Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27765 and RR42_RS27770 are separated by 114 nucleotides RR42_RS27770 and RR42_RS27775 are separated by 29 nucleotides RR42_RS27775 and RR42_RS27780 are separated by 133 nucleotides
RR42_RS27765: RR42_RS27765 - haloacid dehalogenase, at 1,592,002 to 1,592,625
_RS27765
RR42_RS27770: RR42_RS27770 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,592,740 to 1,593,372
_RS27770
RR42_RS27775: RR42_RS27775 - phospholipase, at 1,593,402 to 1,595,606
_RS27775
RR42_RS27780: RR42_RS27780 - betaine-aldehyde dehydrogenase, at 1,595,740 to 1,597,191
_RS27780
Position (kb)
1593
1594
1595
1596 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1592.444 kb on - strand, within RR42_RS27765 at 1592.860 kb on + strand, within RR42_RS27770 at 1592.861 kb on - strand, within RR42_RS27770 at 1592.861 kb on - strand, within RR42_RS27770 at 1593.263 kb on - strand, within RR42_RS27770 at 1593.559 kb on + strand at 1593.646 kb on + strand, within RR42_RS27775 at 1593.762 kb on - strand, within RR42_RS27775 at 1593.767 kb on + strand, within RR42_RS27775 at 1593.897 kb on - strand, within RR42_RS27775 at 1593.898 kb on + strand, within RR42_RS27775 at 1593.898 kb on + strand, within RR42_RS27775 at 1593.935 kb on - strand, within RR42_RS27775 at 1593.985 kb on - strand, within RR42_RS27775 at 1594.103 kb on + strand, within RR42_RS27775 at 1594.295 kb on + strand, within RR42_RS27775 at 1594.296 kb on - strand, within RR42_RS27775 at 1594.296 kb on - strand, within RR42_RS27775 at 1594.296 kb on - strand, within RR42_RS27775 at 1594.296 kb on - strand, within RR42_RS27775 at 1594.394 kb on - strand, within RR42_RS27775 at 1594.439 kb on - strand, within RR42_RS27775 at 1594.510 kb on + strand, within RR42_RS27775 at 1594.610 kb on + strand, within RR42_RS27775 at 1594.610 kb on + strand, within RR42_RS27775 at 1594.611 kb on - strand, within RR42_RS27775 at 1594.611 kb on - strand, within RR42_RS27775 at 1594.637 kb on - strand, within RR42_RS27775 at 1594.879 kb on + strand, within RR42_RS27775 at 1594.879 kb on + strand, within RR42_RS27775 at 1594.880 kb on - strand, within RR42_RS27775 at 1594.880 kb on - strand, within RR42_RS27775 at 1594.880 kb on - strand, within RR42_RS27775 at 1594.922 kb on - strand, within RR42_RS27775 at 1594.972 kb on + strand, within RR42_RS27775 at 1594.972 kb on + strand, within RR42_RS27775 at 1594.972 kb on + strand, within RR42_RS27775 at 1594.973 kb on - strand, within RR42_RS27775 at 1594.973 kb on - strand, within RR42_RS27775 at 1594.973 kb on - strand, within RR42_RS27775 at 1595.077 kb on + strand, within RR42_RS27775 at 1595.078 kb on - strand, within RR42_RS27775 at 1595.093 kb on + strand, within RR42_RS27775 at 1595.143 kb on + strand, within RR42_RS27775 at 1595.462 kb on + strand at 1595.591 kb on + strand at 1595.591 kb on + strand at 1595.591 kb on + strand at 1595.592 kb on - strand at 1595.673 kb on - strand at 1595.692 kb on - strand at 1595.692 kb on - strand at 1595.692 kb on - strand at 1595.741 kb on + strand