Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25640 and RR42_RS25645 are separated by 115 nucleotides RR42_RS25645 and RR42_RS25650 are separated by 316 nucleotides RR42_RS25650 and RR42_RS25655 are separated by 95 nucleotides RR42_RS25655 and RR42_RS25660 are separated by 8 nucleotides
RR42_RS25640: RR42_RS25640 - 3-hydroxyisobutyrate dehydrogenase, at 1,096,083 to 1,096,979
_RS25640
RR42_RS25645: RR42_RS25645 - hypothetical protein, at 1,097,095 to 1,097,289
_RS25645
RR42_RS25650: RR42_RS25650 - hypothetical protein, at 1,097,606 to 1,097,806
_RS25650
RR42_RS25655: RR42_RS25655 - pyruvate carboxylase, at 1,097,902 to 1,101,411
_RS25655
RR42_RS25660: RR42_RS25660 - cytochrome C biogenesis protein, at 1,101,420 to 1,102,091
_RS25660
Position (kb)
1097
1098
1099
1100
1101
1102 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1097.089 kb on + strand at 1097.089 kb on + strand at 1097.089 kb on + strand at 1097.097 kb on - strand at 1097.250 kb on + strand, within RR42_RS25645 at 1097.250 kb on + strand, within RR42_RS25645 at 1097.251 kb on - strand, within RR42_RS25645 at 1097.251 kb on - strand, within RR42_RS25645 at 1097.294 kb on + strand at 1097.295 kb on - strand at 1097.295 kb on - strand at 1097.295 kb on - strand at 1097.338 kb on + strand at 1097.338 kb on + strand at 1097.338 kb on + strand at 1097.339 kb on - strand at 1097.339 kb on - strand at 1097.460 kb on - strand at 1097.610 kb on - strand at 1097.610 kb on - strand at 1097.610 kb on - strand at 1097.613 kb on - strand at 1097.613 kb on - strand at 1097.704 kb on + strand, within RR42_RS25650 at 1097.704 kb on + strand, within RR42_RS25650 at 1097.798 kb on + strand at 1097.799 kb on - strand at 1097.799 kb on - strand at 1097.901 kb on + strand at 1097.908 kb on + strand at 1097.908 kb on + strand at 1097.989 kb on - strand at 1098.000 kb on - strand at 1098.007 kb on + strand at 1098.059 kb on - strand at 1098.095 kb on - strand at 1098.508 kb on + strand, within RR42_RS25655 at 1098.508 kb on + strand, within RR42_RS25655 at 1098.508 kb on + strand, within RR42_RS25655 at 1098.509 kb on - strand, within RR42_RS25655 at 1098.509 kb on - strand, within RR42_RS25655 at 1098.589 kb on + strand, within RR42_RS25655 at 1098.589 kb on + strand, within RR42_RS25655 at 1098.590 kb on - strand, within RR42_RS25655 at 1098.590 kb on - strand, within RR42_RS25655 at 1098.603 kb on + strand, within RR42_RS25655 at 1098.604 kb on - strand, within RR42_RS25655 at 1098.604 kb on - strand, within RR42_RS25655 at 1098.772 kb on + strand, within RR42_RS25655 at 1098.772 kb on + strand, within RR42_RS25655 at 1098.773 kb on - strand, within RR42_RS25655 at 1098.810 kb on + strand, within RR42_RS25655 at 1098.811 kb on - strand, within RR42_RS25655 at 1098.811 kb on - strand, within RR42_RS25655 at 1099.030 kb on + strand, within RR42_RS25655 at 1099.123 kb on + strand, within RR42_RS25655 at 1099.366 kb on + strand, within RR42_RS25655 at 1099.624 kb on - strand, within RR42_RS25655 at 1099.825 kb on + strand, within RR42_RS25655 at 1099.825 kb on + strand, within RR42_RS25655 at 1099.826 kb on - strand, within RR42_RS25655 at 1100.018 kb on - strand, within RR42_RS25655 at 1100.018 kb on - strand, within RR42_RS25655 at 1100.029 kb on + strand, within RR42_RS25655 at 1100.029 kb on + strand, within RR42_RS25655 at 1100.030 kb on - strand, within RR42_RS25655 at 1100.030 kb on - strand, within RR42_RS25655 at 1100.032 kb on + strand, within RR42_RS25655 at 1100.033 kb on - strand, within RR42_RS25655 at 1100.287 kb on - strand, within RR42_RS25655 at 1100.372 kb on - strand, within RR42_RS25655 at 1100.372 kb on - strand, within RR42_RS25655 at 1100.372 kb on - strand, within RR42_RS25655 at 1100.372 kb on - strand, within RR42_RS25655 at 1100.384 kb on + strand, within RR42_RS25655 at 1100.393 kb on - strand, within