Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25750

Experiment: R2A with Sisomicin 0.035 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25740 and RR42_RS25745 are separated by 299 nucleotidesRR42_RS25745 and RR42_RS25750 are separated by 155 nucleotidesRR42_RS25750 and RR42_RS25755 are separated by 25 nucleotidesRR42_RS25755 and RR42_RS25760 are separated by 48 nucleotides RR42_RS25740: RR42_RS25740 - hypothetical protein, at 1,116,463 to 1,117,206 _RS25740 RR42_RS25745: RR42_RS25745 - tRNA-Thr, at 1,117,506 to 1,117,580 _RS25745 RR42_RS25750: RR42_RS25750 - aromatic ring-cleaving dioxygenase, at 1,117,736 to 1,118,557 _RS25750 RR42_RS25755: RR42_RS25755 - IclR family transcriptional regulator, at 1,118,583 to 1,119,440 _RS25755 RR42_RS25760: RR42_RS25760 - salicylate hydroxylase, at 1,119,489 to 1,120,619 _RS25760 Position (kb) 1117 1118 1119Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1116.837 kb on - strand, within RR42_RS25740at 1116.848 kb on + strand, within RR42_RS25740at 1116.849 kb on - strand, within RR42_RS25740at 1116.849 kb on - strand, within RR42_RS25740at 1117.235 kb on - strandat 1117.283 kb on + strandat 1117.390 kb on + strandat 1117.479 kb on - strandat 1117.483 kb on + strandat 1117.483 kb on + strandat 1117.634 kb on + strandat 1117.634 kb on + strandat 1117.635 kb on - strandat 1117.635 kb on - strandat 1117.635 kb on - strandat 1117.753 kb on + strandat 1117.753 kb on + strandat 1117.753 kb on + strandat 1117.753 kb on + strandat 1117.754 kb on - strandat 1117.754 kb on - strandat 1117.754 kb on - strandat 1118.022 kb on + strand, within RR42_RS25750at 1118.022 kb on + strand, within RR42_RS25750at 1118.023 kb on - strand, within RR42_RS25750at 1118.157 kb on + strand, within RR42_RS25750at 1118.157 kb on + strand, within RR42_RS25750at 1118.157 kb on + strand, within RR42_RS25750at 1118.158 kb on - strand, within RR42_RS25750at 1118.286 kb on + strand, within RR42_RS25750at 1118.287 kb on - strand, within RR42_RS25750at 1118.298 kb on + strand, within RR42_RS25750at 1118.304 kb on + strand, within RR42_RS25750at 1118.304 kb on + strand, within RR42_RS25750at 1118.305 kb on - strand, within RR42_RS25750at 1118.319 kb on + strand, within RR42_RS25750at 1118.322 kb on + strand, within RR42_RS25750at 1118.323 kb on - strand, within RR42_RS25750at 1118.323 kb on - strand, within RR42_RS25750at 1118.323 kb on - strand, within RR42_RS25750at 1118.328 kb on + strand, within RR42_RS25750at 1118.328 kb on + strand, within RR42_RS25750at 1118.328 kb on + strand, within RR42_RS25750at 1118.329 kb on - strand, within RR42_RS25750at 1118.329 kb on - strand, within RR42_RS25750at 1118.473 kb on - strand, within RR42_RS25750at 1118.852 kb on - strand, within RR42_RS25755at 1118.991 kb on - strand, within RR42_RS25755at 1119.031 kb on + strand, within RR42_RS25755at 1119.064 kb on + strand, within RR42_RS25755at 1119.501 kb on + strandat 1119.502 kb on - strandat 1119.502 kb on - strandat 1119.517 kb on + strandat 1119.518 kb on - strandat 1119.518 kb on - strandat 1119.518 kb on - strandat 1119.518 kb on - strandat 1119.523 kb on + strandat 1119.523 kb on + strandat 1119.524 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml
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1,116,837 - RR42_RS25740 0.50 +0.2
1,116,848 + RR42_RS25740 0.52 -0.0
1,116,849 - RR42_RS25740 0.52 -1.1
1,116,849 - RR42_RS25740 0.52 -0.3
1,117,235 - -0.2
1,117,283 + -0.0
1,117,390 + -0.2
1,117,479 - -0.2
1,117,483 + -0.0
1,117,483 + -1.7
1,117,634 + -2.2
1,117,634 + +0.1
1,117,635 - +1.7
1,117,635 - -0.2
1,117,635 - +0.0
1,117,753 + +0.5
1,117,753 + -0.0
1,117,753 + -0.6
1,117,753 + +0.1
1,117,754 - -0.6
1,117,754 - -0.6
1,117,754 - +1.5
1,118,022 + RR42_RS25750 0.35 -0.1
1,118,022 + RR42_RS25750 0.35 -0.7
1,118,023 - RR42_RS25750 0.35 -0.5
1,118,157 + RR42_RS25750 0.51 +0.2
1,118,157 + RR42_RS25750 0.51 -0.6
1,118,157 + RR42_RS25750 0.51 +1.4
1,118,158 - RR42_RS25750 0.51 -0.0
1,118,286 + RR42_RS25750 0.67 -0.6
1,118,287 - RR42_RS25750 0.67 -0.7
1,118,298 + RR42_RS25750 0.68 +0.3
1,118,304 + RR42_RS25750 0.69 -0.4
1,118,304 + RR42_RS25750 0.69 -1.6
1,118,305 - RR42_RS25750 0.69 +0.1
1,118,319 + RR42_RS25750 0.71 +0.1
1,118,322 + RR42_RS25750 0.71 -0.9
1,118,323 - RR42_RS25750 0.71 -0.0
1,118,323 - RR42_RS25750 0.71 -0.3
1,118,323 - RR42_RS25750 0.71 +0.4
1,118,328 + RR42_RS25750 0.72 +0.5
1,118,328 + RR42_RS25750 0.72 -0.3
1,118,328 + RR42_RS25750 0.72 -0.5
1,118,329 - RR42_RS25750 0.72 -0.5
1,118,329 - RR42_RS25750 0.72 +0.1
1,118,473 - RR42_RS25750 0.90 +2.4
1,118,852 - RR42_RS25755 0.31 -1.5
1,118,991 - RR42_RS25755 0.48 -0.3
1,119,031 + RR42_RS25755 0.52 -3.0
1,119,064 + RR42_RS25755 0.56 -1.2
1,119,501 + +0.4
1,119,502 - -0.7
1,119,502 - -0.0
1,119,517 + -1.4
1,119,518 - -1.7
1,119,518 - +0.2
1,119,518 - +0.7
1,119,518 - +0.3
1,119,523 + +0.1
1,119,523 + -1.3
1,119,524 - +0.0

Or see this region's nucleotide sequence