Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25640 and RR42_RS25645 are separated by 115 nucleotides RR42_RS25645 and RR42_RS25650 are separated by 316 nucleotides RR42_RS25650 and RR42_RS25655 are separated by 95 nucleotides
RR42_RS25640: RR42_RS25640 - 3-hydroxyisobutyrate dehydrogenase, at 1,096,083 to 1,096,979
_RS25640
RR42_RS25645: RR42_RS25645 - hypothetical protein, at 1,097,095 to 1,097,289
_RS25645
RR42_RS25650: RR42_RS25650 - hypothetical protein, at 1,097,606 to 1,097,806
_RS25650
RR42_RS25655: RR42_RS25655 - pyruvate carboxylase, at 1,097,902 to 1,101,411
_RS25655
Position (kb)
1097
1098 Strain fitness (log2 ratio)
-2
-1
0
1 at 1096.302 kb on + strand, within RR42_RS25640 at 1096.302 kb on + strand, within RR42_RS25640 at 1097.089 kb on + strand at 1097.089 kb on + strand at 1097.089 kb on + strand at 1097.097 kb on - strand at 1097.250 kb on + strand, within RR42_RS25645 at 1097.250 kb on + strand, within RR42_RS25645 at 1097.251 kb on - strand, within RR42_RS25645 at 1097.251 kb on - strand, within RR42_RS25645 at 1097.294 kb on + strand at 1097.295 kb on - strand at 1097.295 kb on - strand at 1097.295 kb on - strand at 1097.338 kb on + strand at 1097.338 kb on + strand at 1097.338 kb on + strand at 1097.339 kb on - strand at 1097.339 kb on - strand at 1097.460 kb on - strand at 1097.610 kb on - strand at 1097.610 kb on - strand at 1097.610 kb on - strand at 1097.613 kb on - strand at 1097.613 kb on - strand at 1097.704 kb on + strand, within RR42_RS25650 at 1097.704 kb on + strand, within RR42_RS25650 at 1097.798 kb on + strand at 1097.799 kb on - strand at 1097.799 kb on - strand at 1097.901 kb on + strand at 1097.908 kb on + strand at 1097.908 kb on + strand at 1097.989 kb on - strand at 1098.000 kb on - strand at 1098.007 kb on + strand at 1098.059 kb on - strand at 1098.095 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,096,302 + RR42_RS25640 0.24 -0.8 1,096,302 + RR42_RS25640 0.24 -1.2 1,097,089 + -2.3 1,097,089 + -2.2 1,097,089 + -1.4 1,097,097 - -0.6 1,097,250 + RR42_RS25645 0.79 -0.8 1,097,250 + RR42_RS25645 0.79 -0.6 1,097,251 - RR42_RS25645 0.80 -0.5 1,097,251 - RR42_RS25645 0.80 -0.4 1,097,294 + -0.3 1,097,295 - -0.6 1,097,295 - -1.0 1,097,295 - +0.2 1,097,338 + -0.0 1,097,338 + +0.1 1,097,338 + -0.0 1,097,339 - +0.0 1,097,339 - -0.2 1,097,460 - +0.3 1,097,610 - -0.6 1,097,610 - +0.2 1,097,610 - -0.7 1,097,613 - -0.3 1,097,613 - -0.4 1,097,704 + RR42_RS25650 0.49 -2.6 1,097,704 + RR42_RS25650 0.49 +0.2 1,097,798 + -0.4 1,097,799 - -2.7 1,097,799 - +0.3 1,097,901 + -0.2 1,097,908 + +0.8 1,097,908 + +0.0 1,097,989 - +0.7 1,098,000 - -0.9 1,098,007 + +1.0 1,098,059 - +0.1 1,098,095 - +0.1
Or see this region's nucleotide sequence