Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25240 and RR42_RS25245 are separated by 164 nucleotides RR42_RS25245 and RR42_RS25250 are separated by 27 nucleotides RR42_RS25250 and RR42_RS25255 are separated by 106 nucleotides
RR42_RS25240: RR42_RS25240 - nitronate monooxygenase, at 1,003,381 to 1,004,388
_RS25240
RR42_RS25245: RR42_RS25245 - Asp/Glu/hydantoin racemase, at 1,004,553 to 1,005,209
_RS25245
RR42_RS25250: RR42_RS25250 - ABC transporter substrate-binding protein, at 1,005,237 to 1,006,223
_RS25250
RR42_RS25255: RR42_RS25255 - 1,4-butanediol diacrylate esterase, at 1,006,330 to 1,007,493
_RS25255
Position (kb)
1005
1006
1007 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1004.402 kb on - strand at 1004.463 kb on - strand at 1004.463 kb on - strand at 1004.496 kb on - strand at 1004.635 kb on + strand, within RR42_RS25245 at 1004.635 kb on + strand, within RR42_RS25245 at 1004.635 kb on + strand, within RR42_RS25245 at 1004.636 kb on - strand, within RR42_RS25245 at 1004.636 kb on - strand, within RR42_RS25245 at 1004.758 kb on - strand, within RR42_RS25245 at 1004.999 kb on + strand, within RR42_RS25245 at 1004.999 kb on + strand, within RR42_RS25245 at 1005.000 kb on - strand, within RR42_RS25245 at 1005.043 kb on + strand, within RR42_RS25245 at 1005.043 kb on + strand, within RR42_RS25245 at 1005.184 kb on + strand at 1005.254 kb on + strand at 1005.255 kb on - strand at 1005.255 kb on - strand at 1005.255 kb on - strand at 1005.291 kb on + strand at 1005.291 kb on + strand at 1005.407 kb on + strand, within RR42_RS25250 at 1005.408 kb on - strand, within RR42_RS25250 at 1005.408 kb on - strand, within RR42_RS25250 at 1005.668 kb on - strand, within RR42_RS25250 at 1005.892 kb on + strand, within RR42_RS25250 at 1005.892 kb on + strand, within RR42_RS25250 at 1005.893 kb on - strand, within RR42_RS25250 at 1005.927 kb on + strand, within RR42_RS25250 at 1005.959 kb on - strand, within RR42_RS25250 at 1006.141 kb on + strand at 1006.283 kb on - strand at 1006.367 kb on + strand at 1006.457 kb on + strand, within RR42_RS25255 at 1006.458 kb on - strand, within RR42_RS25255 at 1006.461 kb on + strand, within RR42_RS25255 at 1006.461 kb on + strand, within RR42_RS25255 at 1006.745 kb on + strand, within RR42_RS25255 at 1006.851 kb on + strand, within RR42_RS25255 at 1006.909 kb on - strand, within RR42_RS25255 at 1006.923 kb on - strand, within RR42_RS25255 at 1006.923 kb on - strand, within RR42_RS25255 at 1007.078 kb on + strand, within RR42_RS25255 at 1007.111 kb on + strand, within RR42_RS25255 at 1007.111 kb on + strand, within RR42_RS25255 at 1007.112 kb on - strand, within RR42_RS25255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,004,402 - -0.0 1,004,463 - +1.0 1,004,463 - +0.6 1,004,496 - -0.2 1,004,635 + RR42_RS25245 0.12 -0.8 1,004,635 + RR42_RS25245 0.12 -1.1 1,004,635 + RR42_RS25245 0.12 -0.6 1,004,636 - RR42_RS25245 0.13 -0.9 1,004,636 - RR42_RS25245 0.13 -1.2 1,004,758 - RR42_RS25245 0.31 -1.2 1,004,999 + RR42_RS25245 0.68 +0.7 1,004,999 + RR42_RS25245 0.68 +0.1 1,005,000 - RR42_RS25245 0.68 +0.2 1,005,043 + RR42_RS25245 0.75 +0.4 1,005,043 + RR42_RS25245 0.75 -0.7 1,005,184 + -1.1 1,005,254 + +1.0 1,005,255 - +0.7 1,005,255 - -0.6 1,005,255 - +1.3 1,005,291 + -0.6 1,005,291 + +1.2 1,005,407 + RR42_RS25250 0.17 -0.6 1,005,408 - RR42_RS25250 0.17 +0.8 1,005,408 - RR42_RS25250 0.17 -0.1 1,005,668 - RR42_RS25250 0.44 +2.3 1,005,892 + RR42_RS25250 0.66 +0.6 1,005,892 + RR42_RS25250 0.66 -0.3 1,005,893 - RR42_RS25250 0.66 +0.0 1,005,927 + RR42_RS25250 0.70 +0.6 1,005,959 - RR42_RS25250 0.73 -0.2 1,006,141 + -0.5 1,006,283 - +0.3 1,006,367 + +0.6 1,006,457 + RR42_RS25255 0.11 -0.5 1,006,458 - RR42_RS25255 0.11 -0.7 1,006,461 + RR42_RS25255 0.11 -0.1 1,006,461 + RR42_RS25255 0.11 -0.3 1,006,745 + RR42_RS25255 0.36 +0.2 1,006,851 + RR42_RS25255 0.45 -0.8 1,006,909 - RR42_RS25255 0.50 -1.6 1,006,923 - RR42_RS25255 0.51 -1.7 1,006,923 - RR42_RS25255 0.51 +0.1 1,007,078 + RR42_RS25255 0.64 +1.1 1,007,111 + RR42_RS25255 0.67 +0.2 1,007,111 + RR42_RS25255 0.67 -0.2 1,007,112 - RR42_RS25255 0.67 -2.5
Or see this region's nucleotide sequence