Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25930 and RR42_RS25935 are separated by 17 nucleotides RR42_RS25935 and RR42_RS25940 are separated by 367 nucleotides
RR42_RS25930: RR42_RS25930 - glycine dehydrogenase (deaminating) (EC 1.4.1.10) (from data) , at 1,157,457 to 1,158,725
_RS25930
RR42_RS25935: RR42_RS25935 - mechanosensitive ion channel protein, at 1,158,743 to 1,161,238
_RS25935
RR42_RS25940: RR42_RS25940 - AraC family transcriptional regulator, at 1,161,606 to 1,162,523
_RS25940
Position (kb)
1158
1159
1160
1161
1162 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1157.870 kb on + strand, within RR42_RS25930 at 1157.870 kb on + strand, within RR42_RS25930 at 1157.874 kb on + strand, within RR42_RS25930 at 1157.874 kb on + strand, within RR42_RS25930 at 1158.000 kb on + strand, within RR42_RS25930 at 1158.003 kb on + strand, within RR42_RS25930 at 1158.003 kb on + strand, within RR42_RS25930 at 1158.003 kb on + strand, within RR42_RS25930 at 1158.003 kb on + strand, within RR42_RS25930 at 1158.013 kb on - strand, within RR42_RS25930 at 1158.013 kb on - strand, within RR42_RS25930 at 1158.103 kb on - strand, within RR42_RS25930 at 1158.264 kb on + strand, within RR42_RS25930 at 1158.264 kb on + strand, within RR42_RS25930 at 1158.264 kb on + strand, within RR42_RS25930 at 1158.803 kb on - strand at 1158.878 kb on + strand at 1158.878 kb on + strand at 1158.882 kb on + strand at 1158.882 kb on + strand at 1159.375 kb on - strand, within RR42_RS25935 at 1159.375 kb on - strand, within RR42_RS25935 at 1159.798 kb on - strand, within RR42_RS25935 at 1159.837 kb on - strand, within RR42_RS25935 at 1159.863 kb on + strand, within RR42_RS25935 at 1159.863 kb on + strand, within RR42_RS25935 at 1159.863 kb on + strand, within RR42_RS25935 at 1159.864 kb on - strand, within RR42_RS25935 at 1160.021 kb on + strand, within RR42_RS25935 at 1160.037 kb on - strand, within RR42_RS25935 at 1160.051 kb on + strand, within RR42_RS25935 at 1160.052 kb on - strand, within RR42_RS25935 at 1160.052 kb on - strand, within RR42_RS25935 at 1161.122 kb on + strand at 1161.123 kb on - strand at 1161.123 kb on - strand at 1161.355 kb on + strand at 1161.356 kb on - strand at 1161.357 kb on + strand at 1161.357 kb on + strand at 1161.357 kb on + strand at 1161.358 kb on - strand at 1161.436 kb on - strand at 1161.436 kb on - strand at 1161.436 kb on - strand at 1161.436 kb on - strand at 1161.436 kb on - strand at 1161.436 kb on - strand at 1161.438 kb on - strand at 1161.590 kb on - strand at 1161.764 kb on + strand, within RR42_RS25940 at 1161.764 kb on + strand, within RR42_RS25940 at 1161.872 kb on + strand, within RR42_RS25940 at 1161.872 kb on + strand, within RR42_RS25940 at 1161.872 kb on + strand, within RR42_RS25940 at 1161.882 kb on + strand, within RR42_RS25940 at 1161.882 kb on + strand, within