Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27035 and RR42_RS27040 are separated by 3 nucleotides RR42_RS27040 and RR42_RS27045 are separated by 87 nucleotides RR42_RS27045 and RR42_RS27050 are separated by 80 nucleotides RR42_RS27050 and RR42_RS27055 are separated by 10 nucleotides
RR42_RS27035: RR42_RS27035 - hypothetical protein, at 1,418,652 to 1,418,831
_RS27035
RR42_RS27040: RR42_RS27040 - hypothetical protein, at 1,418,835 to 1,419,020
_RS27040
RR42_RS27045: RR42_RS27045 - hypothetical protein, at 1,419,108 to 1,419,506
_RS27045
RR42_RS27050: RR42_RS27050 - transporter, at 1,419,587 to 1,420,960
_RS27050
RR42_RS27055: RR42_RS27055 - membrane protein, at 1,420,971 to 1,424,111
_RS27055
Position (kb) 1419
1420
1421 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1418.616 kb on + strand at 1418.616 kb on + strand at 1418.617 kb on - strand at 1418.617 kb on - strand at 1418.621 kb on + strand at 1418.621 kb on + strand at 1418.744 kb on + strand, within RR42_RS27035 at 1418.745 kb on - strand, within RR42_RS27035 at 1418.751 kb on - strand, within RR42_RS27035 at 1418.751 kb on - strand, within RR42_RS27035 at 1419.317 kb on - strand, within RR42_RS27045 at 1419.376 kb on + strand, within RR42_RS27045 at 1419.417 kb on + strand, within RR42_RS27045 at 1419.537 kb on + strand at 1419.663 kb on + strand at 1419.663 kb on + strand at 1419.664 kb on - strand at 1419.664 kb on - strand at 1419.672 kb on + strand at 1419.673 kb on - strand at 1420.206 kb on + strand, within RR42_RS27050 at 1420.507 kb on - strand, within RR42_RS27050 at 1420.683 kb on + strand, within RR42_RS27050 at 1420.683 kb on + strand, within RR42_RS27050 at 1420.684 kb on - strand, within RR42_RS27050 at 1421.077 kb on + strand at 1421.078 kb on - strand at 1421.876 kb on - strand, within RR42_RS27055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,418,616 + -0.1 1,418,616 + +1.2 1,418,617 - -0.4 1,418,617 - -0.1 1,418,621 + -0.5 1,418,621 + -0.3 1,418,744 + RR42_RS27035 0.51 +0.4 1,418,745 - RR42_RS27035 0.52 +0.3 1,418,751 - RR42_RS27035 0.55 +1.6 1,418,751 - RR42_RS27035 0.55 +0.4 1,419,317 - RR42_RS27045 0.52 +0.4 1,419,376 + RR42_RS27045 0.67 +0.7 1,419,417 + RR42_RS27045 0.77 -1.8 1,419,537 + -0.8 1,419,663 + -0.8 1,419,663 + +0.4 1,419,664 - -1.9 1,419,664 - +1.3 1,419,672 + -0.5 1,419,673 - +0.2 1,420,206 + RR42_RS27050 0.45 +1.0 1,420,507 - RR42_RS27050 0.67 -0.3 1,420,683 + RR42_RS27050 0.80 +0.6 1,420,683 + RR42_RS27050 0.80 +0.6 1,420,684 - RR42_RS27050 0.80 +2.4 1,421,077 + -0.4 1,421,078 - -0.6 1,421,876 - RR42_RS27055 0.29 -0.6
Or see this region's nucleotide sequence