Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25950 and RR42_RS25955 are separated by 38 nucleotides RR42_RS25955 and RR42_RS25960 are separated by 359 nucleotides RR42_RS25960 and RR42_RS25965 are separated by 305 nucleotides
RR42_RS25950: RR42_RS25950 - hypothetical protein, at 1,164,335 to 1,165,504
_RS25950
RR42_RS25955: RR42_RS25955 - transcriptional regulator, at 1,165,543 to 1,165,941
_RS25955
RR42_RS25960: RR42_RS25960 - hypothetical protein, at 1,166,301 to 1,170,614
_RS25960
RR42_RS25965: RR42_RS25965 - LuxR family transcriptional regulator, at 1,170,920 to 1,171,579
_RS25965
Position (kb)
1166
1167
1168
1169
1170
1171 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1165.312 kb on - strand, within RR42_RS25950 at 1165.332 kb on + strand, within RR42_RS25950 at 1165.332 kb on + strand, within RR42_RS25950 at 1165.353 kb on + strand, within RR42_RS25950 at 1165.354 kb on - strand, within RR42_RS25950 at 1165.354 kb on - strand, within RR42_RS25950 at 1165.354 kb on - strand, within RR42_RS25950 at 1165.937 kb on - strand at 1165.937 kb on - strand at 1166.011 kb on - strand at 1166.038 kb on + strand at 1166.038 kb on + strand at 1166.039 kb on - strand at 1166.039 kb on - strand at 1166.047 kb on - strand at 1166.047 kb on - strand at 1166.190 kb on - strand at 1166.249 kb on + strand at 1166.251 kb on + strand at 1166.251 kb on + strand at 1166.252 kb on - strand at 1166.252 kb on - strand at 1166.252 kb on - strand at 1166.319 kb on + strand at 1166.320 kb on - strand at 1166.820 kb on + strand, within RR42_RS25960 at 1166.821 kb on - strand, within RR42_RS25960 at 1166.821 kb on - strand, within RR42_RS25960 at 1166.880 kb on + strand, within RR42_RS25960 at 1166.902 kb on - strand, within RR42_RS25960 at 1167.363 kb on + strand, within RR42_RS25960 at 1167.423 kb on + strand, within RR42_RS25960 at 1167.423 kb on + strand, within RR42_RS25960 at 1167.423 kb on + strand, within RR42_RS25960 at 1167.423 kb on + strand, within RR42_RS25960 at 1167.424 kb on - strand, within RR42_RS25960 at 1167.424 kb on - strand, within RR42_RS25960 at 1168.029 kb on - strand, within RR42_RS25960 at 1168.176 kb on + strand, within RR42_RS25960 at 1168.176 kb on + strand, within RR42_RS25960 at 1168.177 kb on - strand, within RR42_RS25960 at 1168.230 kb on + strand, within RR42_RS25960 at 1168.301 kb on + strand, within RR42_RS25960 at 1168.301 kb on + strand, within RR42_RS25960 at 1168.301 kb on + strand, within RR42_RS25960 at 1168.301 kb on + strand, within RR42_RS25960 at 1168.301 kb on + strand, within RR42_RS25960 at 1168.301 kb on + strand, within RR42_RS25960 at 1168.301 kb on + strand, within RR42_RS25960 at 1168.302 kb on - strand, within RR42_RS25960 at 1168.302 kb on - strand, within RR42_RS25960 at 1168.302 kb on - strand, within RR42_RS25960 at 1168.302 kb on - strand, within RR42_RS25960 at 1168.416 kb on + strand, within RR42_RS25960 at 1168.417 kb on - strand, within RR42_RS25960 at 1168.417 kb on - strand, within RR42_RS25960 at 1168.417 kb on - strand, within RR42_RS25960 at 1168.538 kb on + strand, within RR42_RS25960 at 1168.560 kb on + strand, within RR42_RS25960 at 1168.560 kb on + strand, within RR42_RS25960 at 1168.560 kb on + strand, within RR42_RS25960 at 1168.561 kb on - strand, within RR42_RS25960 at 1168.561 kb on - strand, within RR42_RS25960 at 1168.684 kb on - strand, within RR42_RS25960 at 1168.768 kb on + strand, within RR42_RS25960 at 1168.769 kb on - strand, within RR42_RS25960 at 1168.783 kb on - strand, within RR42_RS25960 at 1168.803 kb on + strand, within RR42_RS25960 at 1169.097 kb on + strand, within RR42_RS25960 at 1169.098 kb on - strand, within RR42_RS25960 at 1169.154 kb on + strand, within RR42_RS25960 at 1169.155 kb on - strand, within RR42_RS25960 at 1169.306 kb on + strand, within RR42_RS25960 at 1169.310 kb on + strand, within RR42_RS25960 at 1169.310 kb on + strand, within RR42_RS25960 at 1169.310 kb on + strand, within RR42_RS25960 at 1169.310 kb on + strand, within RR42_RS25960 at 1169.310 kb on + strand, within RR42_RS25960 at 1169.310 kb on + strand, within RR42_RS25960 at 1169.311 kb on - strand, within RR42_RS25960 at 1169.311 kb on - strand, within RR42_RS25960 at 1169.311 kb on - strand, within RR42_RS25960 at 1169.311 kb on - strand, within RR42_RS25960 at 1169.312 kb on + strand, within RR42_RS25960 at 1169.312 kb on + strand, within RR42_RS25960 at 1169.380 kb on + strand, within RR42_RS25960 at 1169.381 kb on - strand, within RR42_RS25960 at 1169.381 kb on - strand, within RR42_RS25960 at 1169.550 kb on + strand, within RR42_RS25960 at 1169.550 kb on + strand, within RR42_RS25960 at 1169.730 kb on - strand, within RR42_RS25960 at 1169.823 kb on + strand, within RR42_RS25960 at 1169.824 kb on - strand, within RR42_RS25960 at 1169.824 kb on - strand, within RR42_RS25960 at 1169.824 kb on - strand, within RR42_RS25960 at 1169.964 kb on + strand, within RR42_RS25960 at 1169.964 kb on + strand, within RR42_RS25960 at 1169.965 kb on - strand, within RR42_RS25960 at 1169.965 kb on - strand, within RR42_RS25960 at 1169.965 kb on - strand, within RR42_RS25960 at 1170.361 kb on + strand at 1170.361 kb on + strand at 1170.362 kb on - strand at 1170.432 kb on + strand at 1170.432 kb on + strand at 1170.433 kb on - strand at 1170.433 kb on - strand at 1170.648 kb on - strand at 1170.648 kb on - strand at 1170.658 kb on + strand at 1170.658 kb on + strand at 1170.659 kb on - strand at 1170.659 kb on - strand at 1170.659 kb on - strand at 1170.718 kb on + strand at 1170.721 kb on + strand at 1170.722 kb on - strand at 1170.759 kb on + strand at 1170.759 kb on + strand at 1170.759 kb on + strand at 1170.786 kb on + strand at 1170.787 kb on - strand at 1170.807 kb on + strand at 1170.808 kb on - strand at 1171.209 kb on - strand, within RR42_RS25965 at 1171.209 kb on - strand, within RR42_RS25965 at 1171.209 kb on - strand, within RR42_RS25965 at 1171.217 kb on - strand, within RR42_RS25965 at 1171.217 kb on - strand, within RR42_RS25965 at 1171.592 kb on - strand at 1171.605 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,165,312 - RR42_RS25950 0.84 -0.0 1,165,332 + RR42_RS25950 0.85 +1.0 1,165,332 + RR42_RS25950 0.85 -0.3 1,165,353 + RR42_RS25950 0.87 +2.0 1,165,354 - RR42_RS25950 0.87 +0.2 1,165,354 - RR42_RS25950 0.87 +0.7 1,165,354 - RR42_RS25950 0.87 +0.2 1,165,937 - -0.4 1,165,937 - +0.0 1,166,011 - -0.6 1,166,038 + -0.3 1,166,038 + +0.1 1,166,039 - +0.0 1,166,039 - -0.0 1,166,047 - -0.4 1,166,047 - +0.0 1,166,190 - +0.9 1,166,249 + -1.3 1,166,251 + -0.4 1,166,251 + -1.2 1,166,252 - +0.9 1,166,252 - -0.3 1,166,252 - -0.1 1,166,319 + +0.0 1,166,320 - -0.9 1,166,820 + RR42_RS25960 0.12 +0.6 1,166,821 - RR42_RS25960 0.12 +0.3 1,166,821 - RR42_RS25960 0.12 +0.1 1,166,880 + RR42_RS25960 0.13 +0.8 1,166,902 - RR42_RS25960 0.14 -0.2 1,167,363 + RR42_RS25960 0.25 +0.5 1,167,423 + RR42_RS25960 0.26 +0.7 1,167,423 + RR42_RS25960 0.26 +0.3 1,167,423 + RR42_RS25960 0.26 -0.4 1,167,423 + RR42_RS25960 0.26 +0.5 1,167,424 - RR42_RS25960 0.26 +1.0 1,167,424 - RR42_RS25960 0.26 -0.2 1,168,029 - RR42_RS25960 0.40 -0.0 1,168,176 + RR42_RS25960 0.43 -0.0 1,168,176 + RR42_RS25960 0.43 -0.3 1,168,177 - RR42_RS25960 0.43 -1.2 1,168,230 + RR42_RS25960 0.45 -0.7 1,168,301 + RR42_RS25960 0.46 +0.1 1,168,301 + RR42_RS25960 0.46 +0.5 1,168,301 + RR42_RS25960 0.46 +0.7 1,168,301 + RR42_RS25960 0.46 +0.2 1,168,301 + RR42_RS25960 0.46 -0.0 1,168,301 + RR42_RS25960 0.46 -2.4 1,168,301 + RR42_RS25960 0.46 +0.7 1,168,302 - RR42_RS25960 0.46 -0.8 1,168,302 - RR42_RS25960 0.46 -0.7 1,168,302 - RR42_RS25960 0.46 -0.2 1,168,302 - RR42_RS25960 0.46 -0.3 1,168,416 + RR42_RS25960 0.49 +0.1 1,168,417 - RR42_RS25960 0.49 -1.5 1,168,417 - RR42_RS25960 0.49 -0.3 1,168,417 - RR42_RS25960 0.49 -0.9 1,168,538 + RR42_RS25960 0.52 -2.0 1,168,560 + RR42_RS25960 0.52 +1.1 1,168,560 + RR42_RS25960 0.52 -0.3 1,168,560 + RR42_RS25960 0.52 +0.9 1,168,561 - RR42_RS25960 0.52 -0.7 1,168,561 - RR42_RS25960 0.52 +0.2 1,168,684 - RR42_RS25960 0.55 -0.7 1,168,768 + RR42_RS25960 0.57 +0.8 1,168,769 - RR42_RS25960 0.57 +0.3 1,168,783 - RR42_RS25960 0.58 -0.7 1,168,803 + RR42_RS25960 0.58 -0.9 1,169,097 + RR42_RS25960 0.65 -1.1 1,169,098 - RR42_RS25960 0.65 -0.1 1,169,154 + RR42_RS25960 0.66 -0.0 1,169,155 - RR42_RS25960 0.66 -0.9 1,169,306 + RR42_RS25960 0.70 -0.5 1,169,310 + RR42_RS25960 0.70 +0.6 1,169,310 + RR42_RS25960 0.70 +0.1 1,169,310 + RR42_RS25960 0.70 -0.2 1,169,310 + RR42_RS25960 0.70 +0.5 1,169,310 + RR42_RS25960 0.70 +1.4 1,169,310 + RR42_RS25960 0.70 +0.8 1,169,311 - RR42_RS25960 0.70 -0.8 1,169,311 - RR42_RS25960 0.70 +1.2 1,169,311 - RR42_RS25960 0.70 -0.0 1,169,311 - RR42_RS25960 0.70 -1.4 1,169,312 + RR42_RS25960 0.70 -0.6 1,169,312 + RR42_RS25960 0.70 -0.6 1,169,380 + RR42_RS25960 0.71 +0.6 1,169,381 - RR42_RS25960 0.71 +0.8 1,169,381 - RR42_RS25960 0.71 +0.2 1,169,550 + RR42_RS25960 0.75 +1.1 1,169,550 + RR42_RS25960 0.75 +1.0 1,169,730 - RR42_RS25960 0.79 -0.2 1,169,823 + RR42_RS25960 0.82 +0.6 1,169,824 - RR42_RS25960 0.82 -0.5 1,169,824 - RR42_RS25960 0.82 +0.7 1,169,824 - RR42_RS25960 0.82 +0.1 1,169,964 + RR42_RS25960 0.85 +0.5 1,169,964 + RR42_RS25960 0.85 -0.5 1,169,965 - RR42_RS25960 0.85 -0.6 1,169,965 - RR42_RS25960 0.85 -1.5 1,169,965 - RR42_RS25960 0.85 -1.0 1,170,361 + +0.1 1,170,361 + +0.5 1,170,362 - +0.5 1,170,432 + +1.0 1,170,432 + -0.4 1,170,433 - +0.7 1,170,433 - -0.4 1,170,648 - +0.2 1,170,648 - +1.0 1,170,658 + +0.1 1,170,658 + +1.1 1,170,659 - +0.4 1,170,659 - +0.0 1,170,659 - +0.1 1,170,718 + -0.6 1,170,721 + +0.2 1,170,722 - -0.6 1,170,759 + -0.1 1,170,759 + +0.5 1,170,759 + -0.4 1,170,786 + +0.6 1,170,787 - -0.6 1,170,807 + +0.1 1,170,808 - -0.2 1,171,209 - RR42_RS25965 0.44 -1.3 1,171,209 - RR42_RS25965 0.44 -1.0 1,171,209 - RR42_RS25965 0.44 +0.5 1,171,217 - RR42_RS25965 0.45 -1.7 1,171,217 - RR42_RS25965 0.45 -0.3 1,171,592 - +0.4 1,171,605 + +1.2
Or see this region's nucleotide sequence