Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25195 and RR42_RS25200 are separated by 558 nucleotides RR42_RS25200 and RR42_RS25205 are separated by 375 nucleotides
RR42_RS25195: RR42_RS25195 - AMP-dependent synthetase, at 993,261 to 994,934
_RS25195
RR42_RS25200: RR42_RS25200 - TetR family transcriptional regulator, at 995,493 to 996,071
_RS25200
RR42_RS25205: RR42_RS25205 - glucose 1-dehydrogenase, at 996,447 to 997,181
_RS25205
Position (kb) 995
996
997 Strain fitness (log2 ratio) -2
-1
0
1
2 at 994.542 kb on + strand, within RR42_RS25195 at 994.542 kb on + strand, within RR42_RS25195 at 994.542 kb on + strand, within RR42_RS25195 at 994.560 kb on - strand, within RR42_RS25195 at 994.610 kb on + strand, within RR42_RS25195 at 994.611 kb on - strand, within RR42_RS25195 at 994.653 kb on + strand, within RR42_RS25195 at 994.704 kb on + strand, within RR42_RS25195 at 994.704 kb on + strand, within RR42_RS25195 at 994.705 kb on - strand, within RR42_RS25195 at 994.780 kb on + strand at 994.781 kb on - strand at 994.784 kb on + strand at 994.785 kb on - strand at 994.914 kb on - strand at 994.934 kb on + strand at 994.934 kb on + strand at 994.954 kb on + strand at 994.955 kb on - strand at 994.969 kb on - strand at 995.087 kb on - strand at 995.187 kb on + strand at 995.188 kb on - strand at 995.188 kb on - strand at 995.215 kb on - strand at 995.382 kb on - strand at 995.382 kb on - strand at 995.415 kb on + strand at 995.415 kb on + strand at 995.453 kb on + strand at 995.453 kb on + strand at 995.454 kb on - strand at 995.546 kb on + strand at 995.546 kb on + strand at 995.634 kb on + strand, within RR42_RS25200 at 995.634 kb on + strand, within RR42_RS25200 at 995.634 kb on + strand, within RR42_RS25200 at 995.635 kb on - strand, within RR42_RS25200 at 995.635 kb on - strand, within RR42_RS25200 at 995.636 kb on + strand, within RR42_RS25200 at 995.763 kb on + strand, within RR42_RS25200 at 995.764 kb on - strand, within RR42_RS25200 at 996.084 kb on + strand at 996.084 kb on + strand at 996.084 kb on + strand at 996.084 kb on + strand at 996.085 kb on - strand at 996.085 kb on - strand at 996.145 kb on + strand at 996.299 kb on + strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.355 kb on - strand at 996.618 kb on + strand, within RR42_RS25205 at 996.619 kb on - strand, within RR42_RS25205 at 996.619 kb on - strand, within RR42_RS25205 at 996.709 kb on + strand, within RR42_RS25205 at 996.872 kb on + strand, within RR42_RS25205 at 996.900 kb on + strand, within RR42_RS25205 at 996.901 kb on - strand, within RR42_RS25205 at 996.901 kb on - strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.992 kb on + strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 996.993 kb on - strand, within RR42_RS25205 at 997.026 kb on - strand, within RR42_RS25205 at 997.050 kb on + strand, within RR42_RS25205 at 997.050 kb on + strand, within RR42_RS25205
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 994,542 + RR42_RS25195 0.77 -0.5 994,542 + RR42_RS25195 0.77 +0.3 994,542 + RR42_RS25195 0.77 -0.1 994,560 - RR42_RS25195 0.78 +0.8 994,610 + RR42_RS25195 0.81 -0.0 994,611 - RR42_RS25195 0.81 +0.8 994,653 + RR42_RS25195 0.83 -0.8 994,704 + RR42_RS25195 0.86 +0.3 994,704 + RR42_RS25195 0.86 +0.1 994,705 - RR42_RS25195 0.86 -1.0 994,780 + -0.6 994,781 - +0.2 994,784 + -0.8 994,785 - -1.7 994,914 - +0.6 994,934 + -1.4 994,934 + +0.3 994,954 + -0.2 994,955 - -0.9 994,969 - -0.4 995,087 - -1.9 995,187 + -0.1 995,188 - +0.1 995,188 - -0.4 995,215 - +0.2 995,382 - -0.8 995,382 - +0.9 995,415 + +0.3 995,415 + +0.1 995,453 + +0.3 995,453 + +0.4 995,454 - -1.1 995,546 + -1.2 995,546 + -0.7 995,634 + RR42_RS25200 0.24 -0.8 995,634 + RR42_RS25200 0.24 -0.8 995,634 + RR42_RS25200 0.24 -0.7 995,635 - RR42_RS25200 0.25 +2.0 995,635 - RR42_RS25200 0.25 -0.8 995,636 + RR42_RS25200 0.25 -1.1 995,763 + RR42_RS25200 0.47 +1.0 995,764 - RR42_RS25200 0.47 -0.2 996,084 + +0.1 996,084 + -0.6 996,084 + +1.0 996,084 + +0.3 996,085 - -0.2 996,085 - +0.1 996,145 + +0.1 996,299 + -2.2 996,355 - -1.6 996,355 - -0.2 996,355 - -0.5 996,355 - -0.1 996,618 + RR42_RS25205 0.23 -0.9 996,619 - RR42_RS25205 0.23 -1.4 996,619 - RR42_RS25205 0.23 +0.4 996,709 + RR42_RS25205 0.36 -0.9 996,872 + RR42_RS25205 0.58 -1.1 996,900 + RR42_RS25205 0.62 +0.2 996,901 - RR42_RS25205 0.62 -0.0 996,901 - RR42_RS25205 0.62 -0.3 996,992 + RR42_RS25205 0.74 -0.7 996,992 + RR42_RS25205 0.74 -0.0 996,992 + RR42_RS25205 0.74 -0.6 996,992 + RR42_RS25205 0.74 -0.7 996,993 - RR42_RS25205 0.74 -0.2 996,993 - RR42_RS25205 0.74 -0.0 996,993 - RR42_RS25205 0.74 -0.7 997,026 - RR42_RS25205 0.79 -2.0 997,050 + RR42_RS25205 0.82 -0.0 997,050 + RR42_RS25205 0.82 +0.3
Or see this region's nucleotide sequence