Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25740 and RR42_RS25745 are separated by 299 nucleotides RR42_RS25745 and RR42_RS25750 are separated by 155 nucleotides RR42_RS25750 and RR42_RS25755 are separated by 25 nucleotides RR42_RS25755 and RR42_RS25760 are separated by 48 nucleotides
RR42_RS25740: RR42_RS25740 - hypothetical protein, at 1,116,463 to 1,117,206
_RS25740
RR42_RS25745: RR42_RS25745 - tRNA-Thr, at 1,117,506 to 1,117,580
_RS25745
RR42_RS25750: RR42_RS25750 - aromatic ring-cleaving dioxygenase, at 1,117,736 to 1,118,557
_RS25750
RR42_RS25755: RR42_RS25755 - IclR family transcriptional regulator, at 1,118,583 to 1,119,440
_RS25755
RR42_RS25760: RR42_RS25760 - salicylate hydroxylase, at 1,119,489 to 1,120,619
_RS25760
Position (kb)
1117
1118
1119 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1116.837 kb on - strand, within RR42_RS25740 at 1116.848 kb on + strand, within RR42_RS25740 at 1116.849 kb on - strand, within RR42_RS25740 at 1116.849 kb on - strand, within RR42_RS25740 at 1117.235 kb on - strand at 1117.283 kb on + strand at 1117.390 kb on + strand at 1117.479 kb on - strand at 1117.483 kb on + strand at 1117.483 kb on + strand at 1117.634 kb on + strand at 1117.634 kb on + strand at 1117.635 kb on - strand at 1117.635 kb on - strand at 1117.635 kb on - strand at 1117.753 kb on + strand at 1117.753 kb on + strand at 1117.753 kb on + strand at 1117.753 kb on + strand at 1117.754 kb on - strand at 1117.754 kb on - strand at 1117.754 kb on - strand at 1118.022 kb on + strand, within RR42_RS25750 at 1118.022 kb on + strand, within RR42_RS25750 at 1118.023 kb on - strand, within RR42_RS25750 at 1118.157 kb on + strand, within RR42_RS25750 at 1118.157 kb on + strand, within RR42_RS25750 at 1118.157 kb on + strand, within RR42_RS25750 at 1118.158 kb on - strand, within RR42_RS25750 at 1118.286 kb on + strand, within RR42_RS25750 at 1118.287 kb on - strand, within RR42_RS25750 at 1118.298 kb on + strand, within RR42_RS25750 at 1118.304 kb on + strand, within RR42_RS25750 at 1118.304 kb on + strand, within RR42_RS25750 at 1118.305 kb on - strand, within RR42_RS25750 at 1118.319 kb on + strand, within RR42_RS25750 at 1118.322 kb on + strand, within RR42_RS25750 at 1118.323 kb on - strand, within RR42_RS25750 at 1118.323 kb on - strand, within RR42_RS25750 at 1118.323 kb on - strand, within RR42_RS25750 at 1118.328 kb on + strand, within RR42_RS25750 at 1118.328 kb on + strand, within RR42_RS25750 at 1118.328 kb on + strand, within RR42_RS25750 at 1118.329 kb on - strand, within RR42_RS25750 at 1118.329 kb on - strand, within RR42_RS25750 at 1118.473 kb on - strand, within RR42_RS25750 at 1118.852 kb on - strand, within RR42_RS25755 at 1118.991 kb on - strand, within RR42_RS25755 at 1119.031 kb on + strand, within RR42_RS25755 at 1119.064 kb on + strand, within RR42_RS25755 at 1119.501 kb on + strand at 1119.502 kb on - strand at 1119.502 kb on - strand at 1119.517 kb on + strand at 1119.518 kb on - strand at 1119.518 kb on - strand at 1119.518 kb on - strand at 1119.518 kb on - strand at 1119.523 kb on + strand at 1119.523 kb on + strand at 1119.524 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,116,837 - RR42_RS25740 0.50 +0.2 1,116,848 + RR42_RS25740 0.52 -0.0 1,116,849 - RR42_RS25740 0.52 -1.1 1,116,849 - RR42_RS25740 0.52 -0.3 1,117,235 - -0.2 1,117,283 + -0.0 1,117,390 + -0.2 1,117,479 - -0.2 1,117,483 + -0.0 1,117,483 + -1.7 1,117,634 + -2.2 1,117,634 + +0.1 1,117,635 - +1.7 1,117,635 - -0.2 1,117,635 - +0.0 1,117,753 + +0.5 1,117,753 + -0.0 1,117,753 + -0.6 1,117,753 + +0.1 1,117,754 - -0.6 1,117,754 - -0.6 1,117,754 - +1.5 1,118,022 + RR42_RS25750 0.35 -0.1 1,118,022 + RR42_RS25750 0.35 -0.7 1,118,023 - RR42_RS25750 0.35 -0.5 1,118,157 + RR42_RS25750 0.51 +0.2 1,118,157 + RR42_RS25750 0.51 -0.6 1,118,157 + RR42_RS25750 0.51 +1.4 1,118,158 - RR42_RS25750 0.51 -0.0 1,118,286 + RR42_RS25750 0.67 -0.6 1,118,287 - RR42_RS25750 0.67 -0.7 1,118,298 + RR42_RS25750 0.68 +0.3 1,118,304 + RR42_RS25750 0.69 -0.4 1,118,304 + RR42_RS25750 0.69 -1.6 1,118,305 - RR42_RS25750 0.69 +0.1 1,118,319 + RR42_RS25750 0.71 +0.1 1,118,322 + RR42_RS25750 0.71 -0.9 1,118,323 - RR42_RS25750 0.71 -0.0 1,118,323 - RR42_RS25750 0.71 -0.3 1,118,323 - RR42_RS25750 0.71 +0.4 1,118,328 + RR42_RS25750 0.72 +0.5 1,118,328 + RR42_RS25750 0.72 -0.3 1,118,328 + RR42_RS25750 0.72 -0.5 1,118,329 - RR42_RS25750 0.72 -0.5 1,118,329 - RR42_RS25750 0.72 +0.1 1,118,473 - RR42_RS25750 0.90 +2.4 1,118,852 - RR42_RS25755 0.31 -1.5 1,118,991 - RR42_RS25755 0.48 -0.3 1,119,031 + RR42_RS25755 0.52 -3.0 1,119,064 + RR42_RS25755 0.56 -1.2 1,119,501 + +0.4 1,119,502 - -0.7 1,119,502 - -0.0 1,119,517 + -1.4 1,119,518 - -1.7 1,119,518 - +0.2 1,119,518 - +0.7 1,119,518 - +0.3 1,119,523 + +0.1 1,119,523 + -1.3 1,119,524 - +0.0
Or see this region's nucleotide sequence