RR42_RS25655 at 1100.395 kb on + strand, within RR42_RS25655 at 1100.395 kb on + strand, within RR42_RS25655 at 1100.536 kb on + strand, within RR42_RS25655 at 1100.537 kb on - strand, within RR42_RS25655 at 1100.894 kb on - strand, within RR42_RS25655 at 1100.936 kb on - strand, within RR42_RS25655 at 1100.938 kb on - strand, within RR42_RS25655 at 1100.938 kb on - strand, within RR42_RS25655 at 1100.943 kb on + strand, within RR42_RS25655 at 1101.352 kb on - strand at 1101.456 kb on + strand at 1101.457 kb on - strand at 1101.642 kb on - strand, within RR42_RS25660 at 1101.663 kb on + strand, within RR42_RS25660 at 1101.664 kb on - strand, within RR42_RS25660 at 1101.673 kb on - strand, within RR42_RS25660 at 1101.732 kb on + strand, within RR42_RS25660 at 1101.994 kb on - strand, within RR42_RS25660 at 1102.072 kb on - strand at 1102.143 kb on + strand at 1102.144 kb on - strand at 1102.388 kb on + strand at 1102.389 kb on - strand at 1102.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,097,089 + -2.3 1,097,089 + -2.2 1,097,089 + -1.4 1,097,097 - -0.6 1,097,250 + RR42_RS25645 0.79 -0.8 1,097,250 + RR42_RS25645 0.79 -0.6 1,097,251 - RR42_RS25645 0.80 -0.5 1,097,251 - RR42_RS25645 0.80 -0.4 1,097,294 + -0.3 1,097,295 - -0.6 1,097,295 - -1.0 1,097,295 - +0.2 1,097,338 + -0.0 1,097,338 + +0.1 1,097,338 + -0.0 1,097,339 - +0.0 1,097,339 - -0.2 1,097,460 - +0.3 1,097,610 - -0.6 1,097,610 - +0.2 1,097,610 - -0.7 1,097,613 - -0.3 1,097,613 - -0.4 1,097,704 + RR42_RS25650 0.49 -2.6 1,097,704 + RR42_RS25650 0.49 +0.2 1,097,798 + -0.4 1,097,799 - -2.7 1,097,799 - +0.3 1,097,901 + -0.2 1,097,908 + +0.8 1,097,908 + +0.0 1,097,989 - +0.7 1,098,000 - -0.9 1,098,007 + +1.0 1,098,059 - +0.1 1,098,095 - +0.1 1,098,508 + RR42_RS25655 0.17 -1.0 1,098,508 + RR42_RS25655 0.17 +1.0 1,098,508 + RR42_RS25655 0.17 -1.4 1,098,509 - RR42_RS25655 0.17 +0.5 1,098,509 - RR42_RS25655 0.17 +0.6 1,098,589 + RR42_RS25655 0.20 -0.3 1,098,589 + RR42_RS25655 0.20 +1.8 1,098,590 - RR42_RS25655 0.20 +0.7 1,098,590 - RR42_RS25655 0.20 +0.3 1,098,603 + RR42_RS25655 0.20 +1.2 1,098,604 - RR42_RS25655 0.20 +2.0 1,098,604 - RR42_RS25655 0.20 -0.6 1,098,772 + RR42_RS25655 0.25 -0.3 1,098,772 + RR42_RS25655 0.25 -0.2 1,098,773 - RR42_RS25655 0.25 +0.4 1,098,810 + RR42_RS25655 0.26 +0.4 1,098,811 - RR42_RS25655 0.26 +0.0 1,098,811 - RR42_RS25655 0.26 -0.0 1,099,030 + RR42_RS25655 0.32 -0.6 1,099,123 + RR42_RS25655 0.35 -0.2 1,099,366 + RR42_RS25655 0.42 +1.7 1,099,624 - RR42_RS25655 0.49 -0.4 1,099,825 + RR42_RS25655 0.55 -0.3 1,099,825 + RR42_RS25655 0.55 +0.2 1,099,826 - RR42_RS25655 0.55 +0.1 1,100,018 - RR42_RS25655 0.60 -0.1 1,100,018 - RR42_RS25655 0.60 -1.0 1,100,029 + RR42_RS25655 0.61 -0.6 1,100,029 + RR42_RS25655 0.61 +0.7 1,100,030 - RR42_RS25655 0.61 +0.1 1,100,030 - RR42_RS25655 0.61 +2.5 1,100,032 + RR42_RS25655 0.61 +0.4 1,100,033 - RR42_RS25655 0.61 +0.5 1,100,287 - RR42_RS25655 0.68 -0.5 1,100,372 - RR42_RS25655 0.70 +0.1 1,100,372 - RR42_RS25655 0.70 +0.2 1,100,372 - RR42_RS25655 0.70 +0.1 1,100,372 - RR42_RS25655 0.70 -0.2 1,100,384 + RR42_RS25655 0.71 +0.3 1,100,393 - RR42_RS25655 0.71 +1.0 1,100,395 + RR42_RS25655 0.71 +2.0 1,100,395 + RR42_RS25655 0.71 -1.4 1,100,536 + RR42_RS25655 0.75 -2.5 1,100,537 - RR42_RS25655 0.75 -0.4 1,100,894 - RR42_RS25655 0.85 +0.6 1,100,936 - RR42_RS25655 0.86 -0.1 1,100,938 - RR42_RS25655 0.86 -0.5 1,100,938 - RR42_RS25655 0.86 +1.1 1,100,943 + RR42_RS25655 0.87 +1.5 1,101,352 - +0.2 1,101,456 + +0.0 1,101,457 - -1.5 1,101,642 - RR42_RS25660 0.33 +1.3 1,101,663 + RR42_RS25660 0.36 -0.0 1,101,664 - RR42_RS25660 0.36 +2.4 1,101,673 - RR42_RS25660 0.38 +0.2 1,101,732 + RR42_RS25660 0.46 +1.0 1,101,994 - RR42_RS25660 0.85 +1.0 1,102,072 - +0.1 1,102,143 + -0.2 1,102,144 - +0.3 1,102,388 + +0.5 1,102,389 - +0.8 1,102,389 - -0.3
Or see this region's nucleotide sequence