712
_RS28535
RR42_RS28540: RR42_RS28540 - glutamate--pyruvate aminotransferase, at 1,757,844 to 1,759,100
_RS28540
RR42_RS28545: RR42_RS28545 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 1,759,161 to 1,760,315
_RS28545
Position (kb)
1757
1758
1759
1760 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1756.958 kb on + strand, within RR42_RS28530 at 1756.958 kb on + strand, within RR42_RS28530 at 1756.959 kb on - strand, within RR42_RS28530 at 1756.980 kb on + strand, within RR42_RS28530 at 1756.981 kb on - strand, within RR42_RS28530 at 1757.028 kb on + strand, within RR42_RS28530 at 1757.079 kb on + strand at 1757.080 kb on - strand at 1757.254 kb on - strand at 1757.495 kb on - strand, within RR42_RS28535 at 1757.495 kb on - strand, within RR42_RS28535 at 1757.569 kb on + strand, within RR42_RS28535 at 1757.569 kb on + strand, within RR42_RS28535 at 1757.569 kb on + strand, within RR42_RS28535 at 1757.569 kb on + strand, within RR42_RS28535 at 1757.569 kb on + strand, within RR42_RS28535 at 1757.570 kb on - strand, within RR42_RS28535 at 1757.570 kb on - strand, within RR42_RS28535 at 1757.570 kb on - strand, within RR42_RS28535 at 1757.570 kb on - strand, within RR42_RS28535 at 1757.711 kb on - strand at 1757.826 kb on + strand at 1757.826 kb on + strand at 1757.827 kb on - strand at 1757.899 kb on - strand at 1758.002 kb on + strand, within RR42_RS28540 at 1758.052 kb on - strand, within RR42_RS28540 at 1758.238 kb on - strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.249 kb on - strand, within RR42_RS28540 at 1758.249 kb on - strand, within RR42_RS28540 at 1758.249 kb on - strand, within RR42_RS28540 at 1758.252 kb on + strand, within RR42_RS28540 at 1758.253 kb on - strand, within RR42_RS28540 at 1758.348 kb on + strand, within RR42_RS28540 at 1758.349 kb on - strand, within RR42_RS28540 at 1758.349 kb on - strand, within RR42_RS28540 at 1758.456 kb on - strand, within RR42_RS28540 at 1758.456 kb on - strand, within RR42_RS28540 at 1758.657 kb on + strand, within RR42_RS28540 at 1758.666 kb on + strand, within RR42_RS28540 at 1758.667 kb on - strand, within RR42_RS28540 at 1758.750 kb on + strand, within RR42_RS28540 at 1758.750 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.829 kb on - strand, within RR42_RS28540 at 1758.829 kb on - strand, within RR42_RS28540 at 1758.831 kb on - strand, within RR42_RS28540 at 1758.942 kb on + strand, within RR42_RS28540 at 1758.943 kb on - strand, within RR42_RS28540 at 1758.954 kb on + strand, within RR42_RS28540 at 1759.112 kb on + strand at 1759.112 kb on + strand at 1759.113 kb on - strand at 1759.460 kb on + strand, within RR42_RS28545 at 1759.780 kb on + strand, within RR42_RS28545 at 1759.780 kb on + strand, within RR42_RS28545 at 1759.781 kb on - strand, within RR42_RS28545 at 1759.781 kb on - strand, within RR42_RS28545 at 1759.781 kb on - strand, within RR42_RS28545 at 1759.970 kb on - strand, within RR42_RS28545 at 1760.050 kb on + strand, within RR42_RS28545 at 1760.051 kb on - strand, within RR42_RS28545
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,756,958 + RR42_RS28530 0.79 -0.4 1,756,958 + RR42_RS28530 0.79 +1.5 1,756,959 - RR42_RS28530 0.79 +1.9 1,756,980 + RR42_RS28530 0.82 +0.5 1,756,981 - RR42_RS28530 0.82 +0.0 1,757,028 + RR42_RS28530 0.88 +0.9 1,757,079 + -0.7 1,757,080 - -0.8 1,757,254 - -0.9 1,757,495 - RR42_RS28535 0.53 -0.2 1,757,495 - RR42_RS28535 0.53 +0.1 1,757,569 + RR42_RS28535 0.69 +1.7 1,757,569 + RR42_RS28535 0.69 +0.5 1,757,569 + RR42_RS28535 0.69 -0.5 1,757,569 + RR42_RS28535 0.69 +0.4 1,757,569 + RR42_RS28535 0.69 -0.7 1,757,570 - RR42_RS28535 0.69 +0.2 1,757,570 - RR42_RS28535 0.69 +0.4 1,757,570 - RR42_RS28535 0.69 -0.2 1,757,570 - RR42_RS28535 0.69 -0.1 1,757,711 - +0.1 1,757,826 + +0.4 1,757,826 + +0.2 1,757,827 - +0.4 1,757,899 - +0.6 1,758,002 + RR42_RS28540 0.13 +0.9 1,758,052 - RR42_RS28540 0.17 +1.9 1,758,238 - RR42_RS28540 0.31 +0.9 1,758,248 + RR42_RS28540 0.32 -2.3 1,758,248 + RR42_RS28540 0.32 +0.4 1,758,248 + RR42_RS28540 0.32 +0.1 1,758,248 + RR42_RS28540 0.32 +0.9 1,758,248 + RR42_RS28540 0.32 -1.6 1,758,249 - RR42_RS28540 0.32 +0.8 1,758,249 - RR42_RS28540 0.32 -1.0 1,758,249 - RR42_RS28540 0.32 +0.5 1,758,252 + RR42_RS28540 0.32 +4.5 1,758,253 - RR42_RS28540 0.33 +0.2 1,758,348 + RR42_RS28540 0.40 +1.1 1,758,349 - RR42_RS28540 0.40 +0.7 1,758,349 - RR42_RS28540 0.40 -0.1 1,758,456 - RR42_RS28540 0.49 +0.8 1,758,456 - RR42_RS28540 0.49 -0.4 1,758,657 + RR42_RS28540 0.65 -1.9 1,758,666 + RR42_RS28540 0.65 +0.6 1,758,667 - RR42_RS28540 0.65 +0.3 1,758,750 + RR42_RS28540 0.72 +0.4 1,758,750 + RR42_RS28540 0.72 +0.3 1,758,828 + RR42_RS28540 0.78 -0.8 1,758,828 + RR42_RS28540 0.78 -2.9 1,758,828 + RR42_RS28540 0.78 +1.9 1,758,828 + RR42_RS28540 0.78 +0.2 1,758,829 - RR42_RS28540 0.78 +1.5 1,758,829 - RR42_RS28540 0.78 +0.3 1,758,831 - RR42_RS28540 0.79 -1.1 1,758,942 + RR42_RS28540 0.87 +0.1 1,758,943 - RR42_RS28540 0.87 -0.4 1,758,954 + RR42_RS28540 0.88 -0.7 1,759,112 + -0.5 1,759,112 + -0.1 1,759,113 - -0.1 1,759,460 + RR42_RS28545 0.26 +1.1 1,759,780 + RR42_RS28545 0.54 +0.9 1,759,780 + RR42_RS28545 0.54 +1.3 1,759,781 - RR42_RS28545 0.54 +0.9 1,759,781 - RR42_RS28545 0.54 +0.8 1,759,781 - RR42_RS28545 0.54 +0.9 1,759,970 - RR42_RS28545 0.70 -0.0 1,760,050 + RR42_RS28545 0.77 -0.6 1,760,051 - RR42_RS28545 0.77 -0.3
Or see this region's nucleotide sequence