at 1595.742 kb on - strand at 1595.851 kb on + strand at 1595.867 kb on + strand at 1595.867 kb on + strand at 1595.867 kb on + strand at 1595.867 kb on + strand at 1595.867 kb on + strand at 1595.868 kb on - strand at 1595.868 kb on - strand at 1595.868 kb on - strand at 1596.095 kb on + strand, within RR42_RS27780 at 1596.095 kb on + strand, within RR42_RS27780 at 1596.095 kb on + strand, within RR42_RS27780 at 1596.096 kb on - strand, within RR42_RS27780 at 1596.098 kb on + strand, within RR42_RS27780 at 1596.167 kb on + strand, within RR42_RS27780 at 1596.167 kb on + strand, within RR42_RS27780 at 1596.167 kb on + strand, within RR42_RS27780 at 1596.168 kb on - strand, within RR42_RS27780 at 1596.350 kb on + strand, within RR42_RS27780
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,592,444 - RR42_RS27765 0.71 -0.2 1,592,860 + RR42_RS27770 0.19 -0.3 1,592,861 - RR42_RS27770 0.19 -0.4 1,592,861 - RR42_RS27770 0.19 +0.0 1,593,263 - RR42_RS27770 0.83 -0.5 1,593,559 + -0.1 1,593,646 + RR42_RS27775 0.11 -0.1 1,593,762 - RR42_RS27775 0.16 -0.3 1,593,767 + RR42_RS27775 0.17 +0.5 1,593,897 - RR42_RS27775 0.22 -1.2 1,593,898 + RR42_RS27775 0.22 -0.7 1,593,898 + RR42_RS27775 0.22 +1.2 1,593,935 - RR42_RS27775 0.24 -0.1 1,593,985 - RR42_RS27775 0.26 +0.0 1,594,103 + RR42_RS27775 0.32 +0.3 1,594,295 + RR42_RS27775 0.40 -0.3 1,594,296 - RR42_RS27775 0.41 -0.6 1,594,296 - RR42_RS27775 0.41 -0.1 1,594,296 - RR42_RS27775 0.41 +0.1 1,594,296 - RR42_RS27775 0.41 -0.6 1,594,394 - RR42_RS27775 0.45 +1.4 1,594,439 - RR42_RS27775 0.47 -0.4 1,594,510 + RR42_RS27775 0.50 -1.2 1,594,610 + RR42_RS27775 0.55 -1.2 1,594,610 + RR42_RS27775 0.55 -0.4 1,594,611 - RR42_RS27775 0.55 +0.7 1,594,611 - RR42_RS27775 0.55 +1.4 1,594,637 - RR42_RS27775 0.56 -0.5 1,594,879 + RR42_RS27775 0.67 +0.1 1,594,879 + RR42_RS27775 0.67 -0.6 1,594,880 - RR42_RS27775 0.67 -0.2 1,594,880 - RR42_RS27775 0.67 +0.3 1,594,880 - RR42_RS27775 0.67 +0.9 1,594,922 - RR42_RS27775 0.69 +0.8 1,594,972 + RR42_RS27775 0.71 -0.4 1,594,972 + RR42_RS27775 0.71 +0.6 1,594,972 + RR42_RS27775 0.71 +0.3 1,594,973 - RR42_RS27775 0.71 -0.6 1,594,973 - RR42_RS27775 0.71 -0.4 1,594,973 - RR42_RS27775 0.71 -0.4 1,595,077 + RR42_RS27775 0.76 -0.1 1,595,078 - RR42_RS27775 0.76 +0.9 1,595,093 + RR42_RS27775 0.77 -1.5 1,595,143 + RR42_RS27775 0.79 -0.8 1,595,462 + -1.6 1,595,591 + +0.1 1,595,591 + +0.6 1,595,591 + -0.3 1,595,592 - +0.3 1,595,673 - -0.1 1,595,692 - +0.8 1,595,692 - +1.5 1,595,692 - +0.3 1,595,741 + +0.8 1,595,742 - -0.6 1,595,851 + -1.4 1,595,867 + +1.2 1,595,867 + -0.7 1,595,867 + -1.0 1,595,867 + -0.8 1,595,867 + -1.1 1,595,868 - +0.8 1,595,868 - -2.2 1,595,868 - +0.0 1,596,095 + RR42_RS27780 0.24 -1.1 1,596,095 + RR42_RS27780 0.24 +0.5 1,596,095 + RR42_RS27780 0.24 +0.4 1,596,096 - RR42_RS27780 0.25 -0.6 1,596,098 + RR42_RS27780 0.25 +0.4 1,596,167 + RR42_RS27780 0.29 -0.4 1,596,167 + RR42_RS27780 0.29 -1.4 1,596,167 + RR42_RS27780 0.29 -0.4 1,596,168 - RR42_RS27780 0.29 -0.1 1,596,350 + RR42_RS27780 0.42 +3.3
Or see this region's nucleotide sequence