RR42_RS25940 at 1161.882 kb on + strand, within RR42_RS25940 at 1161.882 kb on + strand, within RR42_RS25940 at 1161.882 kb on + strand, within RR42_RS25940 at 1161.882 kb on + strand, within RR42_RS25940 at 1161.883 kb on - strand, within RR42_RS25940 at 1161.908 kb on + strand, within RR42_RS25940 at 1161.908 kb on + strand, within RR42_RS25940 at 1161.909 kb on - strand, within RR42_RS25940 at 1161.988 kb on + strand, within RR42_RS25940 at 1161.988 kb on + strand, within RR42_RS25940 at 1161.989 kb on - strand, within RR42_RS25940 at 1162.141 kb on - strand, within RR42_RS25940 at 1162.141 kb on - strand, within RR42_RS25940 at 1162.141 kb on - strand, within RR42_RS25940
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,157,870 + RR42_RS25930 0.33 -0.5 1,157,870 + RR42_RS25930 0.33 +0.4 1,157,874 + RR42_RS25930 0.33 -0.4 1,157,874 + RR42_RS25930 0.33 +0.4 1,158,000 + RR42_RS25930 0.43 -0.6 1,158,003 + RR42_RS25930 0.43 -0.6 1,158,003 + RR42_RS25930 0.43 +1.0 1,158,003 + RR42_RS25930 0.43 +0.6 1,158,003 + RR42_RS25930 0.43 +0.5 1,158,013 - RR42_RS25930 0.44 +0.0 1,158,013 - RR42_RS25930 0.44 +0.0 1,158,103 - RR42_RS25930 0.51 -0.0 1,158,264 + RR42_RS25930 0.64 +1.3 1,158,264 + RR42_RS25930 0.64 +0.1 1,158,264 + RR42_RS25930 0.64 -1.0 1,158,803 - +0.8 1,158,878 + -1.1 1,158,878 + +1.0 1,158,882 + +0.0 1,158,882 + +0.3 1,159,375 - RR42_RS25935 0.25 -0.0 1,159,375 - RR42_RS25935 0.25 -0.1 1,159,798 - RR42_RS25935 0.42 -1.0 1,159,837 - RR42_RS25935 0.44 -0.3 1,159,863 + RR42_RS25935 0.45 -0.9 1,159,863 + RR42_RS25935 0.45 -0.0 1,159,863 + RR42_RS25935 0.45 -0.1 1,159,864 - RR42_RS25935 0.45 +1.0 1,160,021 + RR42_RS25935 0.51 -0.0 1,160,037 - RR42_RS25935 0.52 +0.2 1,160,051 + RR42_RS25935 0.52 +0.2 1,160,052 - RR42_RS25935 0.52 +1.0 1,160,052 - RR42_RS25935 0.52 -2.0 1,161,122 + -0.5 1,161,123 - +0.1 1,161,123 - +0.4 1,161,355 + -1.6 1,161,356 - +0.6 1,161,357 + -0.2 1,161,357 + -0.2 1,161,357 + +0.0 1,161,358 - -0.6 1,161,436 - -0.6 1,161,436 - +0.7 1,161,436 - +1.0 1,161,436 - +0.2 1,161,436 - -0.2 1,161,436 - -1.8 1,161,438 - -0.1 1,161,590 - -1.2 1,161,764 + RR42_RS25940 0.17 -0.6 1,161,764 + RR42_RS25940 0.17 +1.3 1,161,872 + RR42_RS25940 0.29 -0.5 1,161,872 + RR42_RS25940 0.29 -0.0 1,161,872 + RR42_RS25940 0.29 +2.2 1,161,882 + RR42_RS25940 0.30 -0.9 1,161,882 + RR42_RS25940 0.30 -1.0 1,161,882 + RR42_RS25940 0.30 -0.0 1,161,882 + RR42_RS25940 0.30 +0.2 1,161,882 + RR42_RS25940 0.30 +0.5 1,161,882 + RR42_RS25940 0.30 -0.0 1,161,883 - RR42_RS25940 0.30 -0.4 1,161,908 + RR42_RS25940 0.33 -0.4 1,161,908 + RR42_RS25940 0.33 -0.9 1,161,909 - RR42_RS25940 0.33 -1.2 1,161,988 + RR42_RS25940 0.42 +0.1 1,161,988 + RR42_RS25940 0.42 +0.2 1,161,989 - RR42_RS25940 0.42 +2.7 1,162,141 - RR42_RS25940 0.58 +0.3 1,162,141 - RR42_RS25940 0.58 -0.3 1,162,141 - RR42_RS25940 0.58 -0.0
Or see this region's nucleotide sequence