Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25250 and RR42_RS25255 are separated by 106 nucleotides RR42_RS25255 and RR42_RS25265 are separated by 965 nucleotides
RR42_RS25250: RR42_RS25250 - ABC transporter substrate-binding protein, at 1,005,237 to 1,006,223
_RS25250
RR42_RS25255: RR42_RS25255 - 1,4-butanediol diacrylate esterase, at 1,006,330 to 1,007,493
_RS25255
RR42_RS25265: RR42_RS25265 - hypothetical protein, at 1,008,459 to 1,008,830
_RS25265
Position (kb)
1006
1007
1008 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1005.407 kb on + strand, within RR42_RS25250 at 1005.408 kb on - strand, within RR42_RS25250 at 1005.408 kb on - strand, within RR42_RS25250 at 1005.668 kb on - strand, within RR42_RS25250 at 1005.892 kb on + strand, within RR42_RS25250 at 1005.892 kb on + strand, within RR42_RS25250 at 1005.893 kb on - strand, within RR42_RS25250 at 1005.927 kb on + strand, within RR42_RS25250 at 1005.959 kb on - strand, within RR42_RS25250 at 1006.141 kb on + strand at 1006.283 kb on - strand at 1006.367 kb on + strand at 1006.457 kb on + strand, within RR42_RS25255 at 1006.458 kb on - strand, within RR42_RS25255 at 1006.461 kb on + strand, within RR42_RS25255 at 1006.461 kb on + strand, within RR42_RS25255 at 1006.745 kb on + strand, within RR42_RS25255 at 1006.851 kb on + strand, within RR42_RS25255 at 1006.909 kb on - strand, within RR42_RS25255 at 1006.923 kb on - strand, within RR42_RS25255 at 1006.923 kb on - strand, within RR42_RS25255 at 1007.078 kb on + strand, within RR42_RS25255 at 1007.111 kb on + strand, within RR42_RS25255 at 1007.111 kb on + strand, within RR42_RS25255 at 1007.112 kb on - strand, within RR42_RS25255 at 1007.431 kb on + strand at 1007.645 kb on + strand at 1007.645 kb on + strand at 1007.645 kb on + strand at 1007.646 kb on - strand at 1007.726 kb on + strand at 1007.801 kb on - strand at 1007.951 kb on + strand at 1008.122 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,005,407 + RR42_RS25250 0.17 -0.6 1,005,408 - RR42_RS25250 0.17 +0.8 1,005,408 - RR42_RS25250 0.17 -0.1 1,005,668 - RR42_RS25250 0.44 +2.3 1,005,892 + RR42_RS25250 0.66 +0.6 1,005,892 + RR42_RS25250 0.66 -0.3 1,005,893 - RR42_RS25250 0.66 +0.0 1,005,927 + RR42_RS25250 0.70 +0.6 1,005,959 - RR42_RS25250 0.73 -0.2 1,006,141 + -0.5 1,006,283 - +0.3 1,006,367 + +0.6 1,006,457 + RR42_RS25255 0.11 -0.5 1,006,458 - RR42_RS25255 0.11 -0.7 1,006,461 + RR42_RS25255 0.11 -0.1 1,006,461 + RR42_RS25255 0.11 -0.3 1,006,745 + RR42_RS25255 0.36 +0.2 1,006,851 + RR42_RS25255 0.45 -0.8 1,006,909 - RR42_RS25255 0.50 -1.6 1,006,923 - RR42_RS25255 0.51 -1.7 1,006,923 - RR42_RS25255 0.51 +0.1 1,007,078 + RR42_RS25255 0.64 +1.1 1,007,111 + RR42_RS25255 0.67 +0.2 1,007,111 + RR42_RS25255 0.67 -0.2 1,007,112 - RR42_RS25255 0.67 -2.5 1,007,431 + +0.7 1,007,645 + -0.2 1,007,645 + -1.0 1,007,645 + +0.5 1,007,646 - -0.4 1,007,726 + +0.0 1,007,801 - -2.2 1,007,951 + +0.6 1,008,122 - -0.2
Or see this region's nucleotide sequence