Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25265 and RR42_RS25270 are separated by 179 nucleotides RR42_RS25270 and RR42_RS25275 are separated by 241 nucleotides
RR42_RS25265: RR42_RS25265 - hypothetical protein, at 1,008,459 to 1,008,830
_RS25265
RR42_RS25270: RR42_RS25270 - MFS transporter, at 1,009,010 to 1,010,350
_RS25270
RR42_RS25275: RR42_RS25275 - LysR family transcriptional regulator, at 1,010,592 to 1,011,500
_RS25275
Position (kb)
1009
1010
1011 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1008.122 kb on - strand at 1008.951 kb on + strand at 1009.470 kb on - strand, within RR42_RS25270 at 1009.523 kb on + strand, within RR42_RS25270 at 1009.524 kb on - strand, within RR42_RS25270 at 1009.524 kb on - strand, within RR42_RS25270 at 1009.524 kb on - strand, within RR42_RS25270 at 1009.524 kb on - strand, within RR42_RS25270 at 1009.524 kb on - strand, within RR42_RS25270 at 1009.589 kb on + strand, within RR42_RS25270 at 1009.590 kb on - strand, within RR42_RS25270 at 1009.590 kb on - strand, within RR42_RS25270 at 1009.793 kb on + strand, within RR42_RS25270 at 1009.793 kb on + strand, within RR42_RS25270 at 1009.793 kb on + strand, within RR42_RS25270 at 1009.817 kb on + strand, within RR42_RS25270 at 1009.862 kb on + strand, within RR42_RS25270 at 1009.863 kb on - strand, within RR42_RS25270 at 1010.096 kb on + strand, within RR42_RS25270 at 1010.381 kb on + strand at 1010.381 kb on + strand at 1010.382 kb on - strand at 1010.382 kb on - strand at 1010.382 kb on - strand at 1010.485 kb on - strand at 1010.593 kb on + strand at 1010.834 kb on + strand, within RR42_RS25275 at 1010.835 kb on - strand, within RR42_RS25275 at 1010.835 kb on - strand, within RR42_RS25275 at 1010.836 kb on + strand, within RR42_RS25275 at 1010.836 kb on + strand, within RR42_RS25275 at 1010.875 kb on - strand, within RR42_RS25275 at 1010.910 kb on + strand, within RR42_RS25275 at 1010.910 kb on + strand, within RR42_RS25275 at 1010.911 kb on - strand, within RR42_RS25275 at 1010.911 kb on - strand, within RR42_RS25275 at 1010.911 kb on - strand, within RR42_RS25275 at 1010.911 kb on - strand, within RR42_RS25275 at 1010.911 kb on - strand, within RR42_RS25275 at 1010.911 kb on - strand, within RR42_RS25275 at 1010.911 kb on - strand, within RR42_RS25275 at 1010.912 kb on + strand, within RR42_RS25275 at 1010.912 kb on + strand, within RR42_RS25275 at 1010.913 kb on - strand, within RR42_RS25275 at 1011.051 kb on + strand, within RR42_RS25275 at 1011.052 kb on - strand, within RR42_RS25275 at 1011.052 kb on - strand, within RR42_RS25275 at 1011.052 kb on - strand, within RR42_RS25275 at 1011.052 kb on - strand, within RR42_RS25275 at 1011.162 kb on + strand, within RR42_RS25275 at 1011.162 kb on + strand, within RR42_RS25275 at 1011.163 kb on - strand, within RR42_RS25275 at 1011.163 kb on - strand, within RR42_RS25275 at 1011.163 kb on - strand, within RR42_RS25275 at 1011.163 kb on - strand, within RR42_RS25275 at 1011.163 kb on - strand, within RR42_RS25275 at 1011.213 kb on + strand, within RR42_RS25275 at 1011.214 kb on - strand, within RR42_RS25275 at 1011.214 kb on - strand, within RR42_RS25275 at 1011.225 kb on + strand, within RR42_RS25275 at 1011.298 kb on - strand, within RR42_RS25275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,008,122 - -0.2 1,008,951 + +0.4 1,009,470 - RR42_RS25270 0.34 -0.6 1,009,523 + RR42_RS25270 0.38 -0.2 1,009,524 - RR42_RS25270 0.38 -1.0 1,009,524 - RR42_RS25270 0.38 +0.2 1,009,524 - RR42_RS25270 0.38 -0.0 1,009,524 - RR42_RS25270 0.38 +0.1 1,009,524 - RR42_RS25270 0.38 +0.2 1,009,589 + RR42_RS25270 0.43 +0.3 1,009,590 - RR42_RS25270 0.43 -0.3 1,009,590 - RR42_RS25270 0.43 -2.3 1,009,793 + RR42_RS25270 0.58 -1.2 1,009,793 + RR42_RS25270 0.58 -0.4 1,009,793 + RR42_RS25270 0.58 +0.4 1,009,817 + RR42_RS25270 0.60 +2.2 1,009,862 + RR42_RS25270 0.64 +1.2 1,009,863 - RR42_RS25270 0.64 -0.6 1,010,096 + RR42_RS25270 0.81 -0.7 1,010,381 + -0.7 1,010,381 + -0.6 1,010,382 - -0.0 1,010,382 - +0.1 1,010,382 - -2.5 1,010,485 - -1.0 1,010,593 + +0.1 1,010,834 + RR42_RS25275 0.27 +0.3 1,010,835 - RR42_RS25275 0.27 +0.3 1,010,835 - RR42_RS25275 0.27 -0.2 1,010,836 + RR42_RS25275 0.27 +0.2 1,010,836 + RR42_RS25275 0.27 -0.5 1,010,875 - RR42_RS25275 0.31 -0.6 1,010,910 + RR42_RS25275 0.35 -0.3 1,010,910 + RR42_RS25275 0.35 -1.3 1,010,911 - RR42_RS25275 0.35 +1.2 1,010,911 - RR42_RS25275 0.35 -0.2 1,010,911 - RR42_RS25275 0.35 -0.2 1,010,911 - RR42_RS25275 0.35 -0.5 1,010,911 - RR42_RS25275 0.35 +0.2 1,010,911 - RR42_RS25275 0.35 -0.6 1,010,911 - RR42_RS25275 0.35 -0.4 1,010,912 + RR42_RS25275 0.35 +0.2 1,010,912 + RR42_RS25275 0.35 -0.8 1,010,913 - RR42_RS25275 0.35 -0.2 1,011,051 + RR42_RS25275 0.50 -1.2 1,011,052 - RR42_RS25275 0.51 +0.3 1,011,052 - RR42_RS25275 0.51 -0.1 1,011,052 - RR42_RS25275 0.51 +0.7 1,011,052 - RR42_RS25275 0.51 +0.1 1,011,162 + RR42_RS25275 0.63 +0.6 1,011,162 + RR42_RS25275 0.63 +0.1 1,011,163 - RR42_RS25275 0.63 +0.3 1,011,163 - RR42_RS25275 0.63 +1.2 1,011,163 - RR42_RS25275 0.63 +1.3 1,011,163 - RR42_RS25275 0.63 +0.3 1,011,163 - RR42_RS25275 0.63 -1.1 1,011,213 + RR42_RS25275 0.68 +1.2 1,011,214 - RR42_RS25275 0.68 -0.2 1,011,214 - RR42_RS25275 0.68 -0.2 1,011,225 + RR42_RS25275 0.70 -0.3 1,011,298 - RR42_RS25275 0.78 +1.0
Or see this region's nucleotide